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Optimal Epinephrine Concentration and Time Delay to Minimize Perfusion in Eyelid Surgery : Measured by Laser-Based Methods and a Novel Form of Extended-Wavelength Diffuse Reflectance Spectroscopy

Sheikh, Rafi LU ; Dahlstrand, Ulf LU ; Memarzadeh, Khashayar LU ; Blohmé, Jonas LU ; Reistad, Nina LU and Malmsjö, Malin LU (2017) In Ophthalmic Plastic and Reconstructive Surgery
Abstract

OBJECTIVE:: This study investigates the hypoperfusion effects of epinephrine in local anesthesia in eyelid surgery. A novel form of extended-wavelength diffuse reflectance spectroscopy was evaluated. METHODS:: Blood perfusion in porcine eyelid flaps was measured using laser Doppler velocimetry and laser speckle contrast imaging, whereas the tissue response was measured using diffuse reflectance spectroscopy with a broad spectrum (450–1550 nm). Epinephrine was either injected cumulatively, 0.1 (1:10,000,000), 1.0 (1:1,000,000), 10 (1:100 000), and 100 μg/ml (1:10 000), to determine the dose–response relation, or given as a single dose (10 μg/ml). Control experiments were performed with saline or lidocaine. RESULTS:: Increasing... (More)

OBJECTIVE:: This study investigates the hypoperfusion effects of epinephrine in local anesthesia in eyelid surgery. A novel form of extended-wavelength diffuse reflectance spectroscopy was evaluated. METHODS:: Blood perfusion in porcine eyelid flaps was measured using laser Doppler velocimetry and laser speckle contrast imaging, whereas the tissue response was measured using diffuse reflectance spectroscopy with a broad spectrum (450–1550 nm). Epinephrine was either injected cumulatively, 0.1 (1:10,000,000), 1.0 (1:1,000,000), 10 (1:100 000), and 100 μg/ml (1:10 000), to determine the dose–response relation, or given as a single dose (10 μg/ml). Control experiments were performed with saline or lidocaine. RESULTS:: Increasing concentrations of epinephrine resulted in a gradual decrease in tissue perfusion, measured by laser Doppler velocimetry and laser speckle contrast imaging, approaching a minimum after the injection of 10 μg/ml. Similar tissue response was observed with diffuse reflectance spectroscopy. The time from the injection of epinephrine (10 μg/ml) to the stabilization of hypoperfusion was 75 seconds. After administration of 10 μg/ml epinephrine, about 20% of the blood perfusion remained, supporting the use of epinephrine in eyelid flaps with a narrow pedicle. CONCLUSIONS:: 10 μg/ml epinephrine appears to be adequate for vasoconstriction before oculoplastic surgery. Incisions need only be delayed for about 1 minute. Extended-wavelength diffuse reflectance spectroscopy appears to be a promising technique for monitoring the tissue response following changes in blood perfusion in plastic surgery reconstructions. However, more rigorous validation of the technique is required before it can be implemented in clinical practice.

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publication status
epub
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Ophthalmic Plastic and Reconstructive Surgery
publisher
Lippincott Williams and Wilkins
external identifiers
  • scopus:85013392557
ISSN
0740-9303
DOI
10.1097/IOP.0000000000000883
language
English
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yes
id
8447c6f6-1c46-40e0-a0b4-3d09995ec167
date added to LUP
2017-03-13 11:46:45
date last changed
2018-01-07 11:55:05
@article{8447c6f6-1c46-40e0-a0b4-3d09995ec167,
  abstract     = {<p>OBJECTIVE:: This study investigates the hypoperfusion effects of epinephrine in local anesthesia in eyelid surgery. A novel form of extended-wavelength diffuse reflectance spectroscopy was evaluated. METHODS:: Blood perfusion in porcine eyelid flaps was measured using laser Doppler velocimetry and laser speckle contrast imaging, whereas the tissue response was measured using diffuse reflectance spectroscopy with a broad spectrum (450–1550 nm). Epinephrine was either injected cumulatively, 0.1 (1:10,000,000), 1.0 (1:1,000,000), 10 (1:100 000), and 100 μg/ml (1:10 000), to determine the dose–response relation, or given as a single dose (10 μg/ml). Control experiments were performed with saline or lidocaine. RESULTS:: Increasing concentrations of epinephrine resulted in a gradual decrease in tissue perfusion, measured by laser Doppler velocimetry and laser speckle contrast imaging, approaching a minimum after the injection of 10 μg/ml. Similar tissue response was observed with diffuse reflectance spectroscopy. The time from the injection of epinephrine (10 μg/ml) to the stabilization of hypoperfusion was 75 seconds. After administration of 10 μg/ml epinephrine, about 20% of the blood perfusion remained, supporting the use of epinephrine in eyelid flaps with a narrow pedicle. CONCLUSIONS:: 10 μg/ml epinephrine appears to be adequate for vasoconstriction before oculoplastic surgery. Incisions need only be delayed for about 1 minute. Extended-wavelength diffuse reflectance spectroscopy appears to be a promising technique for monitoring the tissue response following changes in blood perfusion in plastic surgery reconstructions. However, more rigorous validation of the technique is required before it can be implemented in clinical practice.</p>},
  author       = {Sheikh, Rafi and Dahlstrand, Ulf and Memarzadeh, Khashayar and Blohmé, Jonas and Reistad, Nina and Malmsjö, Malin},
  issn         = {0740-9303},
  language     = {eng},
  month        = {02},
  publisher    = {Lippincott Williams and Wilkins},
  series       = {Ophthalmic Plastic and Reconstructive Surgery},
  title        = {Optimal Epinephrine Concentration and Time Delay to Minimize Perfusion in Eyelid Surgery : Measured by Laser-Based Methods and a Novel Form of Extended-Wavelength Diffuse Reflectance Spectroscopy},
  url          = {http://dx.doi.org/10.1097/IOP.0000000000000883},
  year         = {2017},
}