Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Human NUDT22 Is a UDP-Glucose/Galactose Hydrolase Exhibiting a Unique Structural Fold

Carter, Megan ; Jemth, Ann-Sofie ; Carreras-Puigvert, Jordi ; Herr, Patrick ; Martínez Carranza, Markel ; Vallin, Karl S A ; Throup, Adam ; Helleday, Thomas and Stenmark, Pål LU orcid (2018) In Structure 26(2). p.6-303
Abstract

Human NUDT22 belongs to the diverse NUDIX family of proteins, but has, until now, remained uncharacterized. Here we show that human NUDT22 is a Mg2+-dependent UDP-glucose and UDP-galactose hydrolase, producing UMP and glucose 1-phosphate or galactose 1-phosphate. We present the structure of human NUDT22 alone and in a complex with the substrate UDP-glucose. These structures reveal a partially conserved NUDIX fold domain preceded by a unique N-terminal domain responsible for UDP moiety binding and recognition. The NUDIX domain of NUDT22 contains a modified NUDIX box identified using structural analysis and confirmed through functional analysis of mutants. Human NUDT22's distinct structure and function as a UDP-carbohydrate hydrolase... (More)

Human NUDT22 belongs to the diverse NUDIX family of proteins, but has, until now, remained uncharacterized. Here we show that human NUDT22 is a Mg2+-dependent UDP-glucose and UDP-galactose hydrolase, producing UMP and glucose 1-phosphate or galactose 1-phosphate. We present the structure of human NUDT22 alone and in a complex with the substrate UDP-glucose. These structures reveal a partially conserved NUDIX fold domain preceded by a unique N-terminal domain responsible for UDP moiety binding and recognition. The NUDIX domain of NUDT22 contains a modified NUDIX box identified using structural analysis and confirmed through functional analysis of mutants. Human NUDT22's distinct structure and function as a UDP-carbohydrate hydrolase establish a unique NUDIX protein subfamily.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Galactosephosphates/metabolism, Glucosephosphates/metabolism, Humans, Phosphoric Diester Hydrolases/metabolism, Protein Folding
in
Structure
volume
26
issue
2
pages
6 - 303
publisher
Cell Press
external identifiers
  • scopus:85041395077
  • pmid:29413322
ISSN
0969-2126
DOI
10.1016/j.str.2018.01.004
language
English
LU publication?
no
additional info
Copyright © 2018 Elsevier Ltd. All rights reserved.
id
7c87bba9-4dd6-449c-bcf2-00c74f7c1851
date added to LUP
2019-04-30 07:49:21
date last changed
2024-04-02 02:13:55
@article{7c87bba9-4dd6-449c-bcf2-00c74f7c1851,
  abstract     = {{<p>Human NUDT22 belongs to the diverse NUDIX family of proteins, but has, until now, remained uncharacterized. Here we show that human NUDT22 is a Mg2+-dependent UDP-glucose and UDP-galactose hydrolase, producing UMP and glucose 1-phosphate or galactose 1-phosphate. We present the structure of human NUDT22 alone and in a complex with the substrate UDP-glucose. These structures reveal a partially conserved NUDIX fold domain preceded by a unique N-terminal domain responsible for UDP moiety binding and recognition. The NUDIX domain of NUDT22 contains a modified NUDIX box identified using structural analysis and confirmed through functional analysis of mutants. Human NUDT22's distinct structure and function as a UDP-carbohydrate hydrolase establish a unique NUDIX protein subfamily.</p>}},
  author       = {{Carter, Megan and Jemth, Ann-Sofie and Carreras-Puigvert, Jordi and Herr, Patrick and Martínez Carranza, Markel and Vallin, Karl S A and Throup, Adam and Helleday, Thomas and Stenmark, Pål}},
  issn         = {{0969-2126}},
  keywords     = {{Galactosephosphates/metabolism; Glucosephosphates/metabolism; Humans; Phosphoric Diester Hydrolases/metabolism; Protein Folding}},
  language     = {{eng}},
  month        = {{02}},
  number       = {{2}},
  pages        = {{6--303}},
  publisher    = {{Cell Press}},
  series       = {{Structure}},
  title        = {{Human NUDT22 Is a UDP-Glucose/Galactose Hydrolase Exhibiting a Unique Structural Fold}},
  url          = {{http://dx.doi.org/10.1016/j.str.2018.01.004}},
  doi          = {{10.1016/j.str.2018.01.004}},
  volume       = {{26}},
  year         = {{2018}},
}