UV-induced changes in pigment content and light penetration in the fruticose lichen Cladonia arbuscula ssp. mitis
Buffoni Hall, Roberta LU ; Bornman, Janet LU and Björn, Lars Olof LU
(2002)
In Journal of Photochemistry and Photobiology, B: Biology
66(1).
p.13-20
- Abstract
- The response of the lichen, Cladonia arbuscula (Wallr.) Flot. ssp. mitis (Sandst.) Ruoss to enhanced UV-B (280-315 nm) radiation was investigated with respect to: (a) changes in phenolic content; (b) differential pigment accumulation under visible and UV radiation with increasing distance from thallus apices; and (c) the internal distribution of UV-B radiation within the thallus measured with quartz optical fibres. In a short-term experiment, lichens were exposed for 7 days in a growth chamber to visible light with or without additional UV-B radiation. For a longer term experiment, lichens were grown outdoors under both natural UV radiation, and supplemental UV-A (315–400 nm)+UV-B provided by lamps. Controls were placed under filters that... (More)
- The response of the lichen, Cladonia arbuscula (Wallr.) Flot. ssp. mitis (Sandst.) Ruoss to enhanced UV-B (280-315 nm) radiation was investigated with respect to: (a) changes in phenolic content; (b) differential pigment accumulation under visible and UV radiation with increasing distance from thallus apices; and (c) the internal distribution of UV-B radiation within the thallus measured with quartz optical fibres. In a short-term experiment, lichens were exposed for 7 days in a growth chamber to visible light with or without additional UV-B radiation. For a longer term experiment, lichens were grown outdoors under both natural UV radiation, and supplemental UV-A (315–400 nm)+UV-B provided by lamps. Controls were placed under filters that removed the radiation below 290 nm from the natural sunlight. The concentration of total phenolic compounds was measured spectrophotometrically at the termination of the experiments, in different parts of the lichen podetia. UV-exposed lichens showed increased accumulation of phenolics compared to those not grown under UV. At the termination of the long-term experiment, fibre optic measurements of the penetration of radiation into lichen thallus reflected the influence of growth under UV radiation, whereby UV was more strongly attenuated as compared to that in lichens not exposed to enhanced levels of UV-B radiation. Results indicated that in Cladonia, UV-B radiation induces the accumulation of phenolic compounds that may have a protective role. In addition, the morphological distribution of phenolic compounds was different under visible and supplemental UV-B radiation. Internal radiation measurements served to visualise the attenuation of radiation with thallus depth for different wavelengths in the UV-B waveband. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/134381
- author
- Buffoni Hall, Roberta
LU
; Bornman, Janet
LU
and Björn, Lars Olof
LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Photochemistry and Photobiology, B: Biology
- volume
- 66
- issue
- 1
- pages
- 13 - 20
- publisher
- Elsevier
- external identifiers
-
- wos:000174008300003
- scopus:0036471698
- ISSN
- 1011-1344
- DOI
- 10.1016/S1011-1344(01)00270-6
- project
- Photobiology
- language
- English
- LU publication?
- yes
- id
- 8662b006-05f6-4de6-82ea-4fae0f234be3 (old id 134381)
- date added to LUP
- 2016-04-01 16:32:44
- date last changed
- 2022-03-22 19:26:09
@article{8662b006-05f6-4de6-82ea-4fae0f234be3,
abstract = {{The response of the lichen, Cladonia arbuscula (Wallr.) Flot. ssp. mitis (Sandst.) Ruoss to enhanced UV-B (280-315 nm) radiation was investigated with respect to: (a) changes in phenolic content; (b) differential pigment accumulation under visible and UV radiation with increasing distance from thallus apices; and (c) the internal distribution of UV-B radiation within the thallus measured with quartz optical fibres. In a short-term experiment, lichens were exposed for 7 days in a growth chamber to visible light with or without additional UV-B radiation. For a longer term experiment, lichens were grown outdoors under both natural UV radiation, and supplemental UV-A (315–400 nm)+UV-B provided by lamps. Controls were placed under filters that removed the radiation below 290 nm from the natural sunlight. The concentration of total phenolic compounds was measured spectrophotometrically at the termination of the experiments, in different parts of the lichen podetia. UV-exposed lichens showed increased accumulation of phenolics compared to those not grown under UV. At the termination of the long-term experiment, fibre optic measurements of the penetration of radiation into lichen thallus reflected the influence of growth under UV radiation, whereby UV was more strongly attenuated as compared to that in lichens not exposed to enhanced levels of UV-B radiation. Results indicated that in Cladonia, UV-B radiation induces the accumulation of phenolic compounds that may have a protective role. In addition, the morphological distribution of phenolic compounds was different under visible and supplemental UV-B radiation. Internal radiation measurements served to visualise the attenuation of radiation with thallus depth for different wavelengths in the UV-B waveband.}},
author = {{Buffoni Hall, Roberta and Bornman, Janet and Björn, Lars Olof}},
issn = {{1011-1344}},
language = {{eng}},
number = {{1}},
pages = {{13--20}},
publisher = {{Elsevier}},
series = {{Journal of Photochemistry and Photobiology, B: Biology}},
title = {{UV-induced changes in pigment content and light penetration in the fruticose lichen Cladonia arbuscula ssp. mitis}},
url = {{http://dx.doi.org/10.1016/S1011-1344(01)00270-6}},
doi = {{10.1016/S1011-1344(01)00270-6}},
volume = {{66}},
year = {{2002}},
}