Driving forces for phase separation and partitioning in aqueous two-phase systems
(1998) In Journal of Chromatography. B 711(1-2). p.3-17- Abstract
- A set of simple analytical equations, derived from the Flory-Huggins theory, are used to identify the dominant driving forces for phase separation and solute (e.g., protein) partitioning, in the absence and presence of added electrolyte, in every general class of aqueous two-phase systems. The resulting model appears to capture the basic nature of two-phase systems and all trends observed experimentally. Case studies are used to identify fundamental differences in and the magnitudes of enthalpic and entropic contributions to partitioning in polymer-polymer (e.g., PEG-dextran), polymer-salt, and thermoseparating polymer-water (e.g., UCON-water) two-phase systems. The model therefore provides practitioners with a better understanding of... (More)
- A set of simple analytical equations, derived from the Flory-Huggins theory, are used to identify the dominant driving forces for phase separation and solute (e.g., protein) partitioning, in the absence and presence of added electrolyte, in every general class of aqueous two-phase systems. The resulting model appears to capture the basic nature of two-phase systems and all trends observed experimentally. Case studies are used to identify fundamental differences in and the magnitudes of enthalpic and entropic contributions to partitioning in polymer-polymer (e.g., PEG-dextran), polymer-salt, and thermoseparating polymer-water (e.g., UCON-water) two-phase systems. The model therefore provides practitioners with a better understanding of partition systems, and industry with a simple, fundamental tool for selecting an appropriate two-phase system for a particular separation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/125552
- author
- Johansson, Hans-Olof LU ; Karlström, Gunnar LU ; Tjerneld, Folke LU and Haynes, Charles A
- organization
- publishing date
- 1998
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Proteins
- in
- Journal of Chromatography. B
- volume
- 711
- issue
- 1-2
- pages
- 3 - 17
- publisher
- Elsevier
- external identifiers
-
- scopus:0032569060
- ISSN
- 1387-2273
- DOI
- 10.1016/S0378-4347(97)00585-9
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Theoretical Chemistry (S) (011001039), Biochemistry and Structural Biology (S) (000006142)
- id
- 7708fbae-0430-4464-84c0-d8a8465357d9 (old id 125552)
- date added to LUP
- 2016-04-01 12:31:42
- date last changed
- 2023-01-03 17:49:23
@article{7708fbae-0430-4464-84c0-d8a8465357d9, abstract = {{A set of simple analytical equations, derived from the Flory-Huggins theory, are used to identify the dominant driving forces for phase separation and solute (e.g., protein) partitioning, in the absence and presence of added electrolyte, in every general class of aqueous two-phase systems. The resulting model appears to capture the basic nature of two-phase systems and all trends observed experimentally. Case studies are used to identify fundamental differences in and the magnitudes of enthalpic and entropic contributions to partitioning in polymer-polymer (e.g., PEG-dextran), polymer-salt, and thermoseparating polymer-water (e.g., UCON-water) two-phase systems. The model therefore provides practitioners with a better understanding of partition systems, and industry with a simple, fundamental tool for selecting an appropriate two-phase system for a particular separation.}}, author = {{Johansson, Hans-Olof and Karlström, Gunnar and Tjerneld, Folke and Haynes, Charles A}}, issn = {{1387-2273}}, keywords = {{Proteins}}, language = {{eng}}, number = {{1-2}}, pages = {{3--17}}, publisher = {{Elsevier}}, series = {{Journal of Chromatography. B}}, title = {{Driving forces for phase separation and partitioning in aqueous two-phase systems}}, url = {{http://dx.doi.org/10.1016/S0378-4347(97)00585-9}}, doi = {{10.1016/S0378-4347(97)00585-9}}, volume = {{711}}, year = {{1998}}, }