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A 50 µm acoustic resonator microchannel enables focusing 100 nm polystyrene beads and sub-micron bioparticles

Tsuyama, Yoshiyuki ; Xu, Bin ; Hattori, Kazuki ; Baek, Seugho ; Yoshioka, Yusuke ; Kojima, Ryosuke ; Cho, Younghak ; Laurell, Thomas LU ; Kim, Soyoun and Ota, Sadao , et al. (2023) In Sensors and Actuators B: Chemical 376.
Abstract

The manipulation and focusing of submicron/nanometre-scale objects are becoming increasingly important in the fields of biology, chemistry, and materials science. Acoustofluidics provides for the high throughput and precise manipulation of cells and particles with simple equipment. However, application to submicron/nanoparticles is challenging due to the particle volume dependence of the acoustic radiation force and the relatively larger effect of the viscous drag force on small objects. Here, we present an acoustofluidic device that can focus submicron/nanoparticles, bacterial cells, and extracellular vesicles (EVs) using a high-frequency two-dimensional acoustic standing wave. We fabricate 50 µm × 50 µm square cross-section channels... (More)

The manipulation and focusing of submicron/nanometre-scale objects are becoming increasingly important in the fields of biology, chemistry, and materials science. Acoustofluidics provides for the high throughput and precise manipulation of cells and particles with simple equipment. However, application to submicron/nanoparticles is challenging due to the particle volume dependence of the acoustic radiation force and the relatively larger effect of the viscous drag force on small objects. Here, we present an acoustofluidic device that can focus submicron/nanoparticles, bacterial cells, and extracellular vesicles (EVs) using a high-frequency two-dimensional acoustic standing wave. We fabricate 50 µm × 50 µm square cross-section channels as acoustic resonators and generate a two-dimensional acoustic standing wave at an actuation frequency of 14.9 MHz. This configuration enables a strong acoustic radiation force towards the centre of the microchannel and a cross-section-centred single-vortex stream that does not counteract the acoustic radiation force. Using this device, polystyrene beads ranging in diameter from 50 to 500 nm were focused toward the channel centre, where a focused bead stream width of less than 5 µm was achieved at a flow rate of 3 µL min−1 for 100 nm beads and 9 µL min−1 for 200 nm beads. Furthermore, bacterial cells were successfully focused at a flow rate of 40 µL min−1, and the focusing of EVs was also demonstrated. Our acoustofluidic device can become a promising tool for a wide range of biological analyses targeting submicron cells, viruses, and EVs.

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organization
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type
Contribution to journal
publication status
published
subject
keywords
Acoustofluidics, Bulk acoustic wave (BAW), Extracellular Vesicles (EVs), Flow cytometry, Nanoparticle focusing
in
Sensors and Actuators B: Chemical
volume
376
article number
132918
publisher
Elsevier
external identifiers
  • scopus:85142205927
ISSN
0925-4005
DOI
10.1016/j.snb.2022.132918
language
English
LU publication?
yes
id
8af53fa2-5352-4c33-a2d1-a388edf93bec
date added to LUP
2023-02-09 14:05:39
date last changed
2024-05-17 10:11:54
@article{8af53fa2-5352-4c33-a2d1-a388edf93bec,
  abstract     = {{<p>The manipulation and focusing of submicron/nanometre-scale objects are becoming increasingly important in the fields of biology, chemistry, and materials science. Acoustofluidics provides for the high throughput and precise manipulation of cells and particles with simple equipment. However, application to submicron/nanoparticles is challenging due to the particle volume dependence of the acoustic radiation force and the relatively larger effect of the viscous drag force on small objects. Here, we present an acoustofluidic device that can focus submicron/nanoparticles, bacterial cells, and extracellular vesicles (EVs) using a high-frequency two-dimensional acoustic standing wave. We fabricate 50 µm × 50 µm square cross-section channels as acoustic resonators and generate a two-dimensional acoustic standing wave at an actuation frequency of 14.9 MHz. This configuration enables a strong acoustic radiation force towards the centre of the microchannel and a cross-section-centred single-vortex stream that does not counteract the acoustic radiation force. Using this device, polystyrene beads ranging in diameter from 50 to 500 nm were focused toward the channel centre, where a focused bead stream width of less than 5 µm was achieved at a flow rate of 3 µL min<sup>−1</sup> for 100 nm beads and 9 µL min<sup>−1</sup> for 200 nm beads. Furthermore, bacterial cells were successfully focused at a flow rate of 40 µL min<sup>−1</sup>, and the focusing of EVs was also demonstrated. Our acoustofluidic device can become a promising tool for a wide range of biological analyses targeting submicron cells, viruses, and EVs.</p>}},
  author       = {{Tsuyama, Yoshiyuki and Xu, Bin and Hattori, Kazuki and Baek, Seugho and Yoshioka, Yusuke and Kojima, Ryosuke and Cho, Younghak and Laurell, Thomas and Kim, Soyoun and Ota, Sadao and Lee, Sang Wook}},
  issn         = {{0925-4005}},
  keywords     = {{Acoustofluidics; Bulk acoustic wave (BAW); Extracellular Vesicles (EVs); Flow cytometry; Nanoparticle focusing}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Sensors and Actuators B: Chemical}},
  title        = {{A 50 µm acoustic resonator microchannel enables focusing 100 nm polystyrene beads and sub-micron bioparticles}},
  url          = {{http://dx.doi.org/10.1016/j.snb.2022.132918}},
  doi          = {{10.1016/j.snb.2022.132918}},
  volume       = {{376}},
  year         = {{2023}},
}