Advanced

Reduced diversity in the early fecal microbiota of infants developing atopic eczema

Wang, Mei LU ; Linninge, Caroline LU ; Olsson, Crister LU ; Adlerberth, Ingegerd; Wold, Agnes; Strachan, David P; Martricardi, Paolo M; Åberg, Nils; Perkin, Michael R and Tripodi, Salvatore, et al. (2008) In Journal of Allergy and Clinical Immunology 121(1). p.129-134
Abstract
Background



It might be that early intestinal colonization by bacteria in westernized infants fails to give rise to sufficient immune stimulation to support maturation of regulatory immune mechanisms.

Objective



The purpose of the present study was to characterize the very early infantile microbiota by using a culture-independent approach and to relate the colonization pattern to development of atopic eczema in the first 18 months of life.

Methods



Fecal samples were collected from 35 infants at 1 week of age. Twenty infants were healthy, and 15 infants were given diagnoses of atopic eczema at the age of 18 months. The fecal microbiota of the infants was compared by... (More)
Background



It might be that early intestinal colonization by bacteria in westernized infants fails to give rise to sufficient immune stimulation to support maturation of regulatory immune mechanisms.

Objective



The purpose of the present study was to characterize the very early infantile microbiota by using a culture-independent approach and to relate the colonization pattern to development of atopic eczema in the first 18 months of life.

Methods



Fecal samples were collected from 35 infants at 1 week of age. Twenty infants were healthy, and 15 infants were given diagnoses of atopic eczema at the age of 18 months. The fecal microbiota of the infants was compared by means of terminal restriction fragment length polymorphism (T-RFLP) and temporal temperature gradient gel electrophoresis (TTGE) analysis of amplified 16S rRNA genes.

Results



By means of T-RFLP analysis, the median number of peaks, Shannon-Wiener index, and Simpson index of diversity were significantly less for infants with atopic eczema than for infants remaining healthy in the whole group and for the Swedish infants when AluI was used for digestion. The same was found when TTGE patterns were compared. In addition, TTGE analysis showed significantly less bands and lower diversity indices for the British atopic infants compared with those of the control subjects.

Conclusion



There is a reduced diversity in the early fecal microbiota of infants with atopic eczema during the first 18 months of life. (Less)
Please use this url to cite or link to this publication:
author
, et al. (More)
(Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Atopic eczema, intestinal microbiota, diversity, terminal restriction fragment length polymorphism, temporal temperature gradient gel electrophoresis
in
Journal of Allergy and Clinical Immunology
volume
121
issue
1
pages
129 - 134
publisher
Elsevier
external identifiers
  • wos:000252372000019
  • scopus:38149069478
ISSN
1097-6825
DOI
10.1016/j.jaci.2007.09.011
language
English
LU publication?
yes
id
ea65bf7f-344d-4f45-b16f-df28d6b8cf7b (old id 790866)
date added to LUP
2008-01-09 11:50:11
date last changed
2017-09-10 04:14:08
@article{ea65bf7f-344d-4f45-b16f-df28d6b8cf7b,
  abstract     = {Background<br/><br>
<br/><br>
It might be that early intestinal colonization by bacteria in westernized infants fails to give rise to sufficient immune stimulation to support maturation of regulatory immune mechanisms.<br/><br>
Objective<br/><br>
<br/><br>
The purpose of the present study was to characterize the very early infantile microbiota by using a culture-independent approach and to relate the colonization pattern to development of atopic eczema in the first 18 months of life.<br/><br>
Methods<br/><br>
<br/><br>
Fecal samples were collected from 35 infants at 1 week of age. Twenty infants were healthy, and 15 infants were given diagnoses of atopic eczema at the age of 18 months. The fecal microbiota of the infants was compared by means of terminal restriction fragment length polymorphism (T-RFLP) and temporal temperature gradient gel electrophoresis (TTGE) analysis of amplified 16S rRNA genes.<br/><br>
Results<br/><br>
<br/><br>
By means of T-RFLP analysis, the median number of peaks, Shannon-Wiener index, and Simpson index of diversity were significantly less for infants with atopic eczema than for infants remaining healthy in the whole group and for the Swedish infants when AluI was used for digestion. The same was found when TTGE patterns were compared. In addition, TTGE analysis showed significantly less bands and lower diversity indices for the British atopic infants compared with those of the control subjects.<br/><br>
Conclusion<br/><br>
<br/><br>
There is a reduced diversity in the early fecal microbiota of infants with atopic eczema during the first 18 months of life.},
  author       = {Wang, Mei and Linninge, Caroline and Olsson, Crister and Adlerberth, Ingegerd and Wold, Agnes and Strachan, David P and Martricardi, Paolo M and Åberg, Nils and Perkin, Michael R and Tripodi, Salvatore and Coates, Anthony R and Hesselmar, Bill and Saalman, Robert and Molin, Göran and Ahrné, Siv},
  issn         = {1097-6825},
  keyword      = {Atopic eczema,intestinal microbiota,diversity,terminal restriction fragment length polymorphism,temporal temperature gradient gel electrophoresis},
  language     = {eng},
  number       = {1},
  pages        = {129--134},
  publisher    = {Elsevier},
  series       = {Journal of Allergy and Clinical Immunology},
  title        = {Reduced diversity in the early fecal microbiota of infants developing atopic eczema},
  url          = {http://dx.doi.org/10.1016/j.jaci.2007.09.011},
  volume       = {121},
  year         = {2008},
}