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Pressurized carbon dioxide as a potential tool for decellularization of pulmonary arteries for transplant purposes

Gil-Ramírez, Alicia LU ; Rosmark, Oskar LU ; Spégel, Peter LU ; Swärd, Karl LU ; Westergren-Thorsson, Gunilla LU ; Larsson-Callerfelt, Anna Karin LU and Rodríguez-Meizoso, Irene LU (2020) In Scientific Reports 10.
Abstract

Vascular bio-scaffolds produced from decellularized tissue offer a promising material for treatment of several types of cardiovascular diseases. These materials have the potential to maintain the functional properties of the extracellular matrix (ECM), and allow for growth and remodeling in vivo. The most commonly used methods for decellularization are based on chemicals and enzymes combinations, which often damage the ECM and cause cytotoxic effects in vivo. Mild methods involving pressurized CO2-ethanol (EtOH)-based fluids, in a supercritical or near supercritical state, have been studied for decellularization of cardiovascular tissue, but results are controversial. Moreover, data are lacking on the amount and type of... (More)

Vascular bio-scaffolds produced from decellularized tissue offer a promising material for treatment of several types of cardiovascular diseases. These materials have the potential to maintain the functional properties of the extracellular matrix (ECM), and allow for growth and remodeling in vivo. The most commonly used methods for decellularization are based on chemicals and enzymes combinations, which often damage the ECM and cause cytotoxic effects in vivo. Mild methods involving pressurized CO2-ethanol (EtOH)-based fluids, in a supercritical or near supercritical state, have been studied for decellularization of cardiovascular tissue, but results are controversial. Moreover, data are lacking on the amount and type of lipids remaining in the tissue. Here we show that pressurized CO2-EtOH-H2O fluids (average molar composition, ΧCO2 0.91) yielded close to complete removal of lipids from porcine pulmonary arteries, including a notably decrease of pro-inflammatory fatty acids. Pressurized CO2-limonene fluids (ΧCO2 0.88) and neat supercritical CO2 (scCO2) achieved the removal of 90% of triacylglycerides. Moreover, treatment of tissue with pressurized CO2-limonene followed by enzyme treatment, resulted in efficient DNA removal. The structure of elastic fibers was preserved after pressurized treatment, regardless solvent composition. In conclusion, pressurized CO2-ethanol fluids offer an efficient tool for delipidation in bio-scaffold production, while pressurized CO2-limonene fluids facilitate subsequent enzymatic removal of DNA.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
10
article number
4031
publisher
Nature Publishing Group
external identifiers
  • scopus:85081227678
  • pmid:32132596
ISSN
2045-2322
DOI
10.1038/s41598-020-60827-4
language
English
LU publication?
yes
id
84b864f7-8016-45c5-bfd2-0cd61310f629
date added to LUP
2020-03-26 17:12:36
date last changed
2020-03-27 03:00:03
@article{84b864f7-8016-45c5-bfd2-0cd61310f629,
  abstract     = {<p>Vascular bio-scaffolds produced from decellularized tissue offer a promising material for treatment of several types of cardiovascular diseases. These materials have the potential to maintain the functional properties of the extracellular matrix (ECM), and allow for growth and remodeling in vivo. The most commonly used methods for decellularization are based on chemicals and enzymes combinations, which often damage the ECM and cause cytotoxic effects in vivo. Mild methods involving pressurized CO<sub>2</sub>-ethanol (EtOH)-based fluids, in a supercritical or near supercritical state, have been studied for decellularization of cardiovascular tissue, but results are controversial. Moreover, data are lacking on the amount and type of lipids remaining in the tissue. Here we show that pressurized CO<sub>2</sub>-EtOH-H<sub>2</sub>O fluids (average molar composition, Χ<sub>CO2</sub> 0.91) yielded close to complete removal of lipids from porcine pulmonary arteries, including a notably decrease of pro-inflammatory fatty acids. Pressurized CO<sub>2</sub>-limonene fluids (Χ<sub>CO2</sub> 0.88) and neat supercritical CO<sub>2</sub> (scCO<sub>2</sub>) achieved the removal of 90% of triacylglycerides. Moreover, treatment of tissue with pressurized CO<sub>2</sub>-limonene followed by enzyme treatment, resulted in efficient DNA removal. The structure of elastic fibers was preserved after pressurized treatment, regardless solvent composition. In conclusion, pressurized CO<sub>2</sub>-ethanol fluids offer an efficient tool for delipidation in bio-scaffold production, while pressurized CO<sub>2</sub>-limonene fluids facilitate subsequent enzymatic removal of DNA.</p>},
  author       = {Gil-Ramírez, Alicia and Rosmark, Oskar and Spégel, Peter and Swärd, Karl and Westergren-Thorsson, Gunilla and Larsson-Callerfelt, Anna Karin and Rodríguez-Meizoso, Irene},
  issn         = {2045-2322},
  language     = {eng},
  month        = {03},
  publisher    = {Nature Publishing Group},
  series       = {Scientific Reports},
  title        = {Pressurized carbon dioxide as a potential tool for decellularization of pulmonary arteries for transplant purposes},
  url          = {http://dx.doi.org/10.1038/s41598-020-60827-4},
  doi          = {10.1038/s41598-020-60827-4},
  volume       = {10},
  year         = {2020},
}