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Alternative biomarkers for classification of latent tuberculosis infection status in pregnant women with borderline Quantiferon plus results

Tesfaye, Fregenet LU ; Sturegård, Erik LU ; Walles, John LU orcid ; Winqvist, Niclas LU ; Balcha, Taye Tolera LU ; Karlson, Sara LU orcid ; Mulleta, Daba ; Isberg, Per Erik LU ; Jansson, Marianne LU and Björkman, Per LU orcid (2020) In Tuberculosis 124.
Abstract

Borderline interferon-gamma (IFN-γ) results (near the cut-off level 0.35 IU/ml) occur in QuantiFERON (QFT) assays. We investigated the performance of alternative biomarkers for classification of latent tuberculosis infection (LTBI) status in pregnant women with borderline QFT IFN-γ responses. Pregnant women (n = 96) were identified from a cohort study in Ethiopia, based on QFT-Plus IFN-γ results (QFT-low: <0.20 IU/ml, n = 33; QFT-borderline: 0.20–0.70 IU/ml, n = 31; QFT-high: >0.70 IU/ml, n = 32), including 12 HIV-positive individuals in each group and with 20 HIV-negative non-pregnant women from the same cohort with QFT IFN-γ <0.20 IU/ml as controls. Concentrations of 8 markers (IL-1ra, IL-6, IL-8, IP-10, MCP-1, MCP-2,... (More)

Borderline interferon-gamma (IFN-γ) results (near the cut-off level 0.35 IU/ml) occur in QuantiFERON (QFT) assays. We investigated the performance of alternative biomarkers for classification of latent tuberculosis infection (LTBI) status in pregnant women with borderline QFT IFN-γ responses. Pregnant women (n = 96) were identified from a cohort study in Ethiopia, based on QFT-Plus IFN-γ results (QFT-low: <0.20 IU/ml, n = 33; QFT-borderline: 0.20–0.70 IU/ml, n = 31; QFT-high: >0.70 IU/ml, n = 32), including 12 HIV-positive individuals in each group and with 20 HIV-negative non-pregnant women from the same cohort with QFT IFN-γ <0.20 IU/ml as controls. Concentrations of 8 markers (IL-1ra, IL-6, IL-8, IP-10, MCP-1, MCP-2, osteopontin and resistin) were measured in whole blood QFT supernatants, stimulated separately with TB1 and TB2 antigens. K-nearest neighbor analysis (KNN) was used to classify participants with regard to likelihood of LTBI. Concentrations of MCP-2, IP-10 and IL-1ra were higher in QFT-borderline compared to QFT-low participants in both antigen stimulations (p < 0.001). KNN classification indicated high likelihood of LTBI in 13/31 (42%) women with QFT-borderline IFN-γ results. MCP-2, IP-10 and IL-1ra expressed in whole blood after TB antigen stimulation may be considered as alternative biomarkers for classification of LTBI status in pregnant women with borderline QFT IFN-γ results.

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author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biomarkers, Ethiopia, Gold plus, Latent tuberculosis, Pregnancy, QFT borderline, Quantiferon, TB
in
Tuberculosis
volume
124
article number
101984
publisher
Elsevier
external identifiers
  • pmid:32829076
  • scopus:85089491588
ISSN
1472-9792
DOI
10.1016/j.tube.2020.101984
language
English
LU publication?
yes
id
8c85ead3-5e16-41cf-aaaf-b7292d9dad16
date added to LUP
2020-08-27 12:04:10
date last changed
2025-01-23 19:33:16
@article{8c85ead3-5e16-41cf-aaaf-b7292d9dad16,
  abstract     = {{<p>Borderline interferon-gamma (IFN-γ) results (near the cut-off level 0.35 IU/ml) occur in QuantiFERON (QFT) assays. We investigated the performance of alternative biomarkers for classification of latent tuberculosis infection (LTBI) status in pregnant women with borderline QFT IFN-γ responses. Pregnant women (n = 96) were identified from a cohort study in Ethiopia, based on QFT-Plus IFN-γ results (QFT-low: &lt;0.20 IU/ml, n = 33; QFT-borderline: 0.20–0.70 IU/ml, n = 31; QFT-high: &gt;0.70 IU/ml, n = 32), including 12 HIV-positive individuals in each group and with 20 HIV-negative non-pregnant women from the same cohort with QFT IFN-γ &lt;0.20 IU/ml as controls. Concentrations of 8 markers (IL-1ra, IL-6, IL-8, IP-10, MCP-1, MCP-2, osteopontin and resistin) were measured in whole blood QFT supernatants, stimulated separately with TB1 and TB2 antigens. K-nearest neighbor analysis (KNN) was used to classify participants with regard to likelihood of LTBI. Concentrations of MCP-2, IP-10 and IL-1ra were higher in QFT-borderline compared to QFT-low participants in both antigen stimulations (p &lt; 0.001). KNN classification indicated high likelihood of LTBI in 13/31 (42%) women with QFT-borderline IFN-γ results. MCP-2, IP-10 and IL-1ra expressed in whole blood after TB antigen stimulation may be considered as alternative biomarkers for classification of LTBI status in pregnant women with borderline QFT IFN-γ results.</p>}},
  author       = {{Tesfaye, Fregenet and Sturegård, Erik and Walles, John and Winqvist, Niclas and Balcha, Taye Tolera and Karlson, Sara and Mulleta, Daba and Isberg, Per Erik and Jansson, Marianne and Björkman, Per}},
  issn         = {{1472-9792}},
  keywords     = {{Biomarkers; Ethiopia; Gold plus; Latent tuberculosis; Pregnancy; QFT borderline; Quantiferon; TB}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Tuberculosis}},
  title        = {{Alternative biomarkers for classification of latent tuberculosis infection status in pregnant women with borderline Quantiferon plus results}},
  url          = {{http://dx.doi.org/10.1016/j.tube.2020.101984}},
  doi          = {{10.1016/j.tube.2020.101984}},
  volume       = {{124}},
  year         = {{2020}},
}