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Electron microscopy imaging of proteins on gallium phosphide semiconductor nanowires

Hjort, Martin LU orcid ; Bauer, Mikael LU ; Gunnarsson, Stefan LU ; Mårsell, Erik LU ; Zakharov, Alexei LU ; Karlsson, Gunnel LU ; Sanfins, Elodie LU ; Prinz, Christelle LU ; Wallenberg, Reine LU and Cedervall, Tommy LU , et al. (2016) In Nanoscale 8(7). p.3936-3943
Abstract

We have imaged GaP nanowires (NWs) incubated with human laminin, serum albumin (HSA), and blood plasma using both cryo-transmission electron microscopy and synchrotron based X-ray photoemission electron microscopy. This extensive imaging methodology simultaneously reveals structural, chemical and morphological details of individual nanowires and the adsorbed proteins. We found that the proteins bind to NWs, forming coronas with thicknesses close to the proteins' hydrodynamic diameters. We could directly image how laminin is extending from the NWs, maximizing the number of proteins bound to the NWs. NWs incubated with both laminin and HSA show protein coronas with a similar appearance to NWs incubated with laminin alone, indicating that... (More)

We have imaged GaP nanowires (NWs) incubated with human laminin, serum albumin (HSA), and blood plasma using both cryo-transmission electron microscopy and synchrotron based X-ray photoemission electron microscopy. This extensive imaging methodology simultaneously reveals structural, chemical and morphological details of individual nanowires and the adsorbed proteins. We found that the proteins bind to NWs, forming coronas with thicknesses close to the proteins' hydrodynamic diameters. We could directly image how laminin is extending from the NWs, maximizing the number of proteins bound to the NWs. NWs incubated with both laminin and HSA show protein coronas with a similar appearance to NWs incubated with laminin alone, indicating that the presence of HSA does not affect the laminin conformation on the NWs. In blood plasma, an intermediate sized corona around the NWs indicates a corona with a mixture of plasma proteins. The ability to directly visualize proteins on nanostructures in situ holds great promise for assessing the conformation and thickness of the protein corona, which is key to understanding and predicting the properties of engineered nanomaterials in a biological environment.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Nanoscale
volume
8
issue
7
pages
3936 - 3943
publisher
Royal Society of Chemistry
external identifiers
  • pmid:26838122
  • scopus:84958568458
  • wos:000370761700010
  • pmid:26838122
ISSN
2040-3372
DOI
10.1039/c5nr08888g
language
English
LU publication?
yes
id
f3d379af-7ae5-4804-b3a7-bbd288a85f38 (old id 8829451)
date added to LUP
2016-04-01 10:00:44
date last changed
2025-04-04 15:20:52
@article{f3d379af-7ae5-4804-b3a7-bbd288a85f38,
  abstract     = {{<p>We have imaged GaP nanowires (NWs) incubated with human laminin, serum albumin (HSA), and blood plasma using both cryo-transmission electron microscopy and synchrotron based X-ray photoemission electron microscopy. This extensive imaging methodology simultaneously reveals structural, chemical and morphological details of individual nanowires and the adsorbed proteins. We found that the proteins bind to NWs, forming coronas with thicknesses close to the proteins' hydrodynamic diameters. We could directly image how laminin is extending from the NWs, maximizing the number of proteins bound to the NWs. NWs incubated with both laminin and HSA show protein coronas with a similar appearance to NWs incubated with laminin alone, indicating that the presence of HSA does not affect the laminin conformation on the NWs. In blood plasma, an intermediate sized corona around the NWs indicates a corona with a mixture of plasma proteins. The ability to directly visualize proteins on nanostructures in situ holds great promise for assessing the conformation and thickness of the protein corona, which is key to understanding and predicting the properties of engineered nanomaterials in a biological environment.</p>}},
  author       = {{Hjort, Martin and Bauer, Mikael and Gunnarsson, Stefan and Mårsell, Erik and Zakharov, Alexei and Karlsson, Gunnel and Sanfins, Elodie and Prinz, Christelle and Wallenberg, Reine and Cedervall, Tommy and Mikkelsen, Anders}},
  issn         = {{2040-3372}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{3936--3943}},
  publisher    = {{Royal Society of Chemistry}},
  series       = {{Nanoscale}},
  title        = {{Electron microscopy imaging of proteins on gallium phosphide semiconductor nanowires}},
  url          = {{http://dx.doi.org/10.1039/c5nr08888g}},
  doi          = {{10.1039/c5nr08888g}},
  volume       = {{8}},
  year         = {{2016}},
}