Alpha-Synuclein Expression in the Oligodendrocyte Lineage: an In Vitro and In Vivo Study Using Rodent and Human Models.
(2015) In Stem Cell Reports 5(2). p.174-184- Abstract
- In this study, we sought evidence for alpha-synuclein (ASYN) expression in oligodendrocytes, as a possible endogenous source of ASYN to explain its presence in glial inclusions found in multiple system atrophy (MSA) and Parkinson's disease (PD). We identified ASYN in oligodendrocyte lineage progenitors isolated from the rodent brain, in oligodendrocytes generated from embryonic stem cells, and in induced pluripotent stem cells produced from fibroblasts of a healthy individual and patients diagnosed with MSA or PD, in cultures in vitro. Notably, we observed a significant decrease in ΑSYN during oligodendrocyte maturation. Additionally, we show the presence of transcripts in PDGFRΑ/CD140a(+) cells and SOX10(+) oligodendrocyte lineage nuclei... (More)
- In this study, we sought evidence for alpha-synuclein (ASYN) expression in oligodendrocytes, as a possible endogenous source of ASYN to explain its presence in glial inclusions found in multiple system atrophy (MSA) and Parkinson's disease (PD). We identified ASYN in oligodendrocyte lineage progenitors isolated from the rodent brain, in oligodendrocytes generated from embryonic stem cells, and in induced pluripotent stem cells produced from fibroblasts of a healthy individual and patients diagnosed with MSA or PD, in cultures in vitro. Notably, we observed a significant decrease in ΑSYN during oligodendrocyte maturation. Additionally, we show the presence of transcripts in PDGFRΑ/CD140a(+) cells and SOX10(+) oligodendrocyte lineage nuclei isolated by FACS from rodent and human healthy and diseased brains, respectively. Our work identifies ASYN in oligodendrocyte lineage cells, and it offers additional in vitro cellular models that should provide significant insights of the functional implication of ASYN during oligodendrocyte development and disease. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/7844983
- author
- Djelloul, Mehdi LU ; Holmqvist, Staffan LU ; Boza Serrano, Antonio LU ; Azevedo, Carla LU ; Yeung, Maggie S ; Goldwurm, Stefano ; Frisén, Jonas ; Deierborg, Tomas LU and Roybon, Laurent LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Stem Cell Reports
- volume
- 5
- issue
- 2
- pages
- 174 - 184
- publisher
- Cell Press
- external identifiers
-
- pmid:26235891
- wos:000359428800003
- scopus:84954168300
- pmid:26235891
- ISSN
- 2213-6711
- DOI
- 10.1016/j.stemcr.2015.07.002
- language
- English
- LU publication?
- yes
- id
- f84974af-ac7e-42e3-b6ba-751c123afe19 (old id 7844983)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/26235891?dopt=Abstract
- date added to LUP
- 2016-04-01 14:34:22
- date last changed
- 2024-02-25 16:05:09
@article{f84974af-ac7e-42e3-b6ba-751c123afe19, abstract = {{In this study, we sought evidence for alpha-synuclein (ASYN) expression in oligodendrocytes, as a possible endogenous source of ASYN to explain its presence in glial inclusions found in multiple system atrophy (MSA) and Parkinson's disease (PD). We identified ASYN in oligodendrocyte lineage progenitors isolated from the rodent brain, in oligodendrocytes generated from embryonic stem cells, and in induced pluripotent stem cells produced from fibroblasts of a healthy individual and patients diagnosed with MSA or PD, in cultures in vitro. Notably, we observed a significant decrease in ΑSYN during oligodendrocyte maturation. Additionally, we show the presence of transcripts in PDGFRΑ/CD140a(+) cells and SOX10(+) oligodendrocyte lineage nuclei isolated by FACS from rodent and human healthy and diseased brains, respectively. Our work identifies ASYN in oligodendrocyte lineage cells, and it offers additional in vitro cellular models that should provide significant insights of the functional implication of ASYN during oligodendrocyte development and disease.}}, author = {{Djelloul, Mehdi and Holmqvist, Staffan and Boza Serrano, Antonio and Azevedo, Carla and Yeung, Maggie S and Goldwurm, Stefano and Frisén, Jonas and Deierborg, Tomas and Roybon, Laurent}}, issn = {{2213-6711}}, language = {{eng}}, number = {{2}}, pages = {{174--184}}, publisher = {{Cell Press}}, series = {{Stem Cell Reports}}, title = {{Alpha-Synuclein Expression in the Oligodendrocyte Lineage: an In Vitro and In Vivo Study Using Rodent and Human Models.}}, url = {{https://lup.lub.lu.se/search/files/4042886/8619022.pdf}}, doi = {{10.1016/j.stemcr.2015.07.002}}, volume = {{5}}, year = {{2015}}, }