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Laboratory x-ray nano-computed tomography for biomedical research

Dreier, Till LU orcid ; Krüger, Robin LU ; Bernström, Gustaf ; Tran-Lundmark, Karin LU ; Gonçalves, Isabel LU orcid and Bech, Martin LU orcid (2024) In Journal of Instrumentation 19(10).
Abstract

High-resolution x-ray tomography is a common technique for biomedical research using synchrotron sources. With advancements in laboratory x-ray sources, an increasing number of experiments can be performed in the lab. In this paper, the design, implementation, and verification of a laboratory setup for x-ray nano-computed tomography is presented using a nano-focus x-ray source and high geometric magnification not requiring any optical elements. Comparing a scintillator-based detector to a photon counting detector shows a clear benefit of using photon counting detectors for these applications, where the flux of the x-ray source is limited and samples have low contrast. Sample contrast is enhanced using propagation-based phase contrast.... (More)

High-resolution x-ray tomography is a common technique for biomedical research using synchrotron sources. With advancements in laboratory x-ray sources, an increasing number of experiments can be performed in the lab. In this paper, the design, implementation, and verification of a laboratory setup for x-ray nano-computed tomography is presented using a nano-focus x-ray source and high geometric magnification not requiring any optical elements. Comparing a scintillator-based detector to a photon counting detector shows a clear benefit of using photon counting detectors for these applications, where the flux of the x-ray source is limited and samples have low contrast. Sample contrast is enhanced using propagation-based phase contrast. The resolution of the system is verified using 2D resolution charts and using Fourier Ring Correlation on reconstructed CT slices. Evaluating noise and contrast highlights the benefits of photon counting detectors and the contrast improvement through phase contrast. The implemented setup is capable of reaching sub-micron resolution and satisfying contrast in biological samples, like paraffin embedded tissue.

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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Computerized Tomography (CT) and Computed Radiography (CR), Inspection with x-rays, X-ray generators and sources
in
Journal of Instrumentation
volume
19
issue
10
article number
P10021
publisher
IOP Publishing
external identifiers
  • scopus:85207099243
ISSN
1748-0221
DOI
10.1088/1748-0221/19/10/P10021
language
English
LU publication?
yes
id
93dd7477-19ec-45c1-af78-9ed2a9bf5f18
date added to LUP
2024-12-16 14:00:30
date last changed
2024-12-16 14:01:20
@article{93dd7477-19ec-45c1-af78-9ed2a9bf5f18,
  abstract     = {{<p>High-resolution x-ray tomography is a common technique for biomedical research using synchrotron sources. With advancements in laboratory x-ray sources, an increasing number of experiments can be performed in the lab. In this paper, the design, implementation, and verification of a laboratory setup for x-ray nano-computed tomography is presented using a nano-focus x-ray source and high geometric magnification not requiring any optical elements. Comparing a scintillator-based detector to a photon counting detector shows a clear benefit of using photon counting detectors for these applications, where the flux of the x-ray source is limited and samples have low contrast. Sample contrast is enhanced using propagation-based phase contrast. The resolution of the system is verified using 2D resolution charts and using Fourier Ring Correlation on reconstructed CT slices. Evaluating noise and contrast highlights the benefits of photon counting detectors and the contrast improvement through phase contrast. The implemented setup is capable of reaching sub-micron resolution and satisfying contrast in biological samples, like paraffin embedded tissue.</p>}},
  author       = {{Dreier, Till and Krüger, Robin and Bernström, Gustaf and Tran-Lundmark, Karin and Gonçalves, Isabel and Bech, Martin}},
  issn         = {{1748-0221}},
  keywords     = {{Computerized Tomography (CT) and Computed Radiography (CR); Inspection with x-rays; X-ray generators and sources}},
  language     = {{eng}},
  number       = {{10}},
  publisher    = {{IOP Publishing}},
  series       = {{Journal of Instrumentation}},
  title        = {{Laboratory x-ray nano-computed tomography for biomedical research}},
  url          = {{http://dx.doi.org/10.1088/1748-0221/19/10/P10021}},
  doi          = {{10.1088/1748-0221/19/10/P10021}},
  volume       = {{19}},
  year         = {{2024}},
}