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Confocal Raman microscopy for mapping phase segregation in individually dried particles composed of lactose and macromolecules

Nuzzo, Marine LU ; Sloth, Jakob; Brandner, Birgit; Bergenståhl, Björn LU and Millqvist-Fureby, Anna (2015) In Colloids and Surfaces A: Physicochemical and Engineering Aspects 481. p.229-236
Abstract
The quality of powder is determined by its functionality such as dissolution, encapsulation and flowability. The functionality of powder is in turn determined by their primary properties such as morphology and composition which need to be studied. Hence morphology and surface composition has been largely investigated in spray dried powders and individually dried particles. On the contrary, there is only scarce information regarding the internal structure. With the aim of acquiring a better understanding of the localization of different ingredients in spray dried powders we have used confocal Raman microscopy to investigate the internal microstructure of individually dried particles. In this study three different macromolecules have been... (More)
The quality of powder is determined by its functionality such as dissolution, encapsulation and flowability. The functionality of powder is in turn determined by their primary properties such as morphology and composition which need to be studied. Hence morphology and surface composition has been largely investigated in spray dried powders and individually dried particles. On the contrary, there is only scarce information regarding the internal structure. With the aim of acquiring a better understanding of the localization of different ingredients in spray dried powders we have used confocal Raman microscopy to investigate the internal microstructure of individually dried particles. In this study three different macromolecules have been investigated: bovine serum albumin, hydroxypropyl methyl cellulose, and triblock co-polymer poloxamer in a lactose matrix are compared at various macromolecule to lactose ratios. The surface and internal component distribution in response to the macromolecule concentration has been established. For the first time phase segregation in particles during a short drying time range is shown. Macromolecules were enriched at the surface of the dried particles and a macromolecule depleted layer was observed below the surface. Macromolecule enriched domains were found segregated from the amorphous lactose matrix in the internal part of the particles. Confocal Raman microscopy was found to be a powerful tool for internal mapping in individually dried particles. (C) 2015 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Confocal Raman microscopy, Phase segregation, Composition, Mapping, Individually dried particles
in
Colloids and Surfaces A: Physicochemical and Engineering Aspects
volume
481
pages
229 - 236
publisher
Elsevier
external identifiers
  • wos:000360407700028
  • scopus:84930683896
ISSN
0927-7757
DOI
10.1016/j.colsurfa.2015.04.044
language
English
LU publication?
yes
id
6699e885-4ed9-4ab8-8619-ca9796177897 (old id 8077479)
date added to LUP
2015-10-26 14:07:08
date last changed
2017-09-24 04:02:25
@article{6699e885-4ed9-4ab8-8619-ca9796177897,
  abstract     = {The quality of powder is determined by its functionality such as dissolution, encapsulation and flowability. The functionality of powder is in turn determined by their primary properties such as morphology and composition which need to be studied. Hence morphology and surface composition has been largely investigated in spray dried powders and individually dried particles. On the contrary, there is only scarce information regarding the internal structure. With the aim of acquiring a better understanding of the localization of different ingredients in spray dried powders we have used confocal Raman microscopy to investigate the internal microstructure of individually dried particles. In this study three different macromolecules have been investigated: bovine serum albumin, hydroxypropyl methyl cellulose, and triblock co-polymer poloxamer in a lactose matrix are compared at various macromolecule to lactose ratios. The surface and internal component distribution in response to the macromolecule concentration has been established. For the first time phase segregation in particles during a short drying time range is shown. Macromolecules were enriched at the surface of the dried particles and a macromolecule depleted layer was observed below the surface. Macromolecule enriched domains were found segregated from the amorphous lactose matrix in the internal part of the particles. Confocal Raman microscopy was found to be a powerful tool for internal mapping in individually dried particles. (C) 2015 Elsevier B.V. All rights reserved.},
  author       = {Nuzzo, Marine and Sloth, Jakob and Brandner, Birgit and Bergenståhl, Björn and Millqvist-Fureby, Anna},
  issn         = {0927-7757},
  keyword      = {Confocal Raman microscopy,Phase segregation,Composition,Mapping,Individually dried particles},
  language     = {eng},
  pages        = {229--236},
  publisher    = {Elsevier},
  series       = {Colloids and Surfaces A: Physicochemical and Engineering Aspects},
  title        = {Confocal Raman microscopy for mapping phase segregation in individually dried particles composed of lactose and macromolecules},
  url          = {http://dx.doi.org/10.1016/j.colsurfa.2015.04.044},
  volume       = {481},
  year         = {2015},
}