A high-quality catalog of the Drosophila melanogaster proteome
(2007) In Nature Biotechnology 25(5). p.83-576- Abstract
Understanding how proteins and their complex interaction networks convert the genomic information into a dynamic living organism is a fundamental challenge in biological sciences. As an important step towards understanding the systems biology of a complex eukaryote, we cataloged 63% of the predicted Drosophila melanogaster proteome by detecting 9,124 proteins from 498,000 redundant and 72,281 distinct peptide identifications. This unprecedented high proteome coverage for a complex eukaryote was achieved by combining sample diversity, multidimensional biochemical fractionation and analysis-driven experimentation feedback loops, whereby data collection is guided by statistical analysis of prior data. We show that high-quality proteomics... (More)
Understanding how proteins and their complex interaction networks convert the genomic information into a dynamic living organism is a fundamental challenge in biological sciences. As an important step towards understanding the systems biology of a complex eukaryote, we cataloged 63% of the predicted Drosophila melanogaster proteome by detecting 9,124 proteins from 498,000 redundant and 72,281 distinct peptide identifications. This unprecedented high proteome coverage for a complex eukaryote was achieved by combining sample diversity, multidimensional biochemical fractionation and analysis-driven experimentation feedback loops, whereby data collection is guided by statistical analysis of prior data. We show that high-quality proteomics data provide crucial information to amend genome annotation and to confirm many predicted gene models. We also present experimentally identified proteotypic peptides matching approximately 50% of D. melanogaster gene models. This library of proteotypic peptides should enable fast, targeted and quantitative proteomic studies to elucidate the systems biology of this model organism.
(Less)
- author
- publishing date
- 2007-05
- type
- Contribution to journal
- publication status
- published
- keywords
- Amino Acid Sequence, Animals, Databases, Protein, Drosophila Proteins, Drosophila melanogaster, Molecular Sequence Data, Proteome, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't
- in
- Nature Biotechnology
- volume
- 25
- issue
- 5
- pages
- 8 pages
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:17450130
- scopus:34249717879
- ISSN
- 1087-0156
- DOI
- 10.1038/nbt1300
- language
- English
- LU publication?
- no
- id
- 7be523af-161c-474e-9fbe-2478570c9990
- date added to LUP
- 2016-11-16 20:40:35
- date last changed
- 2024-06-15 20:34:18
@article{7be523af-161c-474e-9fbe-2478570c9990, abstract = {{<p>Understanding how proteins and their complex interaction networks convert the genomic information into a dynamic living organism is a fundamental challenge in biological sciences. As an important step towards understanding the systems biology of a complex eukaryote, we cataloged 63% of the predicted Drosophila melanogaster proteome by detecting 9,124 proteins from 498,000 redundant and 72,281 distinct peptide identifications. This unprecedented high proteome coverage for a complex eukaryote was achieved by combining sample diversity, multidimensional biochemical fractionation and analysis-driven experimentation feedback loops, whereby data collection is guided by statistical analysis of prior data. We show that high-quality proteomics data provide crucial information to amend genome annotation and to confirm many predicted gene models. We also present experimentally identified proteotypic peptides matching approximately 50% of D. melanogaster gene models. This library of proteotypic peptides should enable fast, targeted and quantitative proteomic studies to elucidate the systems biology of this model organism.</p>}}, author = {{Brunner, Erich and Ahrens, Christian H and Mohanty, Sonali and Baetschmann, Hansruedi and Loevenich, Sandra and Potthast, Frank and Deutsch, Eric W and Panse, Christian and de Lichtenberg, Ulrik and Rinner, Oliver and Lee, Hookeun and Pedrioli, Patrick G A and Malmstrom, Johan and Koehler, Katja and Schrimpf, Sabine and Krijgsveld, Jeroen and Kregenow, Floyd and Heck, Albert J R and Hafen, Ernst and Schlapbach, Ralph and Aebersold, Ruedi}}, issn = {{1087-0156}}, keywords = {{Amino Acid Sequence; Animals; Databases, Protein; Drosophila Proteins; Drosophila melanogaster; Molecular Sequence Data; Proteome; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't}}, language = {{eng}}, number = {{5}}, pages = {{83--576}}, publisher = {{Nature Publishing Group}}, series = {{Nature Biotechnology}}, title = {{A high-quality catalog of the Drosophila melanogaster proteome}}, url = {{http://dx.doi.org/10.1038/nbt1300}}, doi = {{10.1038/nbt1300}}, volume = {{25}}, year = {{2007}}, }