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Alpha-Synuclein Expression in the Oligodendrocyte Lineage: an In Vitro and In Vivo Study Using Rodent and Human Models.

Djelloul, Mehdi LU ; Holmqvist, Staffan LU ; Boza Serrano, Antonio LU ; Azevedo, Carla LU ; Yeung, Maggie S; Goldwurm, Stefano; Frisén, Jonas; Deierborg, Tomas LU and Roybon, Laurent LU (2015) In Stem Cell Reports 5(2). p.174-184
Abstract
In this study, we sought evidence for alpha-synuclein (ASYN) expression in oligodendrocytes, as a possible endogenous source of ASYN to explain its presence in glial inclusions found in multiple system atrophy (MSA) and Parkinson's disease (PD). We identified ASYN in oligodendrocyte lineage progenitors isolated from the rodent brain, in oligodendrocytes generated from embryonic stem cells, and in induced pluripotent stem cells produced from fibroblasts of a healthy individual and patients diagnosed with MSA or PD, in cultures in vitro. Notably, we observed a significant decrease in ΑSYN during oligodendrocyte maturation. Additionally, we show the presence of transcripts in PDGFRΑ/CD140a(+) cells and SOX10(+) oligodendrocyte lineage nuclei... (More)
In this study, we sought evidence for alpha-synuclein (ASYN) expression in oligodendrocytes, as a possible endogenous source of ASYN to explain its presence in glial inclusions found in multiple system atrophy (MSA) and Parkinson's disease (PD). We identified ASYN in oligodendrocyte lineage progenitors isolated from the rodent brain, in oligodendrocytes generated from embryonic stem cells, and in induced pluripotent stem cells produced from fibroblasts of a healthy individual and patients diagnosed with MSA or PD, in cultures in vitro. Notably, we observed a significant decrease in ΑSYN during oligodendrocyte maturation. Additionally, we show the presence of transcripts in PDGFRΑ/CD140a(+) cells and SOX10(+) oligodendrocyte lineage nuclei isolated by FACS from rodent and human healthy and diseased brains, respectively. Our work identifies ASYN in oligodendrocyte lineage cells, and it offers additional in vitro cellular models that should provide significant insights of the functional implication of ASYN during oligodendrocyte development and disease. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Stem Cell Reports
volume
5
issue
2
pages
174 - 184
publisher
Cell Press
external identifiers
  • pmid:26235891
  • wos:000359428800003
  • scopus:84954168300
ISSN
2213-6711
DOI
10.1016/j.stemcr.2015.07.002
language
English
LU publication?
yes
id
f84974af-ac7e-42e3-b6ba-751c123afe19 (old id 7844983)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26235891?dopt=Abstract
date added to LUP
2015-09-05 12:04:29
date last changed
2017-09-10 04:16:24
@article{f84974af-ac7e-42e3-b6ba-751c123afe19,
  abstract     = {In this study, we sought evidence for alpha-synuclein (ASYN) expression in oligodendrocytes, as a possible endogenous source of ASYN to explain its presence in glial inclusions found in multiple system atrophy (MSA) and Parkinson's disease (PD). We identified ASYN in oligodendrocyte lineage progenitors isolated from the rodent brain, in oligodendrocytes generated from embryonic stem cells, and in induced pluripotent stem cells produced from fibroblasts of a healthy individual and patients diagnosed with MSA or PD, in cultures in vitro. Notably, we observed a significant decrease in ΑSYN during oligodendrocyte maturation. Additionally, we show the presence of transcripts in PDGFRΑ/CD140a(+) cells and SOX10(+) oligodendrocyte lineage nuclei isolated by FACS from rodent and human healthy and diseased brains, respectively. Our work identifies ASYN in oligodendrocyte lineage cells, and it offers additional in vitro cellular models that should provide significant insights of the functional implication of ASYN during oligodendrocyte development and disease.},
  author       = {Djelloul, Mehdi and Holmqvist, Staffan and Boza Serrano, Antonio and Azevedo, Carla and Yeung, Maggie S and Goldwurm, Stefano and Frisén, Jonas and Deierborg, Tomas and Roybon, Laurent},
  issn         = {2213-6711},
  language     = {eng},
  number       = {2},
  pages        = {174--184},
  publisher    = {Cell Press},
  series       = {Stem Cell Reports},
  title        = {Alpha-Synuclein Expression in the Oligodendrocyte Lineage: an In Vitro and In Vivo Study Using Rodent and Human Models.},
  url          = {http://dx.doi.org/10.1016/j.stemcr.2015.07.002},
  volume       = {5},
  year         = {2015},
}