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Substrate and plant genotype strongly influence the growth and gene expression response to trichoderma afroharzianum T22 in sugar beet

Schmidt, John ; Dotson, Bradley R. LU ; Schmiderer, Ludwig LU ; van Tour, Adriaan ; Kumar, Banushree ; Marttila, Salla ; Fredlund, Kenneth M. ; Widell, Susanne LU and Rasmusson, Allan G. LU (2020) In Plants 9(8). p.1-14
Abstract

Many strains of Trichoderma fungi have beneficial effects on plant growth and pathogen control, but little is known about the importance of plant genotype, nor the underlying mechanisms. We aimed to determine the effect of sugar beet genotypic variation on Trichoderma biostimulation. The effect of Trichoderma afroharzianum T22 on sugar beet inbred genotypes were investigated in soil and on sterile agar medium regarding plant growth, and by quantitative reverse transcriptase-linked polymerase chain reaction (qRT-PCR) analysis for gene expression. In soil, T22 application induced up to 30% increase or decrease in biomass, depending on plant genotype. In contrast, T22 treatment of sterile-grown seedlings resulted in a general decrease in... (More)

Many strains of Trichoderma fungi have beneficial effects on plant growth and pathogen control, but little is known about the importance of plant genotype, nor the underlying mechanisms. We aimed to determine the effect of sugar beet genotypic variation on Trichoderma biostimulation. The effect of Trichoderma afroharzianum T22 on sugar beet inbred genotypes were investigated in soil and on sterile agar medium regarding plant growth, and by quantitative reverse transcriptase-linked polymerase chain reaction (qRT-PCR) analysis for gene expression. In soil, T22 application induced up to 30% increase or decrease in biomass, depending on plant genotype. In contrast, T22 treatment of sterile-grown seedlings resulted in a general decrease in fresh weight and root length across all sugar beet genotypes. Root colonization of T22 did not vary between the sugar beet genotypes. Sand-and sterile-grown roots were investigated by qRT-PCR for expression of marker genes for pathogen response pathways. Genotype-dependent effects of T22 on, especially, the jasmonic acid/ethylene expression marker PR3 were observed, and the effects were further dependent on the growth system used. Thus, both growth substrate and sugar beet genotype strongly affect the outcome of inoculation with T. afroharzianum T22.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Beta vulgaris, Gene expression, Growth stimulation, Inbred genotypes, Pathogen response pathways, Trichoderma afroharzianum
in
Plants
volume
9
issue
8
article number
1005
pages
14 pages
external identifiers
  • scopus:85090656416
  • pmid:32784636
ISSN
2223-7747
DOI
10.3390/plants9081005
language
English
LU publication?
yes
id
81cc91d5-6c1e-4a89-b3a3-f37152a519f1
date added to LUP
2020-10-21 15:50:12
date last changed
2021-01-06 10:08:19
@article{81cc91d5-6c1e-4a89-b3a3-f37152a519f1,
  abstract     = {<p>Many strains of Trichoderma fungi have beneficial effects on plant growth and pathogen control, but little is known about the importance of plant genotype, nor the underlying mechanisms. We aimed to determine the effect of sugar beet genotypic variation on Trichoderma biostimulation. The effect of Trichoderma afroharzianum T22 on sugar beet inbred genotypes were investigated in soil and on sterile agar medium regarding plant growth, and by quantitative reverse transcriptase-linked polymerase chain reaction (qRT-PCR) analysis for gene expression. In soil, T22 application induced up to 30% increase or decrease in biomass, depending on plant genotype. In contrast, T22 treatment of sterile-grown seedlings resulted in a general decrease in fresh weight and root length across all sugar beet genotypes. Root colonization of T22 did not vary between the sugar beet genotypes. Sand-and sterile-grown roots were investigated by qRT-PCR for expression of marker genes for pathogen response pathways. Genotype-dependent effects of T22 on, especially, the jasmonic acid/ethylene expression marker PR3 were observed, and the effects were further dependent on the growth system used. Thus, both growth substrate and sugar beet genotype strongly affect the outcome of inoculation with T. afroharzianum T22.</p>},
  author       = {Schmidt, John and Dotson, Bradley R. and Schmiderer, Ludwig and van Tour, Adriaan and Kumar, Banushree and Marttila, Salla and Fredlund, Kenneth M. and Widell, Susanne and Rasmusson, Allan G.},
  issn         = {2223-7747},
  language     = {eng},
  number       = {8},
  pages        = {1--14},
  series       = {Plants},
  title        = {Substrate and plant genotype strongly influence the growth and gene expression response to trichoderma afroharzianum T22 in sugar beet},
  url          = {http://dx.doi.org/10.3390/plants9081005},
  doi          = {10.3390/plants9081005},
  volume       = {9},
  year         = {2020},
}