Size-based isolation and detection of renal carcinoma cells from whole blood
(2022) In Molecular and clinical oncology 16(5).- Abstract
Renal cell carcinoma (RCC) is a tumour type with an indolent growth pattern and rather vague symptoms. The present study developed a platform for liquid biopsy of RCC based upon the isolation of circulating tumour cells (CTCs). Founded on the observation that RCC tumour cells are considerably larger than leucocytes, the present study employed a microfluidics-based system for isolation of RCC CTCs from whole blood. Using this system, it was revealed that 66% of spiked-in RCC tumour cells could be retrieved using this approach. Furthermore, it was demonstrated that these cells could be molecularly detected with digital PCR using RCC-specific genes down to one tumour cell, whilst avoiding detection in samples lacking tumour cells. Finally,... (More)
Renal cell carcinoma (RCC) is a tumour type with an indolent growth pattern and rather vague symptoms. The present study developed a platform for liquid biopsy of RCC based upon the isolation of circulating tumour cells (CTCs). Founded on the observation that RCC tumour cells are considerably larger than leucocytes, the present study employed a microfluidics-based system for isolation of RCC CTCs from whole blood. Using this system, it was revealed that 66% of spiked-in RCC tumour cells could be retrieved using this approach. Furthermore, it was demonstrated that these cells could be molecularly detected with digital PCR using RCC-specific genes down to one tumour cell, whilst avoiding detection in samples lacking tumour cells. Finally, subtype specific transcripts were identified to distinguish the different subtypes of RCC, which were then validated in patient tumours. The present study established a novel workflow for the isolation of RCC CTCs from whole blood, with the potential to detect these cells irrespective of subtype.
(Less)
- author
- organization
- publishing date
- 2022-03-21
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- circulating tumour cells, kidney cancer, liquid biopsy, molecular diagnostics
- in
- Molecular and clinical oncology
- volume
- 16
- issue
- 5
- article number
- 101
- pages
- 11 pages
- publisher
- Spandidos Publications
- external identifiers
-
- scopus:85129040042
- pmid:35463211
- ISSN
- 2049-9450
- DOI
- 10.3892/mco.2022.2534
- language
- English
- LU publication?
- yes
- additional info
- Copyright: © Alwis et al.
- id
- 8cb67fb0-8f27-42c2-8089-4cda7b35c246
- date added to LUP
- 2022-05-18 12:38:02
- date last changed
- 2024-10-18 02:32:23
@article{8cb67fb0-8f27-42c2-8089-4cda7b35c246, abstract = {{<p>Renal cell carcinoma (RCC) is a tumour type with an indolent growth pattern and rather vague symptoms. The present study developed a platform for liquid biopsy of RCC based upon the isolation of circulating tumour cells (CTCs). Founded on the observation that RCC tumour cells are considerably larger than leucocytes, the present study employed a microfluidics-based system for isolation of RCC CTCs from whole blood. Using this system, it was revealed that 66% of spiked-in RCC tumour cells could be retrieved using this approach. Furthermore, it was demonstrated that these cells could be molecularly detected with digital PCR using RCC-specific genes down to one tumour cell, whilst avoiding detection in samples lacking tumour cells. Finally, subtype specific transcripts were identified to distinguish the different subtypes of RCC, which were then validated in patient tumours. The present study established a novel workflow for the isolation of RCC CTCs from whole blood, with the potential to detect these cells irrespective of subtype.</p>}}, author = {{De Alwis, Roger and Hansson, Jennifer and Lindgren, David and Schoch, Sarah and Tejera, Alexander and Scholtz, Bianca and Elfving, Peter and Möller, Christina and Nilsson, Helén and Johansson, Martin and Axelson, Håkan}}, issn = {{2049-9450}}, keywords = {{circulating tumour cells; kidney cancer; liquid biopsy; molecular diagnostics}}, language = {{eng}}, month = {{03}}, number = {{5}}, publisher = {{Spandidos Publications}}, series = {{Molecular and clinical oncology}}, title = {{Size-based isolation and detection of renal carcinoma cells from whole blood}}, url = {{http://dx.doi.org/10.3892/mco.2022.2534}}, doi = {{10.3892/mco.2022.2534}}, volume = {{16}}, year = {{2022}}, }