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Identification of novel diagnostic biomarkers for deep venous thrombosis

Memon, Ashfaque A. LU ; Sundquist, Kristina LU ; PirouziFard, Mirnabi LU ; Elf, Johan L. LU ; Strandberg, Karin LU ; Svensson, Peter J. LU ; Sundquist, Jan LU and Zöller, Bengt LU (2018) In British Journal of Haematology 181(3). p.378-385
Abstract

The combination of a negative D-dimer and a Wells score can rule out, but not confirm, a diagnosis of deep venous thrombosis (DVT). We aimed to identify new diagnostic biomarkers for DVT and to investigate their relationship with hypercoagulability markers [D-dimer and activated protein C-protein C inhibitor (APC-PCI) complex]. We screened 92 cardiovascular-specific proteins in plasma samples from 45 confirmed DVT patients and 45 age- and sex-matched non-DVT patients selected from a prospective multicentre diagnostic management study (SCORE) by Proseek Multiplex CVDIII96×96. Plasma levels of 30 proteins were significantly different between DVT and non-DVT patients. After Bonferroni correction, plasma levels of seven proteins:... (More)

The combination of a negative D-dimer and a Wells score can rule out, but not confirm, a diagnosis of deep venous thrombosis (DVT). We aimed to identify new diagnostic biomarkers for DVT and to investigate their relationship with hypercoagulability markers [D-dimer and activated protein C-protein C inhibitor (APC-PCI) complex]. We screened 92 cardiovascular-specific proteins in plasma samples from 45 confirmed DVT patients and 45 age- and sex-matched non-DVT patients selected from a prospective multicentre diagnostic management study (SCORE) by Proseek Multiplex CVDIII96×96. Plasma levels of 30 proteins were significantly different between DVT and non-DVT patients. After Bonferroni correction, plasma levels of seven proteins: P-selectin, transferrin receptor protein 1, von Willebrand factor, tissue factor pathway inhibitor, osteopontin (OPN), bleomycin hydrolase and ST2 protein remained significantly different. The area under curve (AUC) for these proteins ranged from 0·70 to 0·84. Furthermore, all seven identified proteins were significantly associated with markers of hypercoagulability. A combination of OPN and APC-PCI had the best ability to discriminate DVT from non-DVT patients (AUC = 0·94; sensitivity = 89% and specificity = s84%). In conclusion, we identified multiple proteins associated with markers of hypercoagulability and with a potential to become novel diagnostic biomarkers for DVT.

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
APC-PCI, coagulation, D-dimer, deep venous thrombosis, diagnostic biomarkers
in
British Journal of Haematology
volume
181
issue
3
pages
8 pages
publisher
Federation of European Neuroscience Societies and Blackwell Publishing Ltd
external identifiers
  • scopus:85045892663
ISSN
0007-1048
DOI
10.1111/bjh.15206
language
English
LU publication?
yes
id
76cdd5a0-603e-4665-853e-1f1fb06113bd
date added to LUP
2018-05-04 07:51:50
date last changed
2019-09-11 03:56:11
@article{76cdd5a0-603e-4665-853e-1f1fb06113bd,
  abstract     = {<p>The combination of a negative D-dimer and a Wells score can rule out, but not confirm, a diagnosis of deep venous thrombosis (DVT). We aimed to identify new diagnostic biomarkers for DVT and to investigate their relationship with hypercoagulability markers [D-dimer and activated protein C-protein C inhibitor (APC-PCI) complex]. We screened 92 cardiovascular-specific proteins in plasma samples from 45 confirmed DVT patients and 45 age- and sex-matched non-DVT patients selected from a prospective multicentre diagnostic management study (SCORE) by Proseek Multiplex CVDIII<sup>96×96</sup>. Plasma levels of 30 proteins were significantly different between DVT and non-DVT patients. After Bonferroni correction, plasma levels of seven proteins: P-selectin, transferrin receptor protein 1, von Willebrand factor, tissue factor pathway inhibitor, osteopontin (OPN), bleomycin hydrolase and ST2 protein remained significantly different. The area under curve (AUC) for these proteins ranged from 0·70 to 0·84. Furthermore, all seven identified proteins were significantly associated with markers of hypercoagulability. A combination of OPN and APC-PCI had the best ability to discriminate DVT from non-DVT patients (AUC = 0·94; sensitivity = 89% and specificity = s84%). In conclusion, we identified multiple proteins associated with markers of hypercoagulability and with a potential to become novel diagnostic biomarkers for DVT.</p>},
  author       = {Memon, Ashfaque A. and Sundquist, Kristina and PirouziFard, Mirnabi and Elf, Johan L. and Strandberg, Karin and Svensson, Peter J. and Sundquist, Jan and Zöller, Bengt},
  issn         = {0007-1048},
  keyword      = {APC-PCI,coagulation,D-dimer,deep venous thrombosis,diagnostic biomarkers},
  language     = {eng},
  month        = {05},
  number       = {3},
  pages        = {378--385},
  publisher    = {Federation of European Neuroscience Societies and Blackwell Publishing Ltd},
  series       = {British Journal of Haematology},
  title        = {Identification of novel diagnostic biomarkers for deep venous thrombosis},
  url          = {http://dx.doi.org/10.1111/bjh.15206},
  volume       = {181},
  year         = {2018},
}