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Cloning and sequence of a thermostable multidomain xylanase from the bacterium Rhodothermus marinus

Karlsson, Eva Nordberg LU orcid ; Bartonek-Roxå, Eva LU and Holst, Olle LU (1997) In Biochimica et Biophysica Acta - Gene Structure and Expression 1353(2). p.118-124
Abstract

The gene (xyn1) encoding a Rhodothermus marinus xylanase has been cloned and expressed in Escherichia coli. The gene comprises 5 different domains in an unusual combination. The cellulose binding domains (CBDs) encoded by xyn1 are repeated in tandem at the N-terminus and show similarity with the CBD family IV. The xyn1-gene is the first example encoding a CBD family IV in combination with a xylan hydrolyzing catalytic domain of the glycosyl hydrolase family 10.

Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
(Rhodothermus marinus), Cellulose binding domain family IV, Glycosyl hydrolase family 10, Xylanase
in
Biochimica et Biophysica Acta - Gene Structure and Expression
volume
1353
issue
2
pages
7 pages
publisher
Elsevier
external identifiers
  • pmid:9294005
  • scopus:0030800010
ISSN
0167-4781
DOI
10.1016/S0167-4781(97)00093-6
language
English
LU publication?
yes
id
8336abeb-7dfc-4348-b3f1-7314c8f2eaa9
date added to LUP
2018-11-14 21:14:29
date last changed
2025-10-16 07:00:33
@article{8336abeb-7dfc-4348-b3f1-7314c8f2eaa9,
  abstract     = {{<p>The gene (xyn1) encoding a Rhodothermus marinus xylanase has been cloned and expressed in Escherichia coli. The gene comprises 5 different domains in an unusual combination. The cellulose binding domains (CBDs) encoded by xyn1 are repeated in tandem at the N-terminus and show similarity with the CBD family IV. The xyn1-gene is the first example encoding a CBD family IV in combination with a xylan hydrolyzing catalytic domain of the glycosyl hydrolase family 10.</p>}},
  author       = {{Karlsson, Eva Nordberg and Bartonek-Roxå, Eva and Holst, Olle}},
  issn         = {{0167-4781}},
  keywords     = {{(Rhodothermus marinus); Cellulose binding domain family IV; Glycosyl hydrolase family 10; Xylanase}},
  language     = {{eng}},
  month        = {{08}},
  number       = {{2}},
  pages        = {{118--124}},
  publisher    = {{Elsevier}},
  series       = {{Biochimica et Biophysica Acta - Gene Structure and Expression}},
  title        = {{Cloning and sequence of a thermostable multidomain xylanase from the bacterium Rhodothermus marinus}},
  url          = {{http://dx.doi.org/10.1016/S0167-4781(97)00093-6}},
  doi          = {{10.1016/S0167-4781(97)00093-6}},
  volume       = {{1353}},
  year         = {{1997}},
}