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From 1D Rods to 3D Networks: A Biohybrid Topological Diversity Investigated by Asymmetrical Flow Field-Flow Fractionation

Boye, Susanne; Ennen, Franka; Scharfenberg, Linda; Appelhans, Dietmar; Nilsson, Lars LU and Lederer, Albena (2015) In Macromolecules 48(13). p.4607-4619
Abstract
Biohybrid structures formed by noncovalent interaction between avidin as a bridging unit and biotinylated glycodendrimers based on poly(propyleneimine) (GD-B) have potential for biomedical application. Therefore, an exact knowledge about molar mass, dispersity, size, shape, and molecular structure is required. Asymmetrical flow field-flow fractionation (AF4) was applied to separate pure and assembled macromolecules according to their diffusion coefficients. The complex biohybrid structures consist of single components (avidin, differently valent GD-B) and nanostructures. These nanostructures were systematically studied depending on the degree of biotinylation and ligand-receptor stoichiometry by AF4 in combination with dynamic and static... (More)
Biohybrid structures formed by noncovalent interaction between avidin as a bridging unit and biotinylated glycodendrimers based on poly(propyleneimine) (GD-B) have potential for biomedical application. Therefore, an exact knowledge about molar mass, dispersity, size, shape, and molecular structure is required. Asymmetrical flow field-flow fractionation (AF4) was applied to separate pure and assembled macromolecules according to their diffusion coefficients. The complex biohybrid structures consist of single components (avidin, differently valent GD-B) and nanostructures. These nanostructures were systematically studied depending on the degree of biotinylation and ligand-receptor stoichiometry by AF4 in combination with dynamic and static light scattering detection. This enables the quantification of composition and calculation of molar masses and radii, which were used to analyze scaling properties and apparent density of the formed structures. These data are compared to hydrodynamic radii obtained by applying the retention theory to the AF4 data. It is shown that depending on their architecture the molecular shape of biohybrid structures is changed from rod-like to spherical toward network-like behavior. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Macromolecules
volume
48
issue
13
pages
4607 - 4619
publisher
The American Chemical Society
external identifiers
  • wos:000358104900040
  • scopus:84937046373
ISSN
0024-9297
DOI
10.1021/acs.macromol.5b00824
language
English
LU publication?
yes
id
a17a0742-3c5f-4819-acee-7e9cbf1298df (old id 7779828)
date added to LUP
2015-09-18 16:05:23
date last changed
2017-01-01 04:05:12
@article{a17a0742-3c5f-4819-acee-7e9cbf1298df,
  abstract     = {Biohybrid structures formed by noncovalent interaction between avidin as a bridging unit and biotinylated glycodendrimers based on poly(propyleneimine) (GD-B) have potential for biomedical application. Therefore, an exact knowledge about molar mass, dispersity, size, shape, and molecular structure is required. Asymmetrical flow field-flow fractionation (AF4) was applied to separate pure and assembled macromolecules according to their diffusion coefficients. The complex biohybrid structures consist of single components (avidin, differently valent GD-B) and nanostructures. These nanostructures were systematically studied depending on the degree of biotinylation and ligand-receptor stoichiometry by AF4 in combination with dynamic and static light scattering detection. This enables the quantification of composition and calculation of molar masses and radii, which were used to analyze scaling properties and apparent density of the formed structures. These data are compared to hydrodynamic radii obtained by applying the retention theory to the AF4 data. It is shown that depending on their architecture the molecular shape of biohybrid structures is changed from rod-like to spherical toward network-like behavior.},
  author       = {Boye, Susanne and Ennen, Franka and Scharfenberg, Linda and Appelhans, Dietmar and Nilsson, Lars and Lederer, Albena},
  issn         = {0024-9297},
  language     = {eng},
  number       = {13},
  pages        = {4607--4619},
  publisher    = {The American Chemical Society},
  series       = {Macromolecules},
  title        = {From 1D Rods to 3D Networks: A Biohybrid Topological Diversity Investigated by Asymmetrical Flow Field-Flow Fractionation},
  url          = {http://dx.doi.org/10.1021/acs.macromol.5b00824},
  volume       = {48},
  year         = {2015},
}