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Thylakoids reduce body fat and fat cell size by binding to dietary fat making it less available for absorption in high-fat fed mice

Stenkula, Karin G. LU ; Stenblom, Eva Lena LU ; Montelius, Caroline LU ; Egecioglu, Emil LU and Erlanson-Albertsson, Charlotte LU (2017) In Nutrition and Metabolism 14(1).
Abstract

Background: Dietary thylakoids derived from spinach have beneficial effects on body fat accumulation and blood lipids as demonstrated in humans and rodents. Important mechanisms established include delayed fat digestion in the intestine, without causing steatorrhea, and increased fatty acid oxidation in intestinal cells. The objective of our study was to elucidate if increased fecal fat excretion is an important mechanism to normalize adipose tissue metabolism during high-fat feeding in mice supplemented with thylakoids. Methods: Mice were randomized to receive HFD or thylHFD for 14 days (n = 14 for the control group and 16 for the thylakoid group). The effect of thylakoids on body fat distribution, faecal and liver fat content, and... (More)

Background: Dietary thylakoids derived from spinach have beneficial effects on body fat accumulation and blood lipids as demonstrated in humans and rodents. Important mechanisms established include delayed fat digestion in the intestine, without causing steatorrhea, and increased fatty acid oxidation in intestinal cells. The objective of our study was to elucidate if increased fecal fat excretion is an important mechanism to normalize adipose tissue metabolism during high-fat feeding in mice supplemented with thylakoids. Methods: Mice were randomized to receive HFD or thylHFD for 14 days (n = 14 for the control group and 16 for the thylakoid group). The effect of thylakoids on body fat distribution, faecal and liver fat content, and adipose tissue metabolism was investigated following high-fat feeding. Results: Thylakoid supplementation for 14 days caused an increased faecal fat content without compensatory eating compared to control. As a result, thylakoid treated animals had reduced fat mass depots and reduced liver fat accumulation compared to control. The size distribution of adipocytes isolated from visceral adipose tissue was narrowed and the cell size decreased. Adipocytes isolated from thylakoid-treated mice displayed a significantly increased lipogenesis, and protein expression of peroxisome proliferator-activated receptor gamma (PPARγ), down-stream target FAS, as well as transcription factor coactivators PGC1-α and LPIN-1 were upregulated in adipose tissue from thylakoid-fed mice. Conclusions: Together, these data suggest that thylakoid supplementation reduces body fat and fat cell size by binding to dietary fat and increasing its fecal excretion, thus reducing dietary fat available for absorption.

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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
Adipose cell size, FAS, Fatty liver disease, PGC-1α, PPARγ
in
Nutrition and Metabolism
volume
14
issue
1
publisher
Karger
external identifiers
  • scopus:85010002182
  • wos:000392252500001
ISSN
1743-7075
DOI
10.1186/s12986-016-0160-4
language
English
LU publication?
yes
id
79395cb5-47b2-4793-9862-aa67e85f5672
date added to LUP
2017-02-10 14:14:30
date last changed
2018-01-07 11:49:11
@article{79395cb5-47b2-4793-9862-aa67e85f5672,
  abstract     = {<p>Background: Dietary thylakoids derived from spinach have beneficial effects on body fat accumulation and blood lipids as demonstrated in humans and rodents. Important mechanisms established include delayed fat digestion in the intestine, without causing steatorrhea, and increased fatty acid oxidation in intestinal cells. The objective of our study was to elucidate if increased fecal fat excretion is an important mechanism to normalize adipose tissue metabolism during high-fat feeding in mice supplemented with thylakoids. Methods: Mice were randomized to receive HFD or thylHFD for 14 days (n = 14 for the control group and 16 for the thylakoid group). The effect of thylakoids on body fat distribution, faecal and liver fat content, and adipose tissue metabolism was investigated following high-fat feeding. Results: Thylakoid supplementation for 14 days caused an increased faecal fat content without compensatory eating compared to control. As a result, thylakoid treated animals had reduced fat mass depots and reduced liver fat accumulation compared to control. The size distribution of adipocytes isolated from visceral adipose tissue was narrowed and the cell size decreased. Adipocytes isolated from thylakoid-treated mice displayed a significantly increased lipogenesis, and protein expression of peroxisome proliferator-activated receptor gamma (PPARγ), down-stream target FAS, as well as transcription factor coactivators PGC1-α and LPIN-1 were upregulated in adipose tissue from thylakoid-fed mice. Conclusions: Together, these data suggest that thylakoid supplementation reduces body fat and fat cell size by binding to dietary fat and increasing its fecal excretion, thus reducing dietary fat available for absorption.</p>},
  articleno    = {4},
  author       = {Stenkula, Karin G. and Stenblom, Eva Lena and Montelius, Caroline and Egecioglu, Emil and Erlanson-Albertsson, Charlotte},
  issn         = {1743-7075},
  keyword      = {Adipose cell size,FAS,Fatty liver disease,PGC-1α,PPARγ},
  language     = {eng},
  month        = {01},
  number       = {1},
  publisher    = {Karger},
  series       = {Nutrition and Metabolism},
  title        = {Thylakoids reduce body fat and fat cell size by binding to dietary fat making it less available for absorption in high-fat fed mice},
  url          = {http://dx.doi.org/10.1186/s12986-016-0160-4},
  volume       = {14},
  year         = {2017},
}