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Defective alterations in the collagen network to prostacyclin in COPD lung fibroblasts

Larsson Callerfelt, Anna-Karin LU orcid ; Hallgren, Oskar LU ; Andersson Sjöland, Annika LU ; Thiman, Lena LU ; Bjorklund, Johan ; Kron, Josefine ; Nihlberg, Kristian LU ; Bjermer, Leif LU ; Löfdahl, Claes-Göran LU and Westergren-Thorsson, Gunilla LU orcid (2013) In Respiratory Research 14.
Abstract
Background: Prostacyclin analogs are potent vasodilators and possess anti-inflammatory properties. However, the effect of prostacyclin on extracellular matrix (ECM) in COPD is not well known. Collagen fibrils and proteoglycans are essential ECM components in the lung and fibroblasts are key players in regulating the homeostasis of ECM proteins. The aim was to study the synthesis of prostacyclin and its effect on fibroblast activity and ECM production, and in particular collagen I and the collagen-associated proteoglycans biglycan and decorin. Methods: Parenchymal lung fibroblasts were isolated from lungs from COPD patients (GOLD stage IV) and from lungs and transbronchial biopsies from control subjects. The prostacyclin analog iloprost was... (More)
Background: Prostacyclin analogs are potent vasodilators and possess anti-inflammatory properties. However, the effect of prostacyclin on extracellular matrix (ECM) in COPD is not well known. Collagen fibrils and proteoglycans are essential ECM components in the lung and fibroblasts are key players in regulating the homeostasis of ECM proteins. The aim was to study the synthesis of prostacyclin and its effect on fibroblast activity and ECM production, and in particular collagen I and the collagen-associated proteoglycans biglycan and decorin. Methods: Parenchymal lung fibroblasts were isolated from lungs from COPD patients (GOLD stage IV) and from lungs and transbronchial biopsies from control subjects. The prostacyclin analog iloprost was used to study the effect of prostacyclin on ECM protein synthesis, migration, proliferation and contractile capacity of fibroblasts. Results: TGF-beta(1) stimulation significantly increased prostacyclin synthesis in fibroblasts from COPD patients (p < 0.01), but showed no effect on fibroblasts from control subjects. Collagen I synthesis was decreased by iloprost in both control and COPD fibroblasts (p < 0.05). Conversely, iloprost significantly altered biglycan and decorin synthesis in control fibroblasts, but iloprost displayed no effect on these proteoglycans in COPD fibroblasts. Proliferation rate was reduced (p < 0.05) and contractile capacity was increased in COPD fibroblasts (p < 0.05) compared to control fibroblasts. Iloprost decreased proliferative rate in control fibroblasts (p < 0.05), whereas iloprost attenuated contraction capacity in both COPD (p < 0.01) and control fibroblasts (p < 0.05). Conclusions: Iloprost reduced collagen I synthesis and fibroblast contractility but did not affect the collagen-associated proteoglycans or proliferation rate in fibroblasts from COPD patients. Enhanced prostacyclin production could lead to improper collagen network fibrillogenesis and a more emphysematous lung structure in severe COPD patients. (Less)
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; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Chronic obstructive pulmonary disease, Collagen I, Fibroblast, Prostacyclin, Proteoglycans, Decorin, Biglycan, Proliferation, Fibroblast gel contraction, Transforming growth factor beta
in
Respiratory Research
volume
14
article number
21
publisher
BioMed Central (BMC)
external identifiers
  • wos:000315587100002
  • scopus:84873583925
  • pmid:23406566
ISSN
1465-9921
DOI
10.1186/1465-9921-14-21
language
English
LU publication?
yes
id
85fdf7ab-ecd0-4f09-89bc-d9382afb9b01 (old id 3671235)
date added to LUP
2016-04-01 10:00:28
date last changed
2024-05-19 04:44:15
@article{85fdf7ab-ecd0-4f09-89bc-d9382afb9b01,
  abstract     = {{Background: Prostacyclin analogs are potent vasodilators and possess anti-inflammatory properties. However, the effect of prostacyclin on extracellular matrix (ECM) in COPD is not well known. Collagen fibrils and proteoglycans are essential ECM components in the lung and fibroblasts are key players in regulating the homeostasis of ECM proteins. The aim was to study the synthesis of prostacyclin and its effect on fibroblast activity and ECM production, and in particular collagen I and the collagen-associated proteoglycans biglycan and decorin. Methods: Parenchymal lung fibroblasts were isolated from lungs from COPD patients (GOLD stage IV) and from lungs and transbronchial biopsies from control subjects. The prostacyclin analog iloprost was used to study the effect of prostacyclin on ECM protein synthesis, migration, proliferation and contractile capacity of fibroblasts. Results: TGF-beta(1) stimulation significantly increased prostacyclin synthesis in fibroblasts from COPD patients (p &lt; 0.01), but showed no effect on fibroblasts from control subjects. Collagen I synthesis was decreased by iloprost in both control and COPD fibroblasts (p &lt; 0.05). Conversely, iloprost significantly altered biglycan and decorin synthesis in control fibroblasts, but iloprost displayed no effect on these proteoglycans in COPD fibroblasts. Proliferation rate was reduced (p &lt; 0.05) and contractile capacity was increased in COPD fibroblasts (p &lt; 0.05) compared to control fibroblasts. Iloprost decreased proliferative rate in control fibroblasts (p &lt; 0.05), whereas iloprost attenuated contraction capacity in both COPD (p &lt; 0.01) and control fibroblasts (p &lt; 0.05). Conclusions: Iloprost reduced collagen I synthesis and fibroblast contractility but did not affect the collagen-associated proteoglycans or proliferation rate in fibroblasts from COPD patients. Enhanced prostacyclin production could lead to improper collagen network fibrillogenesis and a more emphysematous lung structure in severe COPD patients.}},
  author       = {{Larsson Callerfelt, Anna-Karin and Hallgren, Oskar and Andersson Sjöland, Annika and Thiman, Lena and Bjorklund, Johan and Kron, Josefine and Nihlberg, Kristian and Bjermer, Leif and Löfdahl, Claes-Göran and Westergren-Thorsson, Gunilla}},
  issn         = {{1465-9921}},
  keywords     = {{Chronic obstructive pulmonary disease; Collagen I; Fibroblast; Prostacyclin; Proteoglycans; Decorin; Biglycan; Proliferation; Fibroblast gel contraction; Transforming growth factor beta}},
  language     = {{eng}},
  publisher    = {{BioMed Central (BMC)}},
  series       = {{Respiratory Research}},
  title        = {{Defective alterations in the collagen network to prostacyclin in COPD lung fibroblasts}},
  url          = {{https://lup.lub.lu.se/search/files/1475205/4025375.pdf}},
  doi          = {{10.1186/1465-9921-14-21}},
  volume       = {{14}},
  year         = {{2013}},
}