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Imaging dynamics of CD11c(+) cells and Foxp3(+) cells in progressive autoimmune insulitis in the NOD mouse model of type 1 diabetes

Schmidt-Christensen, Anja LU ; Hansen, Lisbeth LU ; Ilegems, Erwin; Fransén Pettersson, Nina LU ; Dahl, Ulf; Gupta, Shashank LU ; Larefalk, Asa; Hannibal, Tine LU ; Schulz, Alexander and Berggren, Per-Olof, et al. (2013) In Diabetologia 56(12). p.2669-2678
Abstract
The aim of this study was to visualise the dynamics and interactions of the cells involved in autoimmune-driven inflammation in type 1 diabetes. We adopted the anterior chamber of the eye (ACE) transplantation model to perform non-invasive imaging of leucocytes infiltrating the endocrine pancreas during initiation and progression of insulitis in the NOD mouse. Individual, ACE-transplanted islets of Langerhans were longitudinally and repetitively imaged by stereomicroscopy and two-photon microscopy to follow fluorescently labelled leucocyte subsets. We demonstrate that, in spite of the immune privileged status of the eye, the ACE-transplanted islets develop infiltration and beta cell destruction, recapitulating the autoimmune insulitis of... (More)
The aim of this study was to visualise the dynamics and interactions of the cells involved in autoimmune-driven inflammation in type 1 diabetes. We adopted the anterior chamber of the eye (ACE) transplantation model to perform non-invasive imaging of leucocytes infiltrating the endocrine pancreas during initiation and progression of insulitis in the NOD mouse. Individual, ACE-transplanted islets of Langerhans were longitudinally and repetitively imaged by stereomicroscopy and two-photon microscopy to follow fluorescently labelled leucocyte subsets. We demonstrate that, in spite of the immune privileged status of the eye, the ACE-transplanted islets develop infiltration and beta cell destruction, recapitulating the autoimmune insulitis of the pancreas, and exemplify this by analysing reporter cell populations expressing green fluorescent protein under the Cd11c or Foxp3 promoters. We also provide evidence that differences in morphological appearance of subpopulations of infiltrating leucocytes can be correlated to their distinct dynamic behaviour. Together, these findings demonstrate that the kinetics and dynamics of these key cellular components of autoimmune diabetes can be elucidated using this imaging platform for single cell resolution, non-invasive and repetitive monitoring of the individual islets of Langerhans during the natural development of autoimmune diabetes. (Less)
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publishing date
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Contribution to journal
publication status
published
subject
keywords
Animal-mouse, Imaging, Islet degeneration and damage, Islet, transplantation
in
Diabetologia
volume
56
issue
12
pages
2669 - 2678
publisher
Springer
external identifiers
  • wos:000326599300016
  • scopus:84887998165
ISSN
1432-0428
DOI
10.1007/s00125-013-3024-8
language
English
LU publication?
yes
id
80985fa6-8e4a-4b35-867f-cf814fb9af74 (old id 4204328)
date added to LUP
2014-01-03 10:45:39
date last changed
2019-06-19 01:18:50
@article{80985fa6-8e4a-4b35-867f-cf814fb9af74,
  abstract     = {The aim of this study was to visualise the dynamics and interactions of the cells involved in autoimmune-driven inflammation in type 1 diabetes. We adopted the anterior chamber of the eye (ACE) transplantation model to perform non-invasive imaging of leucocytes infiltrating the endocrine pancreas during initiation and progression of insulitis in the NOD mouse. Individual, ACE-transplanted islets of Langerhans were longitudinally and repetitively imaged by stereomicroscopy and two-photon microscopy to follow fluorescently labelled leucocyte subsets. We demonstrate that, in spite of the immune privileged status of the eye, the ACE-transplanted islets develop infiltration and beta cell destruction, recapitulating the autoimmune insulitis of the pancreas, and exemplify this by analysing reporter cell populations expressing green fluorescent protein under the Cd11c or Foxp3 promoters. We also provide evidence that differences in morphological appearance of subpopulations of infiltrating leucocytes can be correlated to their distinct dynamic behaviour. Together, these findings demonstrate that the kinetics and dynamics of these key cellular components of autoimmune diabetes can be elucidated using this imaging platform for single cell resolution, non-invasive and repetitive monitoring of the individual islets of Langerhans during the natural development of autoimmune diabetes.},
  author       = {Schmidt-Christensen, Anja and Hansen, Lisbeth and Ilegems, Erwin and Fransén Pettersson, Nina and Dahl, Ulf and Gupta, Shashank and Larefalk, Asa and Hannibal, Tine and Schulz, Alexander and Berggren, Per-Olof and Holmberg, Dan},
  issn         = {1432-0428},
  keyword      = {Animal-mouse,Imaging,Islet degeneration and damage,Islet,transplantation},
  language     = {eng},
  number       = {12},
  pages        = {2669--2678},
  publisher    = {Springer},
  series       = {Diabetologia},
  title        = {Imaging dynamics of CD11c(+) cells and Foxp3(+) cells in progressive autoimmune insulitis in the NOD mouse model of type 1 diabetes},
  url          = {http://dx.doi.org/10.1007/s00125-013-3024-8},
  volume       = {56},
  year         = {2013},
}