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Specific effects of platelet derived growth factor (PDGF) on fetal rat and human dopaminergic neurons in vitro

Othberg, A LU ; Odin, P LU ; Ballagi, A; Ahgren, A; Funa, K and Lindvall, O LU (1995) In Experimental Brain Research 105(1). p.22-111
Abstract

The neurotrophic effects of the BB isoform of platelet-derived growth factor (PDGF) on rat and human fetal mesencephalic dopaminergic neurons have been characterized in vitro. A dose-response analysis demonstrated maximal responses at 30 ng/ml of PDGF-BB. This concentration resulted in a marked increase in the survival and neurite outgrowth from rat and human tyrosine hydroxylase-(TH) positive, presumed dopaminergic neurons after 7 days in vitro. The effects of PDGF-BB on survival of TH-positive neurons were comparable to those of brain-derived neurotrophic factor (BDNF), whereas neurite outgrowth was more pronounced after addition of BDNF. The combination of BDNF and PDGF-BB yielded no additive effects. Double immunohistochemical... (More)

The neurotrophic effects of the BB isoform of platelet-derived growth factor (PDGF) on rat and human fetal mesencephalic dopaminergic neurons have been characterized in vitro. A dose-response analysis demonstrated maximal responses at 30 ng/ml of PDGF-BB. This concentration resulted in a marked increase in the survival and neurite outgrowth from rat and human tyrosine hydroxylase-(TH) positive, presumed dopaminergic neurons after 7 days in vitro. The effects of PDGF-BB on survival of TH-positive neurons were comparable to those of brain-derived neurotrophic factor (BDNF), whereas neurite outgrowth was more pronounced after addition of BDNF. The combination of BDNF and PDGF-BB yielded no additive effects. Double immunohistochemical staining of rat cultures demonstrated PDGF beta-receptors on about 90% of the TH-positive neurons. PDGF-BB treatment of rat mesencephalic cultures induced an upregulation of c-fos and TH mRNA with maximal levels after 0.5-2 h as assessed by quantitative PCR analysis. An increased number of Fos protein-positive cells was detected immunohistochemically after 4 h of PDGF-BB treatment. The present results provide further evidence for specific and direct effects of PDGF-BB on gene expression, survival and neurite outgrowth of mesencephalic dopaminergic neurons of rat and human origin.

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keywords
Animals, Animals, Newborn, Cells, Cultured, Dopamine, Fetus, Humans, Immunohistochemistry, In Vitro Techniques, Neurons, Platelet-Derived Growth Factor, Polymerase Chain Reaction, Rats, Rats, Sprague-Dawley, Journal Article, Research Support, Non-U.S. Gov't
in
Experimental Brain Research
volume
105
issue
1
pages
12 pages
publisher
Springer
external identifiers
  • scopus:0029053764
ISSN
0014-4819
DOI
10.1007/BF00242187
language
English
LU publication?
yes
id
8bf0b2a2-668e-47c2-96c7-c99a9822e436
date added to LUP
2016-12-11 10:07:29
date last changed
2017-04-30 17:51:34
@article{8bf0b2a2-668e-47c2-96c7-c99a9822e436,
  abstract     = {<p>The neurotrophic effects of the BB isoform of platelet-derived growth factor (PDGF) on rat and human fetal mesencephalic dopaminergic neurons have been characterized in vitro. A dose-response analysis demonstrated maximal responses at 30 ng/ml of PDGF-BB. This concentration resulted in a marked increase in the survival and neurite outgrowth from rat and human tyrosine hydroxylase-(TH) positive, presumed dopaminergic neurons after 7 days in vitro. The effects of PDGF-BB on survival of TH-positive neurons were comparable to those of brain-derived neurotrophic factor (BDNF), whereas neurite outgrowth was more pronounced after addition of BDNF. The combination of BDNF and PDGF-BB yielded no additive effects. Double immunohistochemical staining of rat cultures demonstrated PDGF beta-receptors on about 90% of the TH-positive neurons. PDGF-BB treatment of rat mesencephalic cultures induced an upregulation of c-fos and TH mRNA with maximal levels after 0.5-2 h as assessed by quantitative PCR analysis. An increased number of Fos protein-positive cells was detected immunohistochemically after 4 h of PDGF-BB treatment. The present results provide further evidence for specific and direct effects of PDGF-BB on gene expression, survival and neurite outgrowth of mesencephalic dopaminergic neurons of rat and human origin.</p>},
  author       = {Othberg, A and Odin, P and Ballagi, A and Ahgren, A and Funa, K and Lindvall, O},
  issn         = {0014-4819},
  keyword      = {Animals,Animals, Newborn,Cells, Cultured,Dopamine,Fetus,Humans,Immunohistochemistry,In Vitro Techniques,Neurons,Platelet-Derived Growth Factor,Polymerase Chain Reaction,Rats,Rats, Sprague-Dawley,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {1},
  pages        = {22--111},
  publisher    = {Springer},
  series       = {Experimental Brain Research},
  title        = {Specific effects of platelet derived growth factor (PDGF) on fetal rat and human dopaminergic neurons in vitro},
  url          = {http://dx.doi.org/10.1007/BF00242187},
  volume       = {105},
  year         = {1995},
}