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ENSAM: Europium Nanoparticles for Signal Enhancement of Antibody Microarrays on Nanoporous Silicon.

Järås, Kerstin LU ; Tajudin, Asilah ; Ressine, Anton LU ; Soukka, Tero ; Marko-Varga, György LU ; Bjartell, Anders LU ; Malm, Johan LU ; Laurell, Thomas LU and Lilja, Hans LU orcid (2008) In Journal of Proteome Research 7. p.1308-1314
Abstract
To improve the sensitivity of antibody microarray assays, we developed ENSAM ( Europium Nanoparticles for Signal enhancement of Antibody Microarrays). ENSAM is based on two nanomaterials. The first is polystyrene nanoparticles incorporated with europium chelate (beta-diketone) and coated with streptavidin. The multiple fluorophores incorporated into each nanoparticle should increase signal obtained from a single binding event. The second nanomaterial is array surfaces of nanoporous silicon, which creates high capacity for antibody adsorption. Two antibody microarray assays were compared: ENSAM and use of streptavidin labeled with a nine-dentate europium chelate. Analyzing biotinylated prostate-specific antigen (PSA) spiked into human... (More)
To improve the sensitivity of antibody microarray assays, we developed ENSAM ( Europium Nanoparticles for Signal enhancement of Antibody Microarrays). ENSAM is based on two nanomaterials. The first is polystyrene nanoparticles incorporated with europium chelate (beta-diketone) and coated with streptavidin. The multiple fluorophores incorporated into each nanoparticle should increase signal obtained from a single binding event. The second nanomaterial is array surfaces of nanoporous silicon, which creates high capacity for antibody adsorption. Two antibody microarray assays were compared: ENSAM and use of streptavidin labeled with a nine-dentate europium chelate. Analyzing biotinylated prostate-specific antigen (PSA) spiked into human female serum, ENSAM yielded a 10-fold signal enhancement compared to the streptavidin-europium chelate. Similarly, we observed around 1 order of magnitude greater sensitivity for the ENSAM assay (limit of detection </= 0.14 ng/mL, dynamic range > 10 (5)) compared to the streptavidin-europium chelate assay (limit of detection </= 0.7 ng/mL, dynamic range > 10 (4)). Analysis of a titration series showed strong linearity of ENSAM ( R (2) = 0.99 by linear regression). This work demonstrates the novel utility of nanoparticles with time-resolved fluorescence for signal enhancement of antibody microarrays, requiring as low as 100-200 zmol biotinylated PSA per microarray spot. In addition, proof of principle was shown for analyzing PSA in plasma obtained from patients undergoing clinical PSA-testing. (Less)
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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Proteome Research
volume
7
pages
1308 - 1314
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:18257515
  • wos:000253825100044
  • scopus:44449165077
  • pmid:18257515
ISSN
1535-3893
DOI
10.1021/pr700591j
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004), Clinical Chemistry, Malmö (013016000), Biomedical Engineering (011200011), Division of Urological Cancers (013243420)
id
7aa9dfcc-424a-43a7-af66-fde689fb1786 (old id 1042152)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18257515?dopt=Abstract
date added to LUP
2016-04-04 08:36:57
date last changed
2022-03-23 02:42:23
@article{7aa9dfcc-424a-43a7-af66-fde689fb1786,
  abstract     = {{To improve the sensitivity of antibody microarray assays, we developed ENSAM ( Europium Nanoparticles for Signal enhancement of Antibody Microarrays). ENSAM is based on two nanomaterials. The first is polystyrene nanoparticles incorporated with europium chelate (beta-diketone) and coated with streptavidin. The multiple fluorophores incorporated into each nanoparticle should increase signal obtained from a single binding event. The second nanomaterial is array surfaces of nanoporous silicon, which creates high capacity for antibody adsorption. Two antibody microarray assays were compared: ENSAM and use of streptavidin labeled with a nine-dentate europium chelate. Analyzing biotinylated prostate-specific antigen (PSA) spiked into human female serum, ENSAM yielded a 10-fold signal enhancement compared to the streptavidin-europium chelate. Similarly, we observed around 1 order of magnitude greater sensitivity for the ENSAM assay (limit of detection &lt;/= 0.14 ng/mL, dynamic range &gt; 10 (5)) compared to the streptavidin-europium chelate assay (limit of detection &lt;/= 0.7 ng/mL, dynamic range &gt; 10 (4)). Analysis of a titration series showed strong linearity of ENSAM ( R (2) = 0.99 by linear regression). This work demonstrates the novel utility of nanoparticles with time-resolved fluorescence for signal enhancement of antibody microarrays, requiring as low as 100-200 zmol biotinylated PSA per microarray spot. In addition, proof of principle was shown for analyzing PSA in plasma obtained from patients undergoing clinical PSA-testing.}},
  author       = {{Järås, Kerstin and Tajudin, Asilah and Ressine, Anton and Soukka, Tero and Marko-Varga, György and Bjartell, Anders and Malm, Johan and Laurell, Thomas and Lilja, Hans}},
  issn         = {{1535-3893}},
  language     = {{eng}},
  pages        = {{1308--1314}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Journal of Proteome Research}},
  title        = {{ENSAM: Europium Nanoparticles for Signal Enhancement of Antibody Microarrays on Nanoporous Silicon.}},
  url          = {{http://dx.doi.org/10.1021/pr700591j}},
  doi          = {{10.1021/pr700591j}},
  volume       = {{7}},
  year         = {{2008}},
}