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Effects of glomerular filtration rate on Ficoll sieving coefficients (theta) in rats.

Rippe, Catarina LU ; Asgeirsson, Daniel LU ; Venturoli, Daniele LU ; Rippe, Anna LU and Rippe, Bengt LU (2006) In Kidney International 69(8). p.1326-1332
Abstract
The purpose of the present study was to assess the role of diffusion and convection during filtration of Ficoll across the glomerular filter by comparing glomerular sieving coefficients ( h) to neutral fluorescein isothiocyanate ( FITC)-Ficoll 70/400 obtained at low ( hydropenic) vs raised ( normal) glomerular filtration rates (GFRs). The h for FITC-Ficoll was determined in anesthetized Wistar rats (304 +/- 18 g) following laparotomy and cannulation of the ureters, used for urine sampling. After surgery, GFR was 1.2 +/- 0.16 ml/ min (+/- s. e.), assessed using the plasma to urine clearance of FITC-inulin and Cr-51-ethylenediaminetetraacetic acid. FITC-Ficoll 70/400 was infused intravenously (i.v.) following an initial bolus dose. To raise... (More)
The purpose of the present study was to assess the role of diffusion and convection during filtration of Ficoll across the glomerular filter by comparing glomerular sieving coefficients ( h) to neutral fluorescein isothiocyanate ( FITC)-Ficoll 70/400 obtained at low ( hydropenic) vs raised ( normal) glomerular filtration rates (GFRs). The h for FITC-Ficoll was determined in anesthetized Wistar rats (304 +/- 18 g) following laparotomy and cannulation of the ureters, used for urine sampling. After surgery, GFR was 1.2 +/- 0.16 ml/ min (+/- s. e.), assessed using the plasma to urine clearance of FITC-inulin and Cr-51-ethylenediaminetetraacetic acid. FITC-Ficoll 70/400 was infused intravenously (i.v.) following an initial bolus dose. To raise GFR, to an average of similar to 2 ml/ min, 5 ml of serum together with glucagon ( 3 mu g/min) was given i.v. FITC- inulin and FITC- Ficoll were determined in plasma and urine using size-exclusion high-performance liquid chromatography. The h for Ficoll as a function of Stokes - Einstein radius was significantly reduced in the range of 13 - 43 angstrom when GFR was raised. The maximal h lowering effect, in relative terms, of raising GFR was obtained for a Ficoll a(e) of similar to 32 angstrom. For Ficoll(36 angstrom) (cf. albumin), h was reduced from 0.111 +/- 0.009 to 0.081 +/- 0.012 ( P<0.05; n = 7) for the GFR increment imposed. The reduction in h for Ficoll after raising GFR indicates the presence of a high diffusive component of glomerular Ficoll filtration in rats in vivo and contradicts the notion of a significant concentration polarization effect in the glomerular filter upon Ficoll molecules <50 angstrom in radius. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
reflection coefficient, macromolecules, filtration barrier, concentration polarization, albumin, capillary permeability
in
Kidney International
volume
69
issue
8
pages
1326 - 1332
publisher
Nature Publishing Group
external identifiers
  • wos:000237008600012
  • pmid:16395274
  • scopus:33646150223
ISSN
1523-1755
DOI
10.1038/sj.ki.5000027
language
English
LU publication?
yes
id
8b7019d8-b280-4290-8e09-ab30674cde31 (old id 150485)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16395274&dopt=Abstract
date added to LUP
2007-07-10 11:50:41
date last changed
2019-04-23 02:53:58
@article{8b7019d8-b280-4290-8e09-ab30674cde31,
  abstract     = {The purpose of the present study was to assess the role of diffusion and convection during filtration of Ficoll across the glomerular filter by comparing glomerular sieving coefficients ( h) to neutral fluorescein isothiocyanate ( FITC)-Ficoll 70/400 obtained at low ( hydropenic) vs raised ( normal) glomerular filtration rates (GFRs). The h for FITC-Ficoll was determined in anesthetized Wistar rats (304 +/- 18 g) following laparotomy and cannulation of the ureters, used for urine sampling. After surgery, GFR was 1.2 +/- 0.16 ml/ min (+/- s. e.), assessed using the plasma to urine clearance of FITC-inulin and Cr-51-ethylenediaminetetraacetic acid. FITC-Ficoll 70/400 was infused intravenously (i.v.) following an initial bolus dose. To raise GFR, to an average of similar to 2 ml/ min, 5 ml of serum together with glucagon ( 3 mu g/min) was given i.v. FITC- inulin and FITC- Ficoll were determined in plasma and urine using size-exclusion high-performance liquid chromatography. The h for Ficoll as a function of Stokes - Einstein radius was significantly reduced in the range of 13 - 43 angstrom when GFR was raised. The maximal h lowering effect, in relative terms, of raising GFR was obtained for a Ficoll a(e) of similar to 32 angstrom. For Ficoll(36 angstrom) (cf. albumin), h was reduced from 0.111 +/- 0.009 to 0.081 +/- 0.012 ( P&lt;0.05; n = 7) for the GFR increment imposed. The reduction in h for Ficoll after raising GFR indicates the presence of a high diffusive component of glomerular Ficoll filtration in rats in vivo and contradicts the notion of a significant concentration polarization effect in the glomerular filter upon Ficoll molecules &lt;50 angstrom in radius.},
  author       = {Rippe, Catarina and Asgeirsson, Daniel and Venturoli, Daniele and Rippe, Anna and Rippe, Bengt},
  issn         = {1523-1755},
  keyword      = {reflection coefficient,macromolecules,filtration barrier,concentration polarization,albumin,capillary permeability},
  language     = {eng},
  number       = {8},
  pages        = {1326--1332},
  publisher    = {Nature Publishing Group},
  series       = {Kidney International},
  title        = {Effects of glomerular filtration rate on Ficoll sieving coefficients (theta) in rats.},
  url          = {http://dx.doi.org/10.1038/sj.ki.5000027},
  volume       = {69},
  year         = {2006},
}