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Identification of novel proteolytic forms of osteocalcin in human urine

Ivaska, KK; Hellman, J; Likojarvi, J; Kakonen, SM; Gerdhem, Paul LU ; Åkesson, Kristina LU ; Obrant, Karl LU ; Pettersson, K and Vaananen, HK (2003) In Biochemical and Biophysical Research Communications 306(4). p.973-980
Abstract
In this study, we report the isolation and characterization of osteocalcin in human urine using mass spectrometry and N-terminal sequencing. Multiple proteolytic forms of osteocalcin were found, which consisted of 16-27 residues from the middle region of the molecule. Several fragments had residue Gly7 at the N-terminus and the most predominant was fragment 7-31. Additional fragments starting from residue Asp14 were detected in the samples of children and young adults. Immunochemical detection of urine osteocalcin fragments had a statistically significant negative correlation to bone mineral density in evaluation of urine samples from 75-year-old women. Thus, the measurement of osteocalcin fragments in urine may have potential applications... (More)
In this study, we report the isolation and characterization of osteocalcin in human urine using mass spectrometry and N-terminal sequencing. Multiple proteolytic forms of osteocalcin were found, which consisted of 16-27 residues from the middle region of the molecule. Several fragments had residue Gly7 at the N-terminus and the most predominant was fragment 7-31. Additional fragments starting from residue Asp14 were detected in the samples of children and young adults. Immunochemical detection of urine osteocalcin fragments had a statistically significant negative correlation to bone mineral density in evaluation of urine samples from 75-year-old women. Thus, the measurement of osteocalcin fragments in urine may have potential applications in diagnostics related to disorders of bone metabolism. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochemical and Biophysical Research Communications
volume
306
issue
4
pages
973 - 980
publisher
Elsevier
external identifiers
  • pmid:12821138
  • wos:000183992400027
  • scopus:0038095337
ISSN
1090-2104
DOI
10.1016/S0006-291X(03)01093-3
language
English
LU publication?
yes
id
a7ecd1ca-6f00-4ad9-aa11-945862fddbfd (old id 900196)
date added to LUP
2008-01-14 11:47:03
date last changed
2018-05-29 09:43:48
@article{a7ecd1ca-6f00-4ad9-aa11-945862fddbfd,
  abstract     = {In this study, we report the isolation and characterization of osteocalcin in human urine using mass spectrometry and N-terminal sequencing. Multiple proteolytic forms of osteocalcin were found, which consisted of 16-27 residues from the middle region of the molecule. Several fragments had residue Gly7 at the N-terminus and the most predominant was fragment 7-31. Additional fragments starting from residue Asp14 were detected in the samples of children and young adults. Immunochemical detection of urine osteocalcin fragments had a statistically significant negative correlation to bone mineral density in evaluation of urine samples from 75-year-old women. Thus, the measurement of osteocalcin fragments in urine may have potential applications in diagnostics related to disorders of bone metabolism.},
  author       = {Ivaska, KK and Hellman, J and Likojarvi, J and Kakonen, SM and Gerdhem, Paul and Åkesson, Kristina and Obrant, Karl and Pettersson, K and Vaananen, HK},
  issn         = {1090-2104},
  language     = {eng},
  number       = {4},
  pages        = {973--980},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Identification of novel proteolytic forms of osteocalcin in human urine},
  url          = {http://dx.doi.org/10.1016/S0006-291X(03)01093-3},
  volume       = {306},
  year         = {2003},
}