Isolation of high yield and quality RNA from human precision-cut lung slices for RNA-sequencing and computational integration with larger patient cohorts
(2021) In American Journal of Physiology: Lung Cellular and Molecular Physiology 320(2). p.232-240- Abstract
Precision-cut lung slices (PCLS) have gained increasing interest as a model to study lung biology/disease and screening novel therapeutics. In particular, PCLS derived from human tissue can better recapitulate some aspects of lung biology/disease as compared to animal models. Several experimental readouts have been established for use with PCLS, but obtaining high yield and quality RNA for downstream analysis has remained challenging. This is particularly problematic for utilizing the power of next-generation sequencing techniques, such as RNA-sequencing (RNA-seq), for non-biased and high through-put analysis of PCLS human cohorts. In the current study, we present a novel approach for isolating high quality RNA from a small amount of... (More)
Precision-cut lung slices (PCLS) have gained increasing interest as a model to study lung biology/disease and screening novel therapeutics. In particular, PCLS derived from human tissue can better recapitulate some aspects of lung biology/disease as compared to animal models. Several experimental readouts have been established for use with PCLS, but obtaining high yield and quality RNA for downstream analysis has remained challenging. This is particularly problematic for utilizing the power of next-generation sequencing techniques, such as RNA-sequencing (RNA-seq), for non-biased and high through-put analysis of PCLS human cohorts. In the current study, we present a novel approach for isolating high quality RNA from a small amount of tissue, including diseased human tissue, such as idiopathic pulmonary fibrosis (IPF). We show that the RNA isolated using this method has sufficient quality for RT-qPCR and RNA-seq analysis. Furthermore, the RNA-seq data from human PCLS could be used in several established computational pipelines, including deconvolution of bulk RNA-seq data using publicly available single-cell RNA-seq data. Deconvolution using Bisque revealed a diversity of cell populations in human PCLS, including several immune cell populations, which correlated with cell populations known to be present and aberrant in human disease.
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- author
- Stegmayr, John LU ; Alsafadi, Hani N LU ; Langwinski, Wojciech LU ; Niroomand, Anna LU ; Lindstedt, Sandra LU ; Leigh, Nicholas D LU and Wagner, Darcy E LU
- organization
-
- Lung Bioengineering and Regeneration (research group)
- Lund University Bioimaging Center
- StemTherapy: National Initiative on Stem Cells for Regenerative Therapy
- WCMM-Wallenberg Centre for Molecular Medicine
- Thoracic Surgery
- Clinical and experimental lung transplantation (research group)
- Regenerative Immunology (research group)
- Division of Molecular Medicine and Gene Therapy
- publishing date
- 2021
- type
- Contribution to journal
- publication status
- published
- subject
- in
- American Journal of Physiology: Lung Cellular and Molecular Physiology
- volume
- 320
- issue
- 2
- pages
- 232 - 240
- publisher
- American Physiological Society
- external identifiers
-
- pmid:33112185
- scopus:85102213115
- ISSN
- 1522-1504
- DOI
- 10.1152/ajplung.00401.2020
- language
- English
- LU publication?
- yes
- id
- 7722000c-549f-4a1d-8a0c-b2eaae469303
- date added to LUP
- 2020-11-05 10:12:20
- date last changed
- 2024-09-19 08:12:50
@article{7722000c-549f-4a1d-8a0c-b2eaae469303, abstract = {{<p>Precision-cut lung slices (PCLS) have gained increasing interest as a model to study lung biology/disease and screening novel therapeutics. In particular, PCLS derived from human tissue can better recapitulate some aspects of lung biology/disease as compared to animal models. Several experimental readouts have been established for use with PCLS, but obtaining high yield and quality RNA for downstream analysis has remained challenging. This is particularly problematic for utilizing the power of next-generation sequencing techniques, such as RNA-sequencing (RNA-seq), for non-biased and high through-put analysis of PCLS human cohorts. In the current study, we present a novel approach for isolating high quality RNA from a small amount of tissue, including diseased human tissue, such as idiopathic pulmonary fibrosis (IPF). We show that the RNA isolated using this method has sufficient quality for RT-qPCR and RNA-seq analysis. Furthermore, the RNA-seq data from human PCLS could be used in several established computational pipelines, including deconvolution of bulk RNA-seq data using publicly available single-cell RNA-seq data. Deconvolution using Bisque revealed a diversity of cell populations in human PCLS, including several immune cell populations, which correlated with cell populations known to be present and aberrant in human disease.</p>}}, author = {{Stegmayr, John and Alsafadi, Hani N and Langwinski, Wojciech and Niroomand, Anna and Lindstedt, Sandra and Leigh, Nicholas D and Wagner, Darcy E}}, issn = {{1522-1504}}, language = {{eng}}, number = {{2}}, pages = {{232--240}}, publisher = {{American Physiological Society}}, series = {{American Journal of Physiology: Lung Cellular and Molecular Physiology}}, title = {{Isolation of high yield and quality RNA from human precision-cut lung slices for RNA-sequencing and computational integration with larger patient cohorts}}, url = {{http://dx.doi.org/10.1152/ajplung.00401.2020}}, doi = {{10.1152/ajplung.00401.2020}}, volume = {{320}}, year = {{2021}}, }