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Spectral Signatures in the Different Layers of the Human Eyelid by Photoacoustic Imaging

Dahlstrand, Ulf LU ; Sheikh, Rafi LU ; Berggren, Johanna LU ; Hult, Jenny LU ; Albinsson, John LU ; Cinthio, Magnus LU and Malmsjö, Malin LU (2019) In Lasers in Surgery and Medicine
Abstract

BACKGROUND AND OBJECTIVES: The eyelids are susceptible to a number of skin cancers, which are challenging to excise radically without sacrificing excessive healthy tissue. Photoacoustic (PA) imaging is an emerging non-invasive biomedical imaging modality that could potentially be used for intraoperative micrographic control of the surgical margins of eyelid tumors. In this study, non-cancerous human eyelid tissue was characterized using PA as a first step in the development of this technique.

STUDY DESIGN/MATERIALS AND METHODS: Twelve full-thickness samples from nine patients were analyzed ex vivo using PA imaging. Two-dimensional PA images were acquired using 59 wavelengths in the range of 680-970 nm to obtain the spectral... (More)

BACKGROUND AND OBJECTIVES: The eyelids are susceptible to a number of skin cancers, which are challenging to excise radically without sacrificing excessive healthy tissue. Photoacoustic (PA) imaging is an emerging non-invasive biomedical imaging modality that could potentially be used for intraoperative micrographic control of the surgical margins of eyelid tumors. In this study, non-cancerous human eyelid tissue was characterized using PA as a first step in the development of this technique.

STUDY DESIGN/MATERIALS AND METHODS: Twelve full-thickness samples from nine patients were analyzed ex vivo using PA imaging. Two-dimensional PA images were acquired using 59 wavelengths in the range of 680-970 nm to obtain the spectral signatures of the skin, orbicularis oculi muscle, and the tarsal plate. Three-dimensional images were obtained by scanning the tissues using a linear stepping motor. Spectral unmixing was performed to visualize the chromophore distribution.

RESULTS: The resulting PA spectra could be used to differentiate between the orbicularis oculi muscle and the other two structures (P < 0.05). The signals from the skin and the tarsal plate were more similar in appearance, probably due to similarities in their molecular composition. Spectral unmixing provided a clear visualization of the overall architecture of the eyelids.

CONCLUSIONS: PA imaging can be used to differentiate between the orbicularis oculi muscle and the eyelid skin and tarsal plate. The main structures of human eyelids could be visualized in three dimensions using PA imaging. This technique could potentially be used to examine eyelid tumors intraoperatively in the future. However, further studies on tumors in vivo are needed before considering such clinical use. Lasers Surg Med. © 2019 Wiley Periodicals, Inc.

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publication status
published
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in
Lasers in Surgery and Medicine
publisher
John Wiley & Sons
external identifiers
  • scopus:85070978755
  • pmid:31441078
ISSN
0196-8092
DOI
10.1002/lsm.23148
language
English
LU publication?
yes
additional info
© 2019 Wiley Periodicals, Inc.
id
76e7f8bb-e206-4efa-8c25-e4fff7991ded
date added to LUP
2019-09-04 00:05:05
date last changed
2020-01-13 02:19:47
@article{76e7f8bb-e206-4efa-8c25-e4fff7991ded,
  abstract     = {<p>BACKGROUND AND OBJECTIVES: The eyelids are susceptible to a number of skin cancers, which are challenging to excise radically without sacrificing excessive healthy tissue. Photoacoustic (PA) imaging is an emerging non-invasive biomedical imaging modality that could potentially be used for intraoperative micrographic control of the surgical margins of eyelid tumors. In this study, non-cancerous human eyelid tissue was characterized using PA as a first step in the development of this technique.</p><p>STUDY DESIGN/MATERIALS AND METHODS: Twelve full-thickness samples from nine patients were analyzed ex vivo using PA imaging. Two-dimensional PA images were acquired using 59 wavelengths in the range of 680-970 nm to obtain the spectral signatures of the skin, orbicularis oculi muscle, and the tarsal plate. Three-dimensional images were obtained by scanning the tissues using a linear stepping motor. Spectral unmixing was performed to visualize the chromophore distribution.</p><p>RESULTS: The resulting PA spectra could be used to differentiate between the orbicularis oculi muscle and the other two structures (P &lt; 0.05). The signals from the skin and the tarsal plate were more similar in appearance, probably due to similarities in their molecular composition. Spectral unmixing provided a clear visualization of the overall architecture of the eyelids.</p><p>CONCLUSIONS: PA imaging can be used to differentiate between the orbicularis oculi muscle and the eyelid skin and tarsal plate. The main structures of human eyelids could be visualized in three dimensions using PA imaging. This technique could potentially be used to examine eyelid tumors intraoperatively in the future. However, further studies on tumors in vivo are needed before considering such clinical use. Lasers Surg Med. © 2019 Wiley Periodicals, Inc.</p>},
  author       = {Dahlstrand, Ulf and Sheikh, Rafi and Berggren, Johanna and Hult, Jenny and Albinsson, John and Cinthio, Magnus and Malmsjö, Malin},
  issn         = {0196-8092},
  language     = {eng},
  month        = {08},
  publisher    = {John Wiley & Sons},
  series       = {Lasers in Surgery and Medicine},
  title        = {Spectral Signatures in the Different Layers of the Human Eyelid by Photoacoustic Imaging},
  url          = {http://dx.doi.org/10.1002/lsm.23148},
  doi          = {10.1002/lsm.23148},
  year         = {2019},
}