Dekkera/Brettanomyces yeasts for ethanol production from renewable sources under oxygen-limited and low-pH conditions
(2011) In Journal of Industrial Microbiology & Biotechnology 38(8). p.1079-1088- Abstract
- Industrial fermentation of lignocellulosic hydrolysates to ethanol requires microorganisms able to utilise a broad range of carbon sources and generate ethanol at high yield and productivity. D. bruxellensis has recently been reported to contaminate commercial ethanol processes, where it competes with Saccharomyces cerevisiae [4, 26]. In this work Brettanomyces/Dekkera yeasts were studied to explore their potential to produce ethanol from renewable sources under conditions suitable for industrial processes, such as oxygen-limited and low-pH conditions. Over 50 strains were analysed for their ability to utilise a variety of carbon sources, and some strains grew on cellobiose and pentoses. Two strains of D. bruxellensis were able to produce... (More)
- Industrial fermentation of lignocellulosic hydrolysates to ethanol requires microorganisms able to utilise a broad range of carbon sources and generate ethanol at high yield and productivity. D. bruxellensis has recently been reported to contaminate commercial ethanol processes, where it competes with Saccharomyces cerevisiae [4, 26]. In this work Brettanomyces/Dekkera yeasts were studied to explore their potential to produce ethanol from renewable sources under conditions suitable for industrial processes, such as oxygen-limited and low-pH conditions. Over 50 strains were analysed for their ability to utilise a variety of carbon sources, and some strains grew on cellobiose and pentoses. Two strains of D. bruxellensis were able to produce ethanol at high yield (0.44 g g(-1) glucose), comparable to those reported for S. cerevisiae. B. naardenensis was shown to be able to produce ethanol from xylose. To obtain ethanol from synthetic lignocellulosic hydrolysates we developed a two-step fermentation strategy: the first step under aerobic conditions for fast production of biomass from mixtures of hexoses and pentoses, followed by a second step under oxygen limitation to promote ethanol production. Under these conditions we obtained biomass and ethanol production on synthetic lignocellulosic hydrolysates, with ethanol yields ranging from 0.2 to 0.3 g g(-1) sugar. Hexoses, xylose and arabinose were consumed at the end of the process, resulting in 13 g l(-1) of ethanol, even in the presence of furfural. Our studies showed that Brettanomyces/Dekkera yeasts have clear potential for further development for industrial processes aimed at production of ethanol from renewable sources. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2091571
- author
- Galafassi, Silvia ; Merico, Annamaria ; Pizza, Francesca ; Hellborg, Linda LU ; Molinari, Francesco ; Piskur, Jure LU and Compagno, Concetta
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Ethanol, Dekkera, Brettanomyces, Pentoses, Renewable sources
- in
- Journal of Industrial Microbiology & Biotechnology
- volume
- 38
- issue
- 8
- pages
- 1079 - 1088
- publisher
- Springer
- external identifiers
-
- wos:000293002000021
- scopus:80051701328
- pmid:20936422
- ISSN
- 1367-5435
- DOI
- 10.1007/s10295-010-0885-4
- language
- English
- LU publication?
- yes
- id
- 91217e71-8182-4df3-9dde-77f84d48624f (old id 2091571)
- date added to LUP
- 2016-04-01 13:03:45
- date last changed
- 2022-04-21 19:28:57
@article{91217e71-8182-4df3-9dde-77f84d48624f,
abstract = {{Industrial fermentation of lignocellulosic hydrolysates to ethanol requires microorganisms able to utilise a broad range of carbon sources and generate ethanol at high yield and productivity. D. bruxellensis has recently been reported to contaminate commercial ethanol processes, where it competes with Saccharomyces cerevisiae [4, 26]. In this work Brettanomyces/Dekkera yeasts were studied to explore their potential to produce ethanol from renewable sources under conditions suitable for industrial processes, such as oxygen-limited and low-pH conditions. Over 50 strains were analysed for their ability to utilise a variety of carbon sources, and some strains grew on cellobiose and pentoses. Two strains of D. bruxellensis were able to produce ethanol at high yield (0.44 g g(-1) glucose), comparable to those reported for S. cerevisiae. B. naardenensis was shown to be able to produce ethanol from xylose. To obtain ethanol from synthetic lignocellulosic hydrolysates we developed a two-step fermentation strategy: the first step under aerobic conditions for fast production of biomass from mixtures of hexoses and pentoses, followed by a second step under oxygen limitation to promote ethanol production. Under these conditions we obtained biomass and ethanol production on synthetic lignocellulosic hydrolysates, with ethanol yields ranging from 0.2 to 0.3 g g(-1) sugar. Hexoses, xylose and arabinose were consumed at the end of the process, resulting in 13 g l(-1) of ethanol, even in the presence of furfural. Our studies showed that Brettanomyces/Dekkera yeasts have clear potential for further development for industrial processes aimed at production of ethanol from renewable sources.}},
author = {{Galafassi, Silvia and Merico, Annamaria and Pizza, Francesca and Hellborg, Linda and Molinari, Francesco and Piskur, Jure and Compagno, Concetta}},
issn = {{1367-5435}},
keywords = {{Ethanol; Dekkera; Brettanomyces; Pentoses; Renewable sources}},
language = {{eng}},
number = {{8}},
pages = {{1079--1088}},
publisher = {{Springer}},
series = {{Journal of Industrial Microbiology & Biotechnology}},
title = {{Dekkera/Brettanomyces yeasts for ethanol production from renewable sources under oxygen-limited and low-pH conditions}},
url = {{http://dx.doi.org/10.1007/s10295-010-0885-4}},
doi = {{10.1007/s10295-010-0885-4}},
volume = {{38}},
year = {{2011}},
}