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Diagnostic potential of plasma microRNA signatures in patients with deep-vein thrombosis

Wang, Xiao LU ; Sundquist, Kristina LU ; Elf, Johan L. LU ; Strandberg, Karin LU ; Svensson, Peter J. LU ; Hedelius, Anna LU ; Palmér, Karolina LU ; Memon, Ashfaque A. LU ; Sundquist, Jan LU and Zöller, Bengt LU (2016) In Thrombosis and Haemostasis 116(2). p.328-336
Abstract

For excluding deep-vein thrombosis (DVT), a negative D-dimer and low clinical probability are used to rule out DVT. Circulating microR-NAs (miRNAs) are stably present in the plasma, serum and other body fluids. Their diagnostic function has been investigated in many diseases but not in DVT. The aims of present study were to assess the diagnostic ability of plasma miRNAs in DVT and to examine their correlation with known markers of hypercoagulability, such as D-dimer and APC-PCI complex. Plasma samples were obtained from 238 patients (aged 16-95 years) with suspected DVT included in a prospective multicentre management study (SCORE). We first performed miRNA screening of plasma samples from three plasma pools containing plasma from 12... (More)

For excluding deep-vein thrombosis (DVT), a negative D-dimer and low clinical probability are used to rule out DVT. Circulating microR-NAs (miRNAs) are stably present in the plasma, serum and other body fluids. Their diagnostic function has been investigated in many diseases but not in DVT. The aims of present study were to assess the diagnostic ability of plasma miRNAs in DVT and to examine their correlation with known markers of hypercoagulability, such as D-dimer and APC-PCI complex. Plasma samples were obtained from 238 patients (aged 16-95 years) with suspected DVT included in a prospective multicentre management study (SCORE). We first performed miRNA screening of plasma samples from three plasma pools containing plasma from 12 patients with DVT and three plasma pools containing plasma from 12 patients without DVT using a microRNA Ready-to-use PCR Panel comprising 742 miRNA primer sets. Thirteen miRNAs that differentially expressed were further investigated by quantitative real-time (qRT)-PCR in the entire cohort. The plasma level of miR-424-5p (p=0.01) were significantly higher, whereas the levels of miR-136-5p (p=0.03) were significantly lower in DVT patients compared to patients without DVT. Receiver-operating characteristic curve analysis showed the area under the curve (AUC) values of 0.63 for miR-424-5p and 0.60 for miR-136-5p. The plasma level of miR-424-5p was associated with both D-dimer and APC-PCI complex levels (p<0.0001 and p=0.001, respectively). In conclusions, these findings indicate that certain miRNAs are associated with DVT and markers of hypercoagulability, though their diagnostic abilities are probably too low.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
APC-PCI complex, D-dimer, Deep venous thrombosis, Diagnosis, MicroRNA
in
Thrombosis and Haemostasis
volume
116
issue
2
pages
9 pages
publisher
F K Schattauer Verlag Gmbh
external identifiers
  • scopus:84982688432
  • wos:000381592600013
ISSN
0340-6245
DOI
10.1160/TH16-01-0071
language
English
LU publication?
yes
id
92849b67-41df-4824-b06f-98b241e039eb
date added to LUP
2016-09-20 14:41:24
date last changed
2017-06-11 05:08:29
@article{92849b67-41df-4824-b06f-98b241e039eb,
  abstract     = {<p>For excluding deep-vein thrombosis (DVT), a negative D-dimer and low clinical probability are used to rule out DVT. Circulating microR-NAs (miRNAs) are stably present in the plasma, serum and other body fluids. Their diagnostic function has been investigated in many diseases but not in DVT. The aims of present study were to assess the diagnostic ability of plasma miRNAs in DVT and to examine their correlation with known markers of hypercoagulability, such as D-dimer and APC-PCI complex. Plasma samples were obtained from 238 patients (aged 16-95 years) with suspected DVT included in a prospective multicentre management study (SCORE). We first performed miRNA screening of plasma samples from three plasma pools containing plasma from 12 patients with DVT and three plasma pools containing plasma from 12 patients without DVT using a microRNA Ready-to-use PCR Panel comprising 742 miRNA primer sets. Thirteen miRNAs that differentially expressed were further investigated by quantitative real-time (qRT)-PCR in the entire cohort. The plasma level of miR-424-5p (p=0.01) were significantly higher, whereas the levels of miR-136-5p (p=0.03) were significantly lower in DVT patients compared to patients without DVT. Receiver-operating characteristic curve analysis showed the area under the curve (AUC) values of 0.63 for miR-424-5p and 0.60 for miR-136-5p. The plasma level of miR-424-5p was associated with both D-dimer and APC-PCI complex levels (p&lt;0.0001 and p=0.001, respectively). In conclusions, these findings indicate that certain miRNAs are associated with DVT and markers of hypercoagulability, though their diagnostic abilities are probably too low.</p>},
  author       = {Wang, Xiao and Sundquist, Kristina and Elf, Johan L. and Strandberg, Karin and Svensson, Peter J. and Hedelius, Anna and Palmér, Karolina and Memon, Ashfaque A. and Sundquist, Jan and Zöller, Bengt},
  issn         = {0340-6245},
  keyword      = {APC-PCI complex,D-dimer,Deep venous thrombosis,Diagnosis,MicroRNA},
  language     = {eng},
  month        = {08},
  number       = {2},
  pages        = {328--336},
  publisher    = {F K Schattauer Verlag Gmbh},
  series       = {Thrombosis and Haemostasis},
  title        = {Diagnostic potential of plasma microRNA signatures in patients with deep-vein thrombosis},
  url          = {http://dx.doi.org/10.1160/TH16-01-0071},
  volume       = {116},
  year         = {2016},
}