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Uncovering the cytotoxic effects of air pollution with multi-modal imaging of in vitro respiratory models

Al-Rekabi, Zeinab ; Dondi, Camilla ; Faruqui, Nilofar ; Siddiqui, Nazia S. ; Elowsson, Linda LU ; Rissler, Jenny LU ; Kåredal, Monica LU orcid ; Mudway, Ian ; Larsson-Callerfelt, Anna Karin LU orcid and Shaw, Michael (2023) In Royal Society Open Science 10(4).
Abstract

Annually, an estimated seven million deaths are linked to exposure to airborne pollutants. Despite extensive epidemiological evidence supporting clear associations between poor air quality and a range of short- and long-term health effects, there are considerable gaps in our understanding of the specific mechanisms by which pollutant exposure induces adverse biological responses at the cellular and tissue levels. The development of more complex, predictive, in vitro respiratory models, including two- and three-dimensional cell cultures, spheroids, organoids and tissue cultures, along with more realistic aerosol exposure systems, offers new opportunities to investigate the cytotoxic effects of airborne particulates under controlled... (More)

Annually, an estimated seven million deaths are linked to exposure to airborne pollutants. Despite extensive epidemiological evidence supporting clear associations between poor air quality and a range of short- and long-term health effects, there are considerable gaps in our understanding of the specific mechanisms by which pollutant exposure induces adverse biological responses at the cellular and tissue levels. The development of more complex, predictive, in vitro respiratory models, including two- and three-dimensional cell cultures, spheroids, organoids and tissue cultures, along with more realistic aerosol exposure systems, offers new opportunities to investigate the cytotoxic effects of airborne particulates under controlled laboratory conditions. Parallel advances in high-resolution microscopy have resulted in a range of in vitro imaging tools capable of visualizing and analysing biological systems across unprecedented scales of length, time and complexity. This article considers state-of-the-art in vitro respiratory models and aerosol exposure systems and how they can be interrogated using high-resolution microscopy techniques to investigate cell-pollutant interactions, from the uptake and trafficking of particles to structural and functional modification of subcellular organelles and cells. These data can provide a mechanistic basis from which to advance our understanding of the health effects of airborne particulate pollution and develop improved mitigation measures.

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author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bioimaging, cell, microscopy, respiratory toxicology, tissue culture models
in
Royal Society Open Science
volume
10
issue
4
article number
221426
pages
25 pages
publisher
Royal Society Publishing
external identifiers
  • pmid:37063998
  • scopus:85157993342
ISSN
2054-5703
DOI
10.1098/rsos.221426
language
English
LU publication?
yes
id
8ed54c8b-dd2d-42a1-ae11-611c4791772b
date added to LUP
2023-08-11 13:24:11
date last changed
2024-04-20 00:25:23
@article{8ed54c8b-dd2d-42a1-ae11-611c4791772b,
  abstract     = {{<p>Annually, an estimated seven million deaths are linked to exposure to airborne pollutants. Despite extensive epidemiological evidence supporting clear associations between poor air quality and a range of short- and long-term health effects, there are considerable gaps in our understanding of the specific mechanisms by which pollutant exposure induces adverse biological responses at the cellular and tissue levels. The development of more complex, predictive, in vitro respiratory models, including two- and three-dimensional cell cultures, spheroids, organoids and tissue cultures, along with more realistic aerosol exposure systems, offers new opportunities to investigate the cytotoxic effects of airborne particulates under controlled laboratory conditions. Parallel advances in high-resolution microscopy have resulted in a range of in vitro imaging tools capable of visualizing and analysing biological systems across unprecedented scales of length, time and complexity. This article considers state-of-the-art in vitro respiratory models and aerosol exposure systems and how they can be interrogated using high-resolution microscopy techniques to investigate cell-pollutant interactions, from the uptake and trafficking of particles to structural and functional modification of subcellular organelles and cells. These data can provide a mechanistic basis from which to advance our understanding of the health effects of airborne particulate pollution and develop improved mitigation measures.</p>}},
  author       = {{Al-Rekabi, Zeinab and Dondi, Camilla and Faruqui, Nilofar and Siddiqui, Nazia S. and Elowsson, Linda and Rissler, Jenny and Kåredal, Monica and Mudway, Ian and Larsson-Callerfelt, Anna Karin and Shaw, Michael}},
  issn         = {{2054-5703}},
  keywords     = {{bioimaging; cell; microscopy; respiratory toxicology; tissue culture models}},
  language     = {{eng}},
  month        = {{04}},
  number       = {{4}},
  publisher    = {{Royal Society Publishing}},
  series       = {{Royal Society Open Science}},
  title        = {{Uncovering the cytotoxic effects of air pollution with multi-modal imaging of in vitro respiratory models}},
  url          = {{http://dx.doi.org/10.1098/rsos.221426}},
  doi          = {{10.1098/rsos.221426}},
  volume       = {{10}},
  year         = {{2023}},
}