Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Proteinase 3 and CD177 are expressed on the plasma membrane of the same subset of neutrophils.

Bauer, Susanne LU ; AbdGawad, Mohamed LU ; Gunnarsson, Lena LU ; Segelmark, Mårten LU ; Tapper, Hans LU and Hellmark, Thomas LU orcid (2007) In Journal of Leukocyte Biology 81. p.458-464
Abstract
Proteinase 3 (PR3) is found in granules of all nentrophils but also on the plasma membrane of a subset of nentrophils (mPR3). CD177, another neutrophil protein, also displays a bimodal surface expression. In this study, we have investigated the coexpression of these two molecules, as well as the effect of cell activation on their surface expression. We can show that CD177 is expressed on the same subset of nentrophils as mPR3. Experiments show that the expression of mPR3 and CD177 on the plasma membrane is increased or decreased in parallel during cell stimulation or spontaneous apoptosis. Furthermore, we observed a rapid internalization and recirculation of mPR3 and plasma membrane CD177, where A mPR3 is replaced within 30 min. Our... (More)
Proteinase 3 (PR3) is found in granules of all nentrophils but also on the plasma membrane of a subset of nentrophils (mPR3). CD177, another neutrophil protein, also displays a bimodal surface expression. In this study, we have investigated the coexpression of these two molecules, as well as the effect of cell activation on their surface expression. We can show that CD177 is expressed on the same subset of nentrophils as mPR3. Experiments show that the expression of mPR3 and CD177 on the plasma membrane is increased or decreased in parallel during cell stimulation or spontaneous apoptosis. Furthermore, we observed a rapid internalization and recirculation of mPR3 and plasma membrane CD177, where A mPR3 is replaced within 30 min. Our findings suggest that the PR3 found on the plasma membrane has its origin in the same intracellular storage as CD177, i.e., secondary granules and secretory vesicles and not primary granules. PR3- and CD177-expressing neutrophils constitute a subpopulation of neutrophils with an unknown role in the innate immune system, which may play an important role in diseases such as Wegener's granulomatosis and polycythemia vera. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
trafficking, serine proteases, Wegener's, granulomatosis, polycythemia vera, colocalization
in
Journal of Leukocyte Biology
volume
81
pages
458 - 464
publisher
John Wiley & Sons Inc.
external identifiers
  • wos:000243964700012
  • scopus:33846699482
  • pmid:17077162
ISSN
1938-3673
DOI
10.1189/jlb.0806514
language
English
LU publication?
yes
id
7c23580e-40b2-45d4-9cea-60a807a13302 (old id 163532)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17077162&dopt=Abstract
date added to LUP
2016-04-01 11:56:00
date last changed
2022-04-13 03:30:37
@article{7c23580e-40b2-45d4-9cea-60a807a13302,
  abstract     = {{Proteinase 3 (PR3) is found in granules of all nentrophils but also on the plasma membrane of a subset of nentrophils (mPR3). CD177, another neutrophil protein, also displays a bimodal surface expression. In this study, we have investigated the coexpression of these two molecules, as well as the effect of cell activation on their surface expression. We can show that CD177 is expressed on the same subset of nentrophils as mPR3. Experiments show that the expression of mPR3 and CD177 on the plasma membrane is increased or decreased in parallel during cell stimulation or spontaneous apoptosis. Furthermore, we observed a rapid internalization and recirculation of mPR3 and plasma membrane CD177, where A mPR3 is replaced within 30 min. Our findings suggest that the PR3 found on the plasma membrane has its origin in the same intracellular storage as CD177, i.e., secondary granules and secretory vesicles and not primary granules. PR3- and CD177-expressing neutrophils constitute a subpopulation of neutrophils with an unknown role in the innate immune system, which may play an important role in diseases such as Wegener's granulomatosis and polycythemia vera.}},
  author       = {{Bauer, Susanne and AbdGawad, Mohamed and Gunnarsson, Lena and Segelmark, Mårten and Tapper, Hans and Hellmark, Thomas}},
  issn         = {{1938-3673}},
  keywords     = {{trafficking; serine proteases; Wegener's; granulomatosis; polycythemia vera; colocalization}},
  language     = {{eng}},
  pages        = {{458--464}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Journal of Leukocyte Biology}},
  title        = {{Proteinase 3 and CD177 are expressed on the plasma membrane of the same subset of neutrophils.}},
  url          = {{http://dx.doi.org/10.1189/jlb.0806514}},
  doi          = {{10.1189/jlb.0806514}},
  volume       = {{81}},
  year         = {{2007}},
}