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Human Chemokines as Antimicrobial Peptides with Direct Parasiticidal Effect on Leishmania mexicana In Vitro.

Karlsson Söbirk, Sara LU ; Mörgelin, Matthias LU ; Egesten, Arne LU ; Bates, Paul ; Shannon, Oonagh LU and Collin, Mattias LU (2013) In PLoS ONE 8(3).
Abstract
Chemokines and chemokine receptor-mediated effects are important mediators of the immunological response and cure in human leishmaniasis. However, in addition to their signalling properties for leukocytes, many chemokines have also been shown to act directly as antimicrobial peptides on bacteria and fungi. We screened ten human chemokines (CXCL2, CXCL6, CXCL8, CXCL9, CXCL10, CCL2, CCL3, CCL20, CCL27, CCL28) for antimicrobial effects on the promastigote form of the protozoan parasite Leishmania mexicana, and observed direct parasiticidal effects of several, CCL28 being the most potent. Damage to the plasma membrane integrity could be visualised by entrance of propidium iodide, as measured with flow cytometry, and by scanning electron... (More)
Chemokines and chemokine receptor-mediated effects are important mediators of the immunological response and cure in human leishmaniasis. However, in addition to their signalling properties for leukocytes, many chemokines have also been shown to act directly as antimicrobial peptides on bacteria and fungi. We screened ten human chemokines (CXCL2, CXCL6, CXCL8, CXCL9, CXCL10, CCL2, CCL3, CCL20, CCL27, CCL28) for antimicrobial effects on the promastigote form of the protozoan parasite Leishmania mexicana, and observed direct parasiticidal effects of several, CCL28 being the most potent. Damage to the plasma membrane integrity could be visualised by entrance of propidium iodide, as measured with flow cytometry, and by scanning electron microscopy, which showed morphological changes and aggregation of cells. The findings were in concordance with parasiticidal activity, measured by decreased mitochondrial activity in an MTT-assay. This is the first report of direct antimicrobial activity by chemokines on parasites. This component of immunity against Leishmania parasites identified here warrants further investigation that might lead to new insight in the mechanisms of human infection and/or new therapeutic approaches. (Less)
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publication status
published
subject
in
PLoS ONE
volume
8
issue
3
article number
e58129
publisher
Public Library of Science
external identifiers
  • wos:000316549400019
  • pmid:23533582
  • scopus:84875319509
  • pmid:23533582
ISSN
1932-6203
DOI
10.1371/journal.pone.0058129
language
English
LU publication?
yes
id
8c5d2316-1d49-4280-9433-5b3e1d035da9 (old id 3627631)
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http://www.ncbi.nlm.nih.gov/pubmed/23533582?dopt=Abstract
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2016-04-01 14:37:41
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2020-09-23 04:48:30
@article{8c5d2316-1d49-4280-9433-5b3e1d035da9,
  abstract     = {Chemokines and chemokine receptor-mediated effects are important mediators of the immunological response and cure in human leishmaniasis. However, in addition to their signalling properties for leukocytes, many chemokines have also been shown to act directly as antimicrobial peptides on bacteria and fungi. We screened ten human chemokines (CXCL2, CXCL6, CXCL8, CXCL9, CXCL10, CCL2, CCL3, CCL20, CCL27, CCL28) for antimicrobial effects on the promastigote form of the protozoan parasite Leishmania mexicana, and observed direct parasiticidal effects of several, CCL28 being the most potent. Damage to the plasma membrane integrity could be visualised by entrance of propidium iodide, as measured with flow cytometry, and by scanning electron microscopy, which showed morphological changes and aggregation of cells. The findings were in concordance with parasiticidal activity, measured by decreased mitochondrial activity in an MTT-assay. This is the first report of direct antimicrobial activity by chemokines on parasites. This component of immunity against Leishmania parasites identified here warrants further investigation that might lead to new insight in the mechanisms of human infection and/or new therapeutic approaches.},
  author       = {Karlsson Söbirk, Sara and Mörgelin, Matthias and Egesten, Arne and Bates, Paul and Shannon, Oonagh and Collin, Mattias},
  issn         = {1932-6203},
  language     = {eng},
  number       = {3},
  publisher    = {Public Library of Science},
  series       = {PLoS ONE},
  title        = {Human Chemokines as Antimicrobial Peptides with Direct Parasiticidal Effect on Leishmania mexicana In Vitro.},
  url          = {https://lup.lub.lu.se/search/ws/files/4073463/3735823.pdf},
  doi          = {10.1371/journal.pone.0058129},
  volume       = {8},
  year         = {2013},
}