A novel method for downstream characterization of breast cancer circulating tumor cells following CellSearch isolation.
Frithiof, Henrik LU ; Welinder, Charlotte LU ; Larsson, Anna-Maria LU ; Rydén, Lisa LU
and Aaltonen, Kristina
LU
(2015)
In Journal of Translational Medicine
13(1).
- Abstract
- Enumeration of circulating tumor cells (CTCs) obtained from minimally invasive blood samples has been well established as a valuable monitoring tool in metastatic and early breast cancer, as well as in several other cancer types. The gold standard technology for detecting CTCs in blood against a backdrop of millions of leukocytes is the FDA-approved CellSearch system (Janssen Diagnostics), which relies on EpCAM-based immunomagnetic separation. Secondary characterization of these cells could enable treatment selection based on specific targets in these cells, as well as providing a real time window into the metastatic process and offering unique insights into tumor heterogeneity. The objective of this study was to develop a method for... (More)
- Enumeration of circulating tumor cells (CTCs) obtained from minimally invasive blood samples has been well established as a valuable monitoring tool in metastatic and early breast cancer, as well as in several other cancer types. The gold standard technology for detecting CTCs in blood against a backdrop of millions of leukocytes is the FDA-approved CellSearch system (Janssen Diagnostics), which relies on EpCAM-based immunomagnetic separation. Secondary characterization of these cells could enable treatment selection based on specific targets in these cells, as well as providing a real time window into the metastatic process and offering unique insights into tumor heterogeneity. The objective of this study was to develop a method for downstream characterization of CTCs following isolation with the CellSearch system. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/5341114
- author
- Frithiof, Henrik
LU
; Welinder, Charlotte
LU
; Larsson, Anna-Maria
LU
; Rydén, Lisa
LU
and Aaltonen, Kristina
LU
- organization
-
- Breastcancer-genetics
- Tumor microenvironment
- Division of Translational Cancer Research
- The Liquid Biopsy and Tumor Progression in Breast Cancer (research group)
- Breast Cancer Surgery (research group)
- Surgery (Lund)
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Translational Medicine
- volume
- 13
- issue
- 1
- article number
- 126
- publisher
- BioMed Central (BMC)
- external identifiers
-
- pmid:25896421
- scopus:84928529608
- wos:000369792500001
- pmid:25896421
- ISSN
- 1479-5876
- DOI
- 10.1186/s12967-015-0493-1
- project
- CTC-MBC, Circulating Tumor Cells in Metastatic Breast Cancer
- Breast Cancer Surgery
- language
- English
- LU publication?
- yes
- id
- 8651c485-e00b-4260-841c-a25cc6ab14f3 (old id 5341114)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/25896421?dopt=Abstract
- date added to LUP
- 2016-04-01 14:00:21
- date last changed
- 2022-03-21 21:43:26
@article{8651c485-e00b-4260-841c-a25cc6ab14f3,
abstract = {{Enumeration of circulating tumor cells (CTCs) obtained from minimally invasive blood samples has been well established as a valuable monitoring tool in metastatic and early breast cancer, as well as in several other cancer types. The gold standard technology for detecting CTCs in blood against a backdrop of millions of leukocytes is the FDA-approved CellSearch system (Janssen Diagnostics), which relies on EpCAM-based immunomagnetic separation. Secondary characterization of these cells could enable treatment selection based on specific targets in these cells, as well as providing a real time window into the metastatic process and offering unique insights into tumor heterogeneity. The objective of this study was to develop a method for downstream characterization of CTCs following isolation with the CellSearch system.}},
author = {{Frithiof, Henrik and Welinder, Charlotte and Larsson, Anna-Maria and Rydén, Lisa and Aaltonen, Kristina}},
issn = {{1479-5876}},
language = {{eng}},
number = {{1}},
publisher = {{BioMed Central (BMC)}},
series = {{Journal of Translational Medicine}},
title = {{A novel method for downstream characterization of breast cancer circulating tumor cells following CellSearch isolation.}},
url = {{https://lup.lub.lu.se/search/files/3719349/8161007}},
doi = {{10.1186/s12967-015-0493-1}},
volume = {{13}},
year = {{2015}},
}