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Evaluation of sequential processing for the extraction of lipids, starch and proteins from wheat bran

Sardari, Roya LU ; Sutiono, Samuel ; Azeem, Hafiz A LU ; Galbe, Mats LU ; Larsson, Mats ; Turner, Charlotta LU orcid and Nordberg Karlsson, Eva LU orcid (2019) In Frontiers in Bioengineering and Biotechnology 7(413).
Abstract
In line with the need to better utilize agricultural resources, and valorize underutilized fractions, we have developed protocols to increase the use of wheat bran, to improve utilization of this resource to additional products. Here, we report sequential processing for extraction of starch, lipids, and proteins from wheat brans with two different particle sizes leaving a rest-material enriched in dietary fiber. Mild water-based extraction of starch resulted in maximum 81.7 ± 0.67% yield. Supercritical fluid extraction of lipids by CO2 resulted in 55.2 ± 2.4% yield. This was lower than the corresponding yield using Soxhlet extraction, which was used as a reference method, but allowed a continued extraction sequence without denaturation of... (More)
In line with the need to better utilize agricultural resources, and valorize underutilized fractions, we have developed protocols to increase the use of wheat bran, to improve utilization of this resource to additional products. Here, we report sequential processing for extraction of starch, lipids, and proteins from wheat brans with two different particle sizes leaving a rest-material enriched in dietary fiber. Mild water-based extraction of starch resulted in maximum 81.7 ± 0.67% yield. Supercritical fluid extraction of lipids by CO2 resulted in 55.2 ± 2.4% yield. This was lower than the corresponding yield using Soxhlet extraction, which was used as a reference method, but allowed a continued extraction sequence without denaturation of the proteins remaining in the raw-material. Alkaline extraction of non-degraded proteins resulted in a yield corresponding to one third of the total protein in the material, which was improved to reach 62 ± 8% by a combination of wheat bran enzymes activation followed by Osborne fractionation. The remaining proteins were extracted in degraded form, resulting in maximum 91.6 ± 1.6% yield of the total proteins content. The remaining material in both fine and coarse bran had a fiber content that on average corresponded to 73 ± 3%. The current work allows separation of several compounds, which is enabling valorization of the bran raw-material into several products. (Less)
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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Frontiers in Bioengineering and Biotechnology
volume
7
issue
413
publisher
Frontiers Media S. A.
external identifiers
  • scopus:85077339115
  • pmid:31921817
ISSN
2296-4185
DOI
10.3389/fbioe.2019.00413
language
English
LU publication?
yes
id
87e44f9f-397a-4bfd-b0be-8d18ee4f96d3
date added to LUP
2019-11-30 18:02:08
date last changed
2024-04-17 00:47:07
@article{87e44f9f-397a-4bfd-b0be-8d18ee4f96d3,
  abstract     = {{In line with the need to better utilize agricultural resources, and valorize underutilized fractions, we have developed protocols to increase the use of wheat bran, to improve utilization of this resource to additional products. Here, we report sequential processing for extraction of starch, lipids, and proteins from wheat brans with two different particle sizes leaving a rest-material enriched in dietary fiber. Mild water-based extraction of starch resulted in maximum 81.7 ± 0.67% yield. Supercritical fluid extraction of lipids by CO2 resulted in 55.2 ± 2.4% yield. This was lower than the corresponding yield using Soxhlet extraction, which was used as a reference method, but allowed a continued extraction sequence without denaturation of the proteins remaining in the raw-material. Alkaline extraction of non-degraded proteins resulted in a yield corresponding to one third of the total protein in the material, which was improved to reach 62 ± 8% by a combination of wheat bran enzymes activation followed by Osborne fractionation. The remaining proteins were extracted in degraded form, resulting in maximum 91.6 ± 1.6% yield of the total proteins content. The remaining material in both fine and coarse bran had a fiber content that on average corresponded to 73 ± 3%. The current work allows separation of several compounds, which is enabling valorization of the bran raw-material into several products.}},
  author       = {{Sardari, Roya and Sutiono, Samuel and Azeem, Hafiz A and Galbe, Mats and Larsson, Mats and Turner, Charlotta and Nordberg Karlsson, Eva}},
  issn         = {{2296-4185}},
  language     = {{eng}},
  month        = {{11}},
  number       = {{413}},
  publisher    = {{Frontiers Media S. A.}},
  series       = {{Frontiers in Bioengineering and Biotechnology}},
  title        = {{Evaluation of sequential processing for the extraction of lipids, starch and proteins from wheat bran}},
  url          = {{http://dx.doi.org/10.3389/fbioe.2019.00413}},
  doi          = {{10.3389/fbioe.2019.00413}},
  volume       = {{7}},
  year         = {{2019}},
}