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Monitoring Nanostructure Dynamics and Polymerization in Glycerol Plasticized Wheat Gliadin and Glutenin Films : Relation to Mechanical Properties

Kuktaite, Ramune; Newson, William R.; Rasheed, Faiza; Plivelic, Tomás S. LU ; Hedenqvist, Mikael S.; Gällstedt, Mikael and Johansson, Eva (2016) In ACS Sustainable Chemistry and Engineering 4(6). p.2998-3007
Abstract

Gliadin and glutenin proteins with 10, 20, 30 and 40% of glycerol were compression molded into films (130 °C) and evaluated for protein polymerization, β-sheet structure and nano-structural morphology. Here, for the first time we show how different amounts of glycerol impact the nano-structure and functional properties of the gliadin and glutenin films. Most polymerized protein was found in the gliadin films with 20 and 30% glycerol, and in all the glutenin films (except 10%), by RP-HPLC. A β-sheet-rich protein structure was found to be high in the 10 and 20% glycerol gliadin films, and in the 20 and 30% glycerol glutenin films by FT-IR. Glycerol content of 20, 30 and 40% impacted the nano-structural morphology of the gliadin glycerol... (More)

Gliadin and glutenin proteins with 10, 20, 30 and 40% of glycerol were compression molded into films (130 °C) and evaluated for protein polymerization, β-sheet structure and nano-structural morphology. Here, for the first time we show how different amounts of glycerol impact the nano-structure and functional properties of the gliadin and glutenin films. Most polymerized protein was found in the gliadin films with 20 and 30% glycerol, and in all the glutenin films (except 10%), by RP-HPLC. A β-sheet-rich protein structure was found to be high in the 10 and 20% glycerol gliadin films, and in the 20 and 30% glycerol glutenin films by FT-IR. Glycerol content of 20, 30 and 40% impacted the nano-structural morphology of the gliadin glycerol films observed by SAXS, and to a limited extent for 10 and 20% glycerol gliadin films revealed by WAXS. No ordered nano-structure was found for the glutenin glycerol films. The 20%, 30% and 40% glycerol films were the most tunable for specific mechanical properties. For the highest stiffness and strength, the 10% glycerol protein films were the best choice.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Glycerol content, Polymerization, Protein film, Protein morphology, SAXS, WAXS
in
ACS Sustainable Chemistry and Engineering
volume
4
issue
6
pages
10 pages
publisher
The American Chemical Society
external identifiers
  • scopus:84973539342
  • wos:000377425900011
ISSN
2168-0485
DOI
10.1021/acssuschemeng.5b01667
language
English
LU publication?
yes
id
876f6845-998d-4f6a-9923-b64f17982649
date added to LUP
2017-01-24 12:48:09
date last changed
2017-09-18 11:33:37
@article{876f6845-998d-4f6a-9923-b64f17982649,
  abstract     = {<p>Gliadin and glutenin proteins with 10, 20, 30 and 40% of glycerol were compression molded into films (130 °C) and evaluated for protein polymerization, β-sheet structure and nano-structural morphology. Here, for the first time we show how different amounts of glycerol impact the nano-structure and functional properties of the gliadin and glutenin films. Most polymerized protein was found in the gliadin films with 20 and 30% glycerol, and in all the glutenin films (except 10%), by RP-HPLC. A β-sheet-rich protein structure was found to be high in the 10 and 20% glycerol gliadin films, and in the 20 and 30% glycerol glutenin films by FT-IR. Glycerol content of 20, 30 and 40% impacted the nano-structural morphology of the gliadin glycerol films observed by SAXS, and to a limited extent for 10 and 20% glycerol gliadin films revealed by WAXS. No ordered nano-structure was found for the glutenin glycerol films. The 20%, 30% and 40% glycerol films were the most tunable for specific mechanical properties. For the highest stiffness and strength, the 10% glycerol protein films were the best choice.</p>},
  author       = {Kuktaite, Ramune and Newson, William R. and Rasheed, Faiza and Plivelic, Tomás S. and Hedenqvist, Mikael S. and Gällstedt, Mikael and Johansson, Eva},
  issn         = {2168-0485},
  keyword      = {Glycerol content,Polymerization,Protein film,Protein morphology,SAXS,WAXS},
  language     = {eng},
  month        = {06},
  number       = {6},
  pages        = {2998--3007},
  publisher    = {The American Chemical Society},
  series       = {ACS Sustainable Chemistry and Engineering},
  title        = {Monitoring Nanostructure Dynamics and Polymerization in Glycerol Plasticized Wheat Gliadin and Glutenin Films : Relation to Mechanical Properties},
  url          = {http://dx.doi.org/10.1021/acssuschemeng.5b01667},
  volume       = {4},
  year         = {2016},
}