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High catechins/low caffeine powder from green tea leaves by pressurized liquid extraction and supercritical antisolvent precipitation

Villanueva Bermejo, David; Ibanez, Elena; Reglero, Guillermo; Turner, Charlotta LU ; Fornari, Tiziana and Rodriguez Meizoso, Irene LU (2015) In Separation and Purification Technology 148. p.49-56
Abstract
This paper reports a method to produce a solvent-free extract from green tea leaves with high content of catechins and low content of caffeine, in two steps and using only "green" solvents. The method is based on the pressurized liquid extraction (PLE) of the green tea leaves using ethyl lactate as solvent, followed by a selective precipitation procedure using the supercritical carbon dioxide (SCCO2) antisolvent (SAS) technique. PLE was accomplished at 100 degrees C and 10 MPa, on the basis of a previous work. The influence of pressure (15-30 MPa) and temperature (50 degrees C and 70 degrees C) on the SAS precipitation process was experimentally studied in terms of precipitation yield, concentration of key bioactive compounds (caffeine and... (More)
This paper reports a method to produce a solvent-free extract from green tea leaves with high content of catechins and low content of caffeine, in two steps and using only "green" solvents. The method is based on the pressurized liquid extraction (PLE) of the green tea leaves using ethyl lactate as solvent, followed by a selective precipitation procedure using the supercritical carbon dioxide (SCCO2) antisolvent (SAS) technique. PLE was accomplished at 100 degrees C and 10 MPa, on the basis of a previous work. The influence of pressure (15-30 MPa) and temperature (50 degrees C and 70 degrees C) on the SAS precipitation process was experimentally studied in terms of precipitation yield, concentration of key bioactive compounds (caffeine and monomeric catechins) and total content of phenols of the precipitates. Additionally, a comparison using a different organic solvent (ethanol) for the extraction and precipitation steps is reported. The precipitates obtained from the ethyl lactate PLE extracts were decaffeinated (giving less than 1% mass caffeine in the dry matter) and the concentration of catechins was close to 23% mass. The total phenolic content of the precipitates was up to 590 mg of gallic acid equivalents per g of precipitate, which represents an increase of up to 25% with respect to the PLE extracts. (C) 2015 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Green tea, Decaffeination, Ethyl lactate, Pressurized liquid extraction, Supercritical antisolvent process
in
Separation and Purification Technology
volume
148
pages
49 - 56
publisher
Elsevier
external identifiers
  • wos:000356558400007
  • scopus:84929334542
ISSN
1873-3794
DOI
10.1016/j.seppur.2015.04.037
language
English
LU publication?
yes
id
fdbdf0e0-267c-44a0-b513-4df1d439d622 (old id 7596807)
date added to LUP
2015-07-23 11:22:01
date last changed
2017-05-21 03:15:37
@article{fdbdf0e0-267c-44a0-b513-4df1d439d622,
  abstract     = {This paper reports a method to produce a solvent-free extract from green tea leaves with high content of catechins and low content of caffeine, in two steps and using only "green" solvents. The method is based on the pressurized liquid extraction (PLE) of the green tea leaves using ethyl lactate as solvent, followed by a selective precipitation procedure using the supercritical carbon dioxide (SCCO2) antisolvent (SAS) technique. PLE was accomplished at 100 degrees C and 10 MPa, on the basis of a previous work. The influence of pressure (15-30 MPa) and temperature (50 degrees C and 70 degrees C) on the SAS precipitation process was experimentally studied in terms of precipitation yield, concentration of key bioactive compounds (caffeine and monomeric catechins) and total content of phenols of the precipitates. Additionally, a comparison using a different organic solvent (ethanol) for the extraction and precipitation steps is reported. The precipitates obtained from the ethyl lactate PLE extracts were decaffeinated (giving less than 1% mass caffeine in the dry matter) and the concentration of catechins was close to 23% mass. The total phenolic content of the precipitates was up to 590 mg of gallic acid equivalents per g of precipitate, which represents an increase of up to 25% with respect to the PLE extracts. (C) 2015 Elsevier B.V. All rights reserved.},
  author       = {Villanueva Bermejo, David and Ibanez, Elena and Reglero, Guillermo and Turner, Charlotta and Fornari, Tiziana and Rodriguez Meizoso, Irene},
  issn         = {1873-3794},
  keyword      = {Green tea,Decaffeination,Ethyl lactate,Pressurized liquid extraction,Supercritical antisolvent process},
  language     = {eng},
  pages        = {49--56},
  publisher    = {Elsevier},
  series       = {Separation and Purification Technology},
  title        = {High catechins/low caffeine powder from green tea leaves by pressurized liquid extraction and supercritical antisolvent precipitation},
  url          = {http://dx.doi.org/10.1016/j.seppur.2015.04.037},
  volume       = {148},
  year         = {2015},
}