Effect of pulsed electric field on the germination of barley seeds
Dymek, Katarzyna LU ; Dejmek, Petr LU
; Panarese, Valentina
; Vicente, Antonio A.
; Wadsö, Lars
LU
; Finnie, Christine
and Gomez, Federico
LU
(2012)
In LWT - Food Science and Technology
47(1).
p.161-166
- Abstract
- This study explores metabolic responses of germinating barley seeds upon the application of pulsed electric fields (PEE). Malting barley seeds were steeped in aerated water for 24 h and PEF-treated at varying voltages (0 (control), 110, 160, 240, 320, 400 and 480 V). The seeds were then allowed to finish germination in saturated air. It is shown that exposure of germinating barley to PEE affects radicle emergence without significantly affecting the seeds gross metabolic activity, as quantified by isothermal calorimetry. An exploration of protein 2-DE profiles of both the embryo and the starchy endosperm showed that, at the studied time scale, no significant changes were found in proteins present at concentrations higher than the detection... (More)
- This study explores metabolic responses of germinating barley seeds upon the application of pulsed electric fields (PEE). Malting barley seeds were steeped in aerated water for 24 h and PEF-treated at varying voltages (0 (control), 110, 160, 240, 320, 400 and 480 V). The seeds were then allowed to finish germination in saturated air. It is shown that exposure of germinating barley to PEE affects radicle emergence without significantly affecting the seeds gross metabolic activity, as quantified by isothermal calorimetry. An exploration of protein 2-DE profiles of both the embryo and the starchy endosperm showed that, at the studied time scale, no significant changes were found in proteins present at concentrations higher than the detection limit. However, western blotting demonstrated that a-amylase concentration decreases in the PEF-treated seeds. (C) 2011 Elsevier Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2494959
- author
- Dymek, Katarzyna
LU
; Dejmek, Petr
LU
; Panarese, Valentina
; Vicente, Antonio A.
; Wadsö, Lars
LU
; Finnie, Christine
and Gomez, Federico
LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Isothermal calorimetry, Radical emergence, alpha-Amylase
- in
- LWT - Food Science and Technology
- volume
- 47
- issue
- 1
- pages
- 161 - 166
- publisher
- Elsevier
- external identifiers
-
- wos:000301750100024
- scopus:84857110933
- ISSN
- 0023-6438
- DOI
- 10.1016/j.lwt.2011.12.019
- language
- English
- LU publication?
- yes
- id
- 8c75cdd7-75fb-4a36-88d9-877702efd25f (old id 2494959)
- date added to LUP
- 2016-04-01 13:18:24
- date last changed
- 2023-11-12 15:03:36
@article{8c75cdd7-75fb-4a36-88d9-877702efd25f,
abstract = {{This study explores metabolic responses of germinating barley seeds upon the application of pulsed electric fields (PEE). Malting barley seeds were steeped in aerated water for 24 h and PEF-treated at varying voltages (0 (control), 110, 160, 240, 320, 400 and 480 V). The seeds were then allowed to finish germination in saturated air. It is shown that exposure of germinating barley to PEE affects radicle emergence without significantly affecting the seeds gross metabolic activity, as quantified by isothermal calorimetry. An exploration of protein 2-DE profiles of both the embryo and the starchy endosperm showed that, at the studied time scale, no significant changes were found in proteins present at concentrations higher than the detection limit. However, western blotting demonstrated that a-amylase concentration decreases in the PEF-treated seeds. (C) 2011 Elsevier Ltd. All rights reserved.}},
author = {{Dymek, Katarzyna and Dejmek, Petr and Panarese, Valentina and Vicente, Antonio A. and Wadsö, Lars and Finnie, Christine and Gomez, Federico}},
issn = {{0023-6438}},
keywords = {{Isothermal calorimetry; Radical emergence; alpha-Amylase}},
language = {{eng}},
number = {{1}},
pages = {{161--166}},
publisher = {{Elsevier}},
series = {{LWT - Food Science and Technology}},
title = {{Effect of pulsed electric field on the germination of barley seeds}},
url = {{http://dx.doi.org/10.1016/j.lwt.2011.12.019}},
doi = {{10.1016/j.lwt.2011.12.019}},
volume = {{47}},
year = {{2012}},
}