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Morphology of living cells cultured on nanowire arrays with varying nanowire densities and diameters

Li, Zhen LU ; Persson, Henrik LU ; Adolfsson, Karl LU ; Oredsson, Stina LU and Prinz, Christelle N. LU (2018) In Science China Life Sciences p.1-9
Abstract

Vertical nanowire arrays are increasingly investigated for their applications in steering cell behavior. The geometry of the array is an important parameter, which influences the morphology and adhesion of cells. Here, we investigate the effects of array geometry on the morphology of MCF7 cancer cells and MCF10A normal-like epithelial cells. Different gallium phosphide nanowire array-geometries were produced by varying the nanowire density and diameter. Our results show that the cell size is smaller on nanowires compared to flat gallium phosphide. The cell area decreases with increasing the nanowire density on the substrate. We observed an effect of the nanowire diameter on MCF10A cells, with a decreased cell area on 40 nm diameter... (More)

Vertical nanowire arrays are increasingly investigated for their applications in steering cell behavior. The geometry of the array is an important parameter, which influences the morphology and adhesion of cells. Here, we investigate the effects of array geometry on the morphology of MCF7 cancer cells and MCF10A normal-like epithelial cells. Different gallium phosphide nanowire array-geometries were produced by varying the nanowire density and diameter. Our results show that the cell size is smaller on nanowires compared to flat gallium phosphide. The cell area decreases with increasing the nanowire density on the substrate. We observed an effect of the nanowire diameter on MCF10A cells, with a decreased cell area on 40 nm diameter nanowires, compared to 60 and 80 nm diameter nanowires in high-density arrays. The focal adhesion morphology depends on the extent to which cells are contacting the substrate. For low nanowire densities and diameters, cells are lying on the substrate and we observed large focal adhesions at the cell edges. In contrast, for high nanowire densities and diameters, cells are lying on top of the nanowires and we observed point-like focal adhesions distributed over the whole cell. Our results constitute a step towards the ability to fine-tune cell behavior on nanowire arrays.

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author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
aspect ratio, cell, density, diameter, morphology, nanowires
in
Science China Life Sciences
pages
9 pages
publisher
Science in China Press
external identifiers
  • scopus:85045289589
ISSN
1674-7305
DOI
10.1007/s11427-017-9264-2
language
English
LU publication?
yes
id
7bbf420e-066d-452a-b0f9-3ca262fcb91c
date added to LUP
2018-04-26 14:33:28
date last changed
2018-11-21 21:39:35
@article{7bbf420e-066d-452a-b0f9-3ca262fcb91c,
  abstract     = {<p>Vertical nanowire arrays are increasingly investigated for their applications in steering cell behavior. The geometry of the array is an important parameter, which influences the morphology and adhesion of cells. Here, we investigate the effects of array geometry on the morphology of MCF7 cancer cells and MCF10A normal-like epithelial cells. Different gallium phosphide nanowire array-geometries were produced by varying the nanowire density and diameter. Our results show that the cell size is smaller on nanowires compared to flat gallium phosphide. The cell area decreases with increasing the nanowire density on the substrate. We observed an effect of the nanowire diameter on MCF10A cells, with a decreased cell area on 40 nm diameter nanowires, compared to 60 and 80 nm diameter nanowires in high-density arrays. The focal adhesion morphology depends on the extent to which cells are contacting the substrate. For low nanowire densities and diameters, cells are lying on the substrate and we observed large focal adhesions at the cell edges. In contrast, for high nanowire densities and diameters, cells are lying on top of the nanowires and we observed point-like focal adhesions distributed over the whole cell. Our results constitute a step towards the ability to fine-tune cell behavior on nanowire arrays.</p>},
  author       = {Li, Zhen and Persson, Henrik and Adolfsson, Karl and Oredsson, Stina and Prinz, Christelle N.},
  issn         = {1674-7305},
  keyword      = {aspect ratio,cell,density,diameter,morphology,nanowires},
  language     = {eng},
  month        = {04},
  pages        = {1--9},
  publisher    = {Science in China Press},
  series       = {Science China Life Sciences},
  title        = {Morphology of living cells cultured on nanowire arrays with varying nanowire densities and diameters},
  url          = {http://dx.doi.org/10.1007/s11427-017-9264-2},
  year         = {2018},
}