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Size-based isolation and detection of renal carcinoma cells from whole blood

De Alwis, Roger LU ; Hansson, Jennifer LU ; Lindgren, David LU ; Schoch, Sarah LU ; Tejera, Alexander LU orcid ; Scholtz, Bianca LU ; Elfving, Peter LU ; Möller, Christina LU ; Nilsson, Helén LU and Johansson, Martin LU , et al. (2022) In Molecular and clinical oncology 16(5).
Abstract

Renal cell carcinoma (RCC) is a tumour type with an indolent growth pattern and rather vague symptoms. The present study developed a platform for liquid biopsy of RCC based upon the isolation of circulating tumour cells (CTCs). Founded on the observation that RCC tumour cells are considerably larger than leucocytes, the present study employed a microfluidics-based system for isolation of RCC CTCs from whole blood. Using this system, it was revealed that 66% of spiked-in RCC tumour cells could be retrieved using this approach. Furthermore, it was demonstrated that these cells could be molecularly detected with digital PCR using RCC-specific genes down to one tumour cell, whilst avoiding detection in samples lacking tumour cells. Finally,... (More)

Renal cell carcinoma (RCC) is a tumour type with an indolent growth pattern and rather vague symptoms. The present study developed a platform for liquid biopsy of RCC based upon the isolation of circulating tumour cells (CTCs). Founded on the observation that RCC tumour cells are considerably larger than leucocytes, the present study employed a microfluidics-based system for isolation of RCC CTCs from whole blood. Using this system, it was revealed that 66% of spiked-in RCC tumour cells could be retrieved using this approach. Furthermore, it was demonstrated that these cells could be molecularly detected with digital PCR using RCC-specific genes down to one tumour cell, whilst avoiding detection in samples lacking tumour cells. Finally, subtype specific transcripts were identified to distinguish the different subtypes of RCC, which were then validated in patient tumours. The present study established a novel workflow for the isolation of RCC CTCs from whole blood, with the potential to detect these cells irrespective of subtype.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
circulating tumour cells, kidney cancer, liquid biopsy, molecular diagnostics
in
Molecular and clinical oncology
volume
16
issue
5
article number
101
pages
11 pages
publisher
Spandidos Publications
external identifiers
  • scopus:85129040042
  • pmid:35463211
ISSN
2049-9450
DOI
10.3892/mco.2022.2534
language
English
LU publication?
yes
additional info
Copyright: © Alwis et al.
id
8cb67fb0-8f27-42c2-8089-4cda7b35c246
date added to LUP
2022-05-18 12:38:02
date last changed
2024-06-13 12:46:34
@article{8cb67fb0-8f27-42c2-8089-4cda7b35c246,
  abstract     = {{<p>Renal cell carcinoma (RCC) is a tumour type with an indolent growth pattern and rather vague symptoms. The present study developed a platform for liquid biopsy of RCC based upon the isolation of circulating tumour cells (CTCs). Founded on the observation that RCC tumour cells are considerably larger than leucocytes, the present study employed a microfluidics-based system for isolation of RCC CTCs from whole blood. Using this system, it was revealed that 66% of spiked-in RCC tumour cells could be retrieved using this approach. Furthermore, it was demonstrated that these cells could be molecularly detected with digital PCR using RCC-specific genes down to one tumour cell, whilst avoiding detection in samples lacking tumour cells. Finally, subtype specific transcripts were identified to distinguish the different subtypes of RCC, which were then validated in patient tumours. The present study established a novel workflow for the isolation of RCC CTCs from whole blood, with the potential to detect these cells irrespective of subtype.</p>}},
  author       = {{De Alwis, Roger and Hansson, Jennifer and Lindgren, David and Schoch, Sarah and Tejera, Alexander and Scholtz, Bianca and Elfving, Peter and Möller, Christina and Nilsson, Helén and Johansson, Martin and Axelson, Håkan}},
  issn         = {{2049-9450}},
  keywords     = {{circulating tumour cells; kidney cancer; liquid biopsy; molecular diagnostics}},
  language     = {{eng}},
  month        = {{03}},
  number       = {{5}},
  publisher    = {{Spandidos Publications}},
  series       = {{Molecular and clinical oncology}},
  title        = {{Size-based isolation and detection of renal carcinoma cells from whole blood}},
  url          = {{http://dx.doi.org/10.3892/mco.2022.2534}},
  doi          = {{10.3892/mco.2022.2534}},
  volume       = {{16}},
  year         = {{2022}},
}