Asymmetrical flow field-flow fractionation enables the characterization of molecular and supramolecular properties of cereal beta-glucan dispersions
(2012) In Carbohydrate Polymers 87(1). p.518-523- Abstract
- In this paper we study the properties of molecular and supra molecular species in cereal beta-glucan solutions/dispersions by the utilization of asymmetrical flow field-flow fractionation coupled to multi-angle light scattering and refractive index (AsFlFFF-MALS-RI) detectors. The samples were purified barley and oat beta-glucans which were dissolved in aqueous solution using either mild conditions or more harsh treatments with alkali. Dissolution in 0.5 M NaOH was not sufficient to eliminate aggregated structures in barley beta-glucan. The results in this paper show how distinction can possibly be made between molecular and supra molecular species using scaling approaches and conformational parameters obtained from AsFlFFF-MALS-RI over... (More)
- In this paper we study the properties of molecular and supra molecular species in cereal beta-glucan solutions/dispersions by the utilization of asymmetrical flow field-flow fractionation coupled to multi-angle light scattering and refractive index (AsFlFFF-MALS-RI) detectors. The samples were purified barley and oat beta-glucans which were dissolved in aqueous solution using either mild conditions or more harsh treatments with alkali. Dissolution in 0.5 M NaOH was not sufficient to eliminate aggregated structures in barley beta-glucan. The results in this paper show how distinction can possibly be made between molecular and supra molecular species using scaling approaches and conformational parameters obtained from AsFlFFF-MALS-RI over the entire size distribution. Small species in the barley beta-glucan samples display properties ranging from elongated conformation to random coil conformation. Aggregates have
low apparent densities and a swollen micro gel structure. Oat beta-glucan displays no properties that can be attributed to a molecularly dissolved beta-glucan showing that dissolution was incomplete. The aggregate properties analyzed were similar between oat and barley beta-glucan. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2277730
- author
- Håkansson, Andreas LU ; Ulmius, Matilda LU and Nilsson, Lars LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Beta-glucan Asymmetrical flow field-flow fractionation AF4 Oat Barley
- in
- Carbohydrate Polymers
- volume
- 87
- issue
- 1
- pages
- 518 - 523
- publisher
- Elsevier
- external identifiers
-
- wos:000297389000068
- scopus:80054737009
- ISSN
- 0144-8617
- DOI
- 10.1016/j.carbpol.2011.08.014
- language
- English
- LU publication?
- yes
- id
- 8cbfbea0-196f-4f71-8069-16afcbc260ac (old id 2277730)
- date added to LUP
- 2016-04-01 10:09:24
- date last changed
- 2023-11-09 13:04:03
@article{8cbfbea0-196f-4f71-8069-16afcbc260ac,
abstract = {{In this paper we study the properties of molecular and supra molecular species in cereal beta-glucan solutions/dispersions by the utilization of asymmetrical flow field-flow fractionation coupled to multi-angle light scattering and refractive index (AsFlFFF-MALS-RI) detectors. The samples were purified barley and oat beta-glucans which were dissolved in aqueous solution using either mild conditions or more harsh treatments with alkali. Dissolution in 0.5 M NaOH was not sufficient to eliminate aggregated structures in barley beta-glucan. The results in this paper show how distinction can possibly be made between molecular and supra molecular species using scaling approaches and conformational parameters obtained from AsFlFFF-MALS-RI over the entire size distribution. Small species in the barley beta-glucan samples display properties ranging from elongated conformation to random coil conformation. Aggregates have<br/><br>
low apparent densities and a swollen micro gel structure. Oat beta-glucan displays no properties that can be attributed to a molecularly dissolved beta-glucan showing that dissolution was incomplete. The aggregate properties analyzed were similar between oat and barley beta-glucan.}},
author = {{Håkansson, Andreas and Ulmius, Matilda and Nilsson, Lars}},
issn = {{0144-8617}},
keywords = {{Beta-glucan Asymmetrical flow field-flow fractionation AF4 Oat Barley}},
language = {{eng}},
number = {{1}},
pages = {{518--523}},
publisher = {{Elsevier}},
series = {{Carbohydrate Polymers}},
title = {{Asymmetrical flow field-flow fractionation enables the characterization of molecular and supramolecular properties of cereal beta-glucan dispersions}},
url = {{http://dx.doi.org/10.1016/j.carbpol.2011.08.014}},
doi = {{10.1016/j.carbpol.2011.08.014}},
volume = {{87}},
year = {{2012}},
}