Acoustic trapping efficiency of nanoparticles and bacteria
Evander, Mikael LU ; Hammarström, Björn LU ; Ohlsson, Pelle LU
; Laurell, Thomas
LU
and Nilsson, Johan
LU
(2012)
16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
p.515-517
- Abstract
In this paper we present a method to characterize the acoustic trapping efficiency of nanoparticles and bacteria when using seeding particles. Through the use of fluorescent microscopy and video analysis, single particles/bacteria were counted as they entered the acoustic trap at different flow focusing ratios and by comparing the amount of trapped objects to the amount of objects that were lost from the trap, the trapping efficiency could be calculated. Using fluorescent 780 nm polystyrene particles, an optimization of the hydrodynamic sample pre-focusing could be performed. For a flow focusing ratio of 1:10 or 5:6 (sample:sheath flow), a trapping efficiency of around 90% could be achieved at a total flow rate of 11 μl/min. At a flow... (More)
In this paper we present a method to characterize the acoustic trapping efficiency of nanoparticles and bacteria when using seeding particles. Through the use of fluorescent microscopy and video analysis, single particles/bacteria were counted as they entered the acoustic trap at different flow focusing ratios and by comparing the amount of trapped objects to the amount of objects that were lost from the trap, the trapping efficiency could be calculated. Using fluorescent 780 nm polystyrene particles, an optimization of the hydrodynamic sample pre-focusing could be performed. For a flow focusing ratio of 1:10 or 5:6 (sample:sheath flow), a trapping efficiency of around 90% could be achieved at a total flow rate of 11 μl/min. At a flow focusing ratio of 1:10, GFP-producing E. coli could be trapped at an efficiency of above 95%. Using this characterization technique, important aspects of the acoustic trapping method (e.g. transducer frequency and voltage, size and type of seeding particle, amount of flow focusing, total flow rate etc.) can be characterized and optimized.
(Less)
- author
- Evander, Mikael
LU
; Hammarström, Björn
LU
; Ohlsson, Pelle
LU
; Laurell, Thomas
LU
and Nilsson, Johan
LU
- organization
- publishing date
- 2012
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Acoustic trapping, Bacteria, Nanoparticles, Trapping efficiency
- host publication
- Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
- pages
- 3 pages
- publisher
- Chemical and Biological Microsystems Society
- conference name
- 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
- conference location
- Okinawa, Japan
- conference dates
- 2012-10-28 - 2012-11-01
- external identifiers
-
- scopus:84901778952
- ISBN
- 9780979806452
- language
- English
- LU publication?
- yes
- id
- 83c4af80-75f4-4a1c-8356-9eb8defb8ba0
- date added to LUP
- 2017-12-06 15:16:09
- date last changed
- 2022-01-31 00:22:48
@inproceedings{83c4af80-75f4-4a1c-8356-9eb8defb8ba0,
abstract = {{<p>In this paper we present a method to characterize the acoustic trapping efficiency of nanoparticles and bacteria when using seeding particles. Through the use of fluorescent microscopy and video analysis, single particles/bacteria were counted as they entered the acoustic trap at different flow focusing ratios and by comparing the amount of trapped objects to the amount of objects that were lost from the trap, the trapping efficiency could be calculated. Using fluorescent 780 nm polystyrene particles, an optimization of the hydrodynamic sample pre-focusing could be performed. For a flow focusing ratio of 1:10 or 5:6 (sample:sheath flow), a trapping efficiency of around 90% could be achieved at a total flow rate of 11 μl/min. At a flow focusing ratio of 1:10, GFP-producing E. coli could be trapped at an efficiency of above 95%. Using this characterization technique, important aspects of the acoustic trapping method (e.g. transducer frequency and voltage, size and type of seeding particle, amount of flow focusing, total flow rate etc.) can be characterized and optimized.</p>}},
author = {{Evander, Mikael and Hammarström, Björn and Ohlsson, Pelle and Laurell, Thomas and Nilsson, Johan}},
booktitle = {{Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012}},
isbn = {{9780979806452}},
keywords = {{Acoustic trapping; Bacteria; Nanoparticles; Trapping efficiency}},
language = {{eng}},
pages = {{515--517}},
publisher = {{Chemical and Biological Microsystems Society}},
title = {{Acoustic trapping efficiency of nanoparticles and bacteria}},
year = {{2012}},
}