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Ultra-High Frequency Ultrasound Imaging of Bowel Wall in Hirschsprung’s Disease—Correlation and Agreement Analyses of Histoanatomy

Hawez, Tebin LU ; Graneli, Christina LU ; Erlöv, Tobias LU ; Gottberg, Emilia ; Munoz Mitev, Rodrigo ; Hagelsteen, Kristine LU orcid ; Evertsson, Maria LU ; Jansson, Tomas LU ; Cinthio, Magnus LU and Stenström, Pernilla LU orcid (2023) In Diagnostics 13(8).
Abstract

Hirschsprung’s disease (HD) is characterized by aganglionosis in the bowel wall, requiring resection. Ultra-high frequency ultrasound (UHFUS) imaging of the bowel wall has been suggested to be an instantaneous method of deciding resection length. The aim of this study was to validate UHFUS imaging of the bowel wall in children with HD by exploring the correlation and systematic differences between UHFUS and histopathology. Resected fresh bowel specimens of children 0–1 years old, operated on for rectosigmoid aganglionosis at a national HD center 2018–2021, were examined ex vivo with UHFUS center frequency 50 MHz. Aganglionosis and ganglionosis were confirmed by histopathological staining and immunohistochemistry. Histoanatomical layers... (More)

Hirschsprung’s disease (HD) is characterized by aganglionosis in the bowel wall, requiring resection. Ultra-high frequency ultrasound (UHFUS) imaging of the bowel wall has been suggested to be an instantaneous method of deciding resection length. The aim of this study was to validate UHFUS imaging of the bowel wall in children with HD by exploring the correlation and systematic differences between UHFUS and histopathology. Resected fresh bowel specimens of children 0–1 years old, operated on for rectosigmoid aganglionosis at a national HD center 2018–2021, were examined ex vivo with UHFUS center frequency 50 MHz. Aganglionosis and ganglionosis were confirmed by histopathological staining and immunohistochemistry. Histoanatomical layers of bowel wall in histopathological and UHFUS images, respectively, were outlined using MATLAB programs. Both histopathological and UHFUS images were available for 19 aganglionic and 18 ganglionic specimens. The thickness of muscularis interna correlated positively between histopathology and UHFUS in both aganglionosis (R = 0.651, p = 0.003) and ganglionosis (R = 0.534, p = 0.023). The muscularis interna was systematically thicker in histopathology than in UHFUS images in both aganglionosis (0.499 vs. 0.309 mm; p < 0.001) and ganglionosis (0.644 versus 0.556 mm; p = 0.003). Significant correlations and systematic differences between histopathological and UHFUS images support the hypothesis that UHFUS reproduces the histoanatomy of the bowel wall in HD accurately.

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author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bowel wall, children, Hirschsprung’s disease, histopathology, ultra-high frequency ultrasound
in
Diagnostics
volume
13
issue
8
article number
1388
publisher
MDPI AG
external identifiers
  • pmid:37189490
  • scopus:85154614806
ISSN
2075-4418
DOI
10.3390/diagnostics13081388
language
English
LU publication?
yes
additional info
Publisher Copyright: © 2023 by the authors.
id
80068aae-2396-4b1b-8580-843715783835
date added to LUP
2023-07-28 21:30:11
date last changed
2024-04-19 23:59:34
@article{80068aae-2396-4b1b-8580-843715783835,
  abstract     = {{<p>Hirschsprung’s disease (HD) is characterized by aganglionosis in the bowel wall, requiring resection. Ultra-high frequency ultrasound (UHFUS) imaging of the bowel wall has been suggested to be an instantaneous method of deciding resection length. The aim of this study was to validate UHFUS imaging of the bowel wall in children with HD by exploring the correlation and systematic differences between UHFUS and histopathology. Resected fresh bowel specimens of children 0–1 years old, operated on for rectosigmoid aganglionosis at a national HD center 2018–2021, were examined ex vivo with UHFUS center frequency 50 MHz. Aganglionosis and ganglionosis were confirmed by histopathological staining and immunohistochemistry. Histoanatomical layers of bowel wall in histopathological and UHFUS images, respectively, were outlined using MATLAB programs. Both histopathological and UHFUS images were available for 19 aganglionic and 18 ganglionic specimens. The thickness of muscularis interna correlated positively between histopathology and UHFUS in both aganglionosis (R = 0.651, p = 0.003) and ganglionosis (R = 0.534, p = 0.023). The muscularis interna was systematically thicker in histopathology than in UHFUS images in both aganglionosis (0.499 vs. 0.309 mm; p &lt; 0.001) and ganglionosis (0.644 versus 0.556 mm; p = 0.003). Significant correlations and systematic differences between histopathological and UHFUS images support the hypothesis that UHFUS reproduces the histoanatomy of the bowel wall in HD accurately.</p>}},
  author       = {{Hawez, Tebin and Graneli, Christina and Erlöv, Tobias and Gottberg, Emilia and Munoz Mitev, Rodrigo and Hagelsteen, Kristine and Evertsson, Maria and Jansson, Tomas and Cinthio, Magnus and Stenström, Pernilla}},
  issn         = {{2075-4418}},
  keywords     = {{bowel wall; children; Hirschsprung’s disease; histopathology; ultra-high frequency ultrasound}},
  language     = {{eng}},
  number       = {{8}},
  publisher    = {{MDPI AG}},
  series       = {{Diagnostics}},
  title        = {{Ultra-High Frequency Ultrasound Imaging of Bowel Wall in Hirschsprung’s Disease—Correlation and Agreement Analyses of Histoanatomy}},
  url          = {{http://dx.doi.org/10.3390/diagnostics13081388}},
  doi          = {{10.3390/diagnostics13081388}},
  volume       = {{13}},
  year         = {{2023}},
}