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Preparation of Decellularized Lung Matrices for Cell Culture and Protein Analysis

Uhl, Franziska E; Wagner, Darcy E LU and Weiss, Daniel J. (2017) In Methods in Molecular Biology 1627. p.253-283
Abstract

The limited available treatment options for patients with chronic lung diseases, such as fibrosis, lead to poor prognosis after diagnosis and short survival rates. An exciting new bioengineering approach utilizes de- and recellularization of lung tissue to potentially overcome donor organ shortage and immune reactions toward the received transplant. The goal of decellularization is to create a scaffold which contains the necessary framework for stability and functionality for regenerating lung tissue while removing immunomodulatory factors by removal of cells. After decellularization, the scaffold could be re-functionalized by repopulation with the patient's own stem/progenitor cells to create a fully functional organ or can be used as... (More)

The limited available treatment options for patients with chronic lung diseases, such as fibrosis, lead to poor prognosis after diagnosis and short survival rates. An exciting new bioengineering approach utilizes de- and recellularization of lung tissue to potentially overcome donor organ shortage and immune reactions toward the received transplant. The goal of decellularization is to create a scaffold which contains the necessary framework for stability and functionality for regenerating lung tissue while removing immunomodulatory factors by removal of cells. After decellularization, the scaffold could be re-functionalized by repopulation with the patient's own stem/progenitor cells to create a fully functional organ or can be used as ex vivo models of disease. In this chapter the decellularization of lung tissue from multiple species (i.e., rodents, pigs, and humans) as well as disease states such as fibrosis is described. We discuss and describe the various quality control measures which should be used to characterize decellularized scaffolds, methods for protein analysis of the remaining scaffold, and methods for recellularization of scaffolds.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Journal Article
in
Methods in Molecular Biology
volume
1627
pages
253 - 283
publisher
Springer
external identifiers
  • scopus:85028451235
ISSN
1940-6029
DOI
10.1007/978-1-4939-7113-8_18
language
English
LU publication?
no
id
885e6e8a-e958-46c4-9141-d6ee0ec91873
date added to LUP
2017-09-18 15:19:15
date last changed
2018-01-07 12:18:55
@article{885e6e8a-e958-46c4-9141-d6ee0ec91873,
  abstract     = {<p>The limited available treatment options for patients with chronic lung diseases, such as fibrosis, lead to poor prognosis after diagnosis and short survival rates. An exciting new bioengineering approach utilizes de- and recellularization of lung tissue to potentially overcome donor organ shortage and immune reactions toward the received transplant. The goal of decellularization is to create a scaffold which contains the necessary framework for stability and functionality for regenerating lung tissue while removing immunomodulatory factors by removal of cells. After decellularization, the scaffold could be re-functionalized by repopulation with the patient's own stem/progenitor cells to create a fully functional organ or can be used as ex vivo models of disease. In this chapter the decellularization of lung tissue from multiple species (i.e., rodents, pigs, and humans) as well as disease states such as fibrosis is described. We discuss and describe the various quality control measures which should be used to characterize decellularized scaffolds, methods for protein analysis of the remaining scaffold, and methods for recellularization of scaffolds.</p>},
  author       = {Uhl, Franziska E and Wagner, Darcy E and Weiss, Daniel J.},
  issn         = {1940-6029},
  keyword      = {Journal Article},
  language     = {eng},
  pages        = {253--283},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Preparation of Decellularized Lung Matrices for Cell Culture and Protein Analysis},
  url          = {http://dx.doi.org/10.1007/978-1-4939-7113-8_18},
  volume       = {1627},
  year         = {2017},
}