Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Specific effects of platelet derived growth factor (PDGF) on fetal rat and human dopaminergic neurons in vitro

Othberg, A LU ; Odin, P LU orcid ; Ballagi, A ; Ahgren, A ; Funa, K and Lindvall, O LU (1995) In Experimental Brain Research 105(1). p.22-111
Abstract

The neurotrophic effects of the BB isoform of platelet-derived growth factor (PDGF) on rat and human fetal mesencephalic dopaminergic neurons have been characterized in vitro. A dose-response analysis demonstrated maximal responses at 30 ng/ml of PDGF-BB. This concentration resulted in a marked increase in the survival and neurite outgrowth from rat and human tyrosine hydroxylase-(TH) positive, presumed dopaminergic neurons after 7 days in vitro. The effects of PDGF-BB on survival of TH-positive neurons were comparable to those of brain-derived neurotrophic factor (BDNF), whereas neurite outgrowth was more pronounced after addition of BDNF. The combination of BDNF and PDGF-BB yielded no additive effects. Double immunohistochemical... (More)

The neurotrophic effects of the BB isoform of platelet-derived growth factor (PDGF) on rat and human fetal mesencephalic dopaminergic neurons have been characterized in vitro. A dose-response analysis demonstrated maximal responses at 30 ng/ml of PDGF-BB. This concentration resulted in a marked increase in the survival and neurite outgrowth from rat and human tyrosine hydroxylase-(TH) positive, presumed dopaminergic neurons after 7 days in vitro. The effects of PDGF-BB on survival of TH-positive neurons were comparable to those of brain-derived neurotrophic factor (BDNF), whereas neurite outgrowth was more pronounced after addition of BDNF. The combination of BDNF and PDGF-BB yielded no additive effects. Double immunohistochemical staining of rat cultures demonstrated PDGF beta-receptors on about 90% of the TH-positive neurons. PDGF-BB treatment of rat mesencephalic cultures induced an upregulation of c-fos and TH mRNA with maximal levels after 0.5-2 h as assessed by quantitative PCR analysis. An increased number of Fos protein-positive cells was detected immunohistochemically after 4 h of PDGF-BB treatment. The present results provide further evidence for specific and direct effects of PDGF-BB on gene expression, survival and neurite outgrowth of mesencephalic dopaminergic neurons of rat and human origin.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Animals, Newborn, Cells, Cultured, Dopamine, Fetus, Humans, Immunohistochemistry, In Vitro Techniques, Neurons, Platelet-Derived Growth Factor, Polymerase Chain Reaction, Rats, Rats, Sprague-Dawley, Journal Article, Research Support, Non-U.S. Gov't
in
Experimental Brain Research
volume
105
issue
1
pages
12 pages
publisher
Springer
external identifiers
  • scopus:0029053764
  • pmid:7589308
ISSN
0014-4819
DOI
10.1007/BF00242187
language
English
LU publication?
yes
id
8bf0b2a2-668e-47c2-96c7-c99a9822e436
date added to LUP
2016-12-11 10:07:29
date last changed
2024-01-04 18:42:49
@article{8bf0b2a2-668e-47c2-96c7-c99a9822e436,
  abstract     = {{<p>The neurotrophic effects of the BB isoform of platelet-derived growth factor (PDGF) on rat and human fetal mesencephalic dopaminergic neurons have been characterized in vitro. A dose-response analysis demonstrated maximal responses at 30 ng/ml of PDGF-BB. This concentration resulted in a marked increase in the survival and neurite outgrowth from rat and human tyrosine hydroxylase-(TH) positive, presumed dopaminergic neurons after 7 days in vitro. The effects of PDGF-BB on survival of TH-positive neurons were comparable to those of brain-derived neurotrophic factor (BDNF), whereas neurite outgrowth was more pronounced after addition of BDNF. The combination of BDNF and PDGF-BB yielded no additive effects. Double immunohistochemical staining of rat cultures demonstrated PDGF beta-receptors on about 90% of the TH-positive neurons. PDGF-BB treatment of rat mesencephalic cultures induced an upregulation of c-fos and TH mRNA with maximal levels after 0.5-2 h as assessed by quantitative PCR analysis. An increased number of Fos protein-positive cells was detected immunohistochemically after 4 h of PDGF-BB treatment. The present results provide further evidence for specific and direct effects of PDGF-BB on gene expression, survival and neurite outgrowth of mesencephalic dopaminergic neurons of rat and human origin.</p>}},
  author       = {{Othberg, A and Odin, P and Ballagi, A and Ahgren, A and Funa, K and Lindvall, O}},
  issn         = {{0014-4819}},
  keywords     = {{Animals; Animals, Newborn; Cells, Cultured; Dopamine; Fetus; Humans; Immunohistochemistry; In Vitro Techniques; Neurons; Platelet-Derived Growth Factor; Polymerase Chain Reaction; Rats; Rats, Sprague-Dawley; Journal Article; Research Support, Non-U.S. Gov't}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{22--111}},
  publisher    = {{Springer}},
  series       = {{Experimental Brain Research}},
  title        = {{Specific effects of platelet derived growth factor (PDGF) on fetal rat and human dopaminergic neurons in vitro}},
  url          = {{http://dx.doi.org/10.1007/BF00242187}},
  doi          = {{10.1007/BF00242187}},
  volume       = {{105}},
  year         = {{1995}},
}