Aquaglyceroporins and orthodox aquaporins in human adipocytes
(2022) In Biochimica et Biophysica Acta - Biomembranes 1864(1).- Abstract
Aquaporins play a crucial role in water homeostasis in the human body, and recently the physiological importance of aquaporins as glycerol channels have been demonstrated. The aquaglyceroporins (AQP3, AQP7, AQP9 and AQP10) represent key glycerol channels, enabling glycerol flux across the membranes of cells. Adipocytes are the major source of glycerol and during lipolysis, glycerol is released to be metabolized by other tissues through a well-orchestrated process. Here we show that both AQP3 and AQP7 bind to the lipid droplet protein perilipin 1 (PLIN1), suggesting that PLIN1 is involved in the coordination of the subcellular translocation of aquaglyceroporins in human adipocytes. Moreover, in addition to aquaglyceroporins, we... (More)
Aquaporins play a crucial role in water homeostasis in the human body, and recently the physiological importance of aquaporins as glycerol channels have been demonstrated. The aquaglyceroporins (AQP3, AQP7, AQP9 and AQP10) represent key glycerol channels, enabling glycerol flux across the membranes of cells. Adipocytes are the major source of glycerol and during lipolysis, glycerol is released to be metabolized by other tissues through a well-orchestrated process. Here we show that both AQP3 and AQP7 bind to the lipid droplet protein perilipin 1 (PLIN1), suggesting that PLIN1 is involved in the coordination of the subcellular translocation of aquaglyceroporins in human adipocytes. Moreover, in addition to aquaglyceroporins, we discovered by transcriptome sequencing that AQP1 is expressed in human primary adipocytes. AQP1 is mainly a water channel and thus is thought to be involved in the response to hyper-osmotic stress by efflux of water during hyperglycemia. Thus, this data suggests a contribution of both orthodox aquaporin and aquaglyceroporin in human adipocytes to maintain the homeostasis of glycerol and water during fasting and feeding.
(Less)
- author
- Huang, Peng
LU
; Hansen, Jesper S.
; Saba, Karim H.
LU
; Bergman, Anna LU ; Negoita, Florentina LU ; Gourdon, Pontus LU ; Hagström-Andersson, Anna LU
and Lindkvist-Petersson, Karin LU
- organization
-
- EXODIAB: Excellence of Diabetes Research in Sweden
- Medical Structural Biology (research group)
- Genetic chaos in aggressive cancer (research group)
- Division of Clinical Genetics
- The pathogenetic mechanisms behind MLL-rearranged acute leukemia in infancy (research group)
- Protein Phosphorylation (research group)
- Membrane Protein Structural Biology (research group)
- publishing date
- 2022-02-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- AQP, Aquaglyceroporins, Human adipocytes, Orthodox aquaporins, Perilipin 1
- in
- Biochimica et Biophysica Acta - Biomembranes
- volume
- 1864
- issue
- 1
- article number
- 183795
- publisher
- Elsevier
- external identifiers
-
- scopus:85116860294
- pmid:34627746
- ISSN
- 0005-2736
- DOI
- 10.1016/j.bbamem.2021.183795
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2021 The Authors
- id
- 928de913-b3be-4801-b4e2-985f4cda91b8
- date added to LUP
- 2021-11-01 13:37:15
- date last changed
- 2025-01-26 18:38:52
@article{928de913-b3be-4801-b4e2-985f4cda91b8, abstract = {{<p>Aquaporins play a crucial role in water homeostasis in the human body, and recently the physiological importance of aquaporins as glycerol channels have been demonstrated. The aquaglyceroporins (AQP3, AQP7, AQP9 and AQP10) represent key glycerol channels, enabling glycerol flux across the membranes of cells. Adipocytes are the major source of glycerol and during lipolysis, glycerol is released to be metabolized by other tissues through a well-orchestrated process. Here we show that both AQP3 and AQP7 bind to the lipid droplet protein perilipin 1 (PLIN1), suggesting that PLIN1 is involved in the coordination of the subcellular translocation of aquaglyceroporins in human adipocytes. Moreover, in addition to aquaglyceroporins, we discovered by transcriptome sequencing that AQP1 is expressed in human primary adipocytes. AQP1 is mainly a water channel and thus is thought to be involved in the response to hyper-osmotic stress by efflux of water during hyperglycemia. Thus, this data suggests a contribution of both orthodox aquaporin and aquaglyceroporin in human adipocytes to maintain the homeostasis of glycerol and water during fasting and feeding.</p>}}, author = {{Huang, Peng and Hansen, Jesper S. and Saba, Karim H. and Bergman, Anna and Negoita, Florentina and Gourdon, Pontus and Hagström-Andersson, Anna and Lindkvist-Petersson, Karin}}, issn = {{0005-2736}}, keywords = {{AQP; Aquaglyceroporins; Human adipocytes; Orthodox aquaporins; Perilipin 1}}, language = {{eng}}, month = {{02}}, number = {{1}}, publisher = {{Elsevier}}, series = {{Biochimica et Biophysica Acta - Biomembranes}}, title = {{Aquaglyceroporins and orthodox aquaporins in human adipocytes}}, url = {{http://dx.doi.org/10.1016/j.bbamem.2021.183795}}, doi = {{10.1016/j.bbamem.2021.183795}}, volume = {{1864}}, year = {{2022}}, }