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Acoustic trapping efficiency of nanoparticles and bacteria

Evander, Mikael LU ; Hammarström, Björn LU ; Ohlsson, Pelle LU ; Laurell, Thomas LU and Nilsson, Johan LU (2012) 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 p.515-517
Abstract

In this paper we present a method to characterize the acoustic trapping efficiency of nanoparticles and bacteria when using seeding particles. Through the use of fluorescent microscopy and video analysis, single particles/bacteria were counted as they entered the acoustic trap at different flow focusing ratios and by comparing the amount of trapped objects to the amount of objects that were lost from the trap, the trapping efficiency could be calculated. Using fluorescent 780 nm polystyrene particles, an optimization of the hydrodynamic sample pre-focusing could be performed. For a flow focusing ratio of 1:10 or 5:6 (sample:sheath flow), a trapping efficiency of around 90% could be achieved at a total flow rate of 11 μl/min. At a flow... (More)

In this paper we present a method to characterize the acoustic trapping efficiency of nanoparticles and bacteria when using seeding particles. Through the use of fluorescent microscopy and video analysis, single particles/bacteria were counted as they entered the acoustic trap at different flow focusing ratios and by comparing the amount of trapped objects to the amount of objects that were lost from the trap, the trapping efficiency could be calculated. Using fluorescent 780 nm polystyrene particles, an optimization of the hydrodynamic sample pre-focusing could be performed. For a flow focusing ratio of 1:10 or 5:6 (sample:sheath flow), a trapping efficiency of around 90% could be achieved at a total flow rate of 11 μl/min. At a flow focusing ratio of 1:10, GFP-producing E. coli could be trapped at an efficiency of above 95%. Using this characterization technique, important aspects of the acoustic trapping method (e.g. transducer frequency and voltage, size and type of seeding particle, amount of flow focusing, total flow rate etc.) can be characterized and optimized.

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Acoustic trapping, Bacteria, Nanoparticles, Trapping efficiency
host publication
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
pages
3 pages
publisher
Chemical and Biological Microsystems Society
conference name
16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
conference location
Okinawa, Japan
conference dates
2012-10-28 - 2012-11-01
external identifiers
  • scopus:84901778952
ISBN
9780979806452
language
English
LU publication?
yes
id
83c4af80-75f4-4a1c-8356-9eb8defb8ba0
date added to LUP
2017-12-06 15:16:09
date last changed
2019-01-06 13:35:50
@inproceedings{83c4af80-75f4-4a1c-8356-9eb8defb8ba0,
  abstract     = {<p>In this paper we present a method to characterize the acoustic trapping efficiency of nanoparticles and bacteria when using seeding particles. Through the use of fluorescent microscopy and video analysis, single particles/bacteria were counted as they entered the acoustic trap at different flow focusing ratios and by comparing the amount of trapped objects to the amount of objects that were lost from the trap, the trapping efficiency could be calculated. Using fluorescent 780 nm polystyrene particles, an optimization of the hydrodynamic sample pre-focusing could be performed. For a flow focusing ratio of 1:10 or 5:6 (sample:sheath flow), a trapping efficiency of around 90% could be achieved at a total flow rate of 11 μl/min. At a flow focusing ratio of 1:10, GFP-producing E. coli could be trapped at an efficiency of above 95%. Using this characterization technique, important aspects of the acoustic trapping method (e.g. transducer frequency and voltage, size and type of seeding particle, amount of flow focusing, total flow rate etc.) can be characterized and optimized.</p>},
  author       = {Evander, Mikael and Hammarström, Björn and Ohlsson, Pelle and Laurell, Thomas and Nilsson, Johan},
  isbn         = {9780979806452},
  keyword      = {Acoustic trapping,Bacteria,Nanoparticles,Trapping efficiency},
  language     = {eng},
  location     = {Okinawa, Japan},
  pages        = {515--517},
  publisher    = {Chemical and Biological Microsystems Society},
  title        = {Acoustic trapping efficiency of nanoparticles and bacteria},
  year         = {2012},
}