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Prolonged life of human acute hippocampal slices from temporal lobe epilepsy surgery

Wickham, J. LU ; Brödjegård, N. G.; Vighagen, R.; Pinborg, L. H.; Bengzon, J. LU ; Woldbye, D. P.D.; Kokaia, M. LU and Andersson, M. LU (2018) In Scientific Reports 8(1).
Abstract

Resected hippocampal tissue from patients with drug-resistant epilepsy presents a unique possibility to test novel treatment strategies directly in target tissue. The post-resection time for testing and analysis however is normally limited. Acute tissue slices allow for electrophysiological recordings typically up to 12 hours. To enable longer time to test novel treatment strategies such as, e.g., gene-therapy, we developed a method for keeping acute human brain slices viable over a longer period. Our protocol keeps neurons viable well up to 48 hours. Using a dual-flow chamber, which allows for microscopic visualisation of individual neurons with a submerged objective for whole-cell patch-clamp recordings, we report stable... (More)

Resected hippocampal tissue from patients with drug-resistant epilepsy presents a unique possibility to test novel treatment strategies directly in target tissue. The post-resection time for testing and analysis however is normally limited. Acute tissue slices allow for electrophysiological recordings typically up to 12 hours. To enable longer time to test novel treatment strategies such as, e.g., gene-therapy, we developed a method for keeping acute human brain slices viable over a longer period. Our protocol keeps neurons viable well up to 48 hours. Using a dual-flow chamber, which allows for microscopic visualisation of individual neurons with a submerged objective for whole-cell patch-clamp recordings, we report stable electrophysiological properties, such as action potential amplitude and threshold during this time. We also demonstrate that epileptiform activity, monitored by individual dentate granule whole-cell recordings, can be consistently induced in these slices, underlying the usefulness of this methodology for testing and/or validating novel treatment strategies for epilepsy.

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organization
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type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
8
issue
1
publisher
Nature Publishing Group
external identifiers
  • scopus:85043262654
ISSN
2045-2322
DOI
10.1038/s41598-018-22554-9
language
English
LU publication?
yes
id
8500ada9-85fe-481f-9fd2-210e896fb769
date added to LUP
2018-03-19 14:26:50
date last changed
2018-10-25 21:46:20
@article{8500ada9-85fe-481f-9fd2-210e896fb769,
  abstract     = {<p>Resected hippocampal tissue from patients with drug-resistant epilepsy presents a unique possibility to test novel treatment strategies directly in target tissue. The post-resection time for testing and analysis however is normally limited. Acute tissue slices allow for electrophysiological recordings typically up to 12 hours. To enable longer time to test novel treatment strategies such as, e.g., gene-therapy, we developed a method for keeping acute human brain slices viable over a longer period. Our protocol keeps neurons viable well up to 48 hours. Using a dual-flow chamber, which allows for microscopic visualisation of individual neurons with a submerged objective for whole-cell patch-clamp recordings, we report stable electrophysiological properties, such as action potential amplitude and threshold during this time. We also demonstrate that epileptiform activity, monitored by individual dentate granule whole-cell recordings, can be consistently induced in these slices, underlying the usefulness of this methodology for testing and/or validating novel treatment strategies for epilepsy.</p>},
  articleno    = {4158},
  author       = {Wickham, J. and Brödjegård, N. G. and Vighagen, R. and Pinborg, L. H. and Bengzon, J. and Woldbye, D. P.D. and Kokaia, M. and Andersson, M.},
  issn         = {2045-2322},
  language     = {eng},
  month        = {12},
  number       = {1},
  publisher    = {Nature Publishing Group},
  series       = {Scientific Reports},
  title        = {Prolonged life of human acute hippocampal slices from temporal lobe epilepsy surgery},
  url          = {http://dx.doi.org/10.1038/s41598-018-22554-9},
  volume       = {8},
  year         = {2018},
}