Advanced

Dependence of macrophage superoxide release on the pulse amplitude of an applied pressure regime : A potential factor at the soft tissue-implant interface

Shin, Hainsworth Y.; Frechette, Danielle M.; Rohner, Nathan; Zhang, Xiaoyan; Puleo, David A. and Bjursten, Lars M. LU (2016) In Journal of Tissue Engineering and Regenerative Medicine 10(3). p.227-238
Abstract

Failure of soft tissue implants has been largely attributed to the influence of biomaterial surface properties on the foreign body response, but some implant complications, e.g. macrophage accumulation and necrosis, are still not effectively addressed with surface treatments to minimize deleterious biomaterial effects. We explored an alternative explanation for implant failure, linking biocompatibility with implant micromotion-induced pressure fluctuations at the tissue-biomaterial interface. For this purpose, we used a custom in vitro system to characterize the effects of pressure fluctuations on the activity of macrophages, the predominant cells at a healing implant site. Initially, we quantified superoxide production by HL60-derived... (More)

Failure of soft tissue implants has been largely attributed to the influence of biomaterial surface properties on the foreign body response, but some implant complications, e.g. macrophage accumulation and necrosis, are still not effectively addressed with surface treatments to minimize deleterious biomaterial effects. We explored an alternative explanation for implant failure, linking biocompatibility with implant micromotion-induced pressure fluctuations at the tissue-biomaterial interface. For this purpose, we used a custom in vitro system to characterize the effects of pressure fluctuations on the activity of macrophages, the predominant cells at a healing implant site. Initially, we quantified superoxide production by HL60-derived macrophage-like cells under several different pressure regimes with means of 5-40 mmHg, amplitudes of 0-15 mmHg and frequencies of 0-1.5 Hz. All pressure regimes tested elicited significantly (p2=0.74; p

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cell adhesion, Macrophage, Mechanotransduction, Pressure, Superoxide, Tissue-implant interface
in
Journal of Tissue Engineering and Regenerative Medicine
volume
10
issue
3
pages
227 - 238
publisher
John Wiley & Sons
external identifiers
  • Scopus:84961207254
  • WOS:000372920000008
ISSN
1932-6254
DOI
10.1002/term.1789
language
English
LU publication?
yes
id
94514036-2370-44b1-a6e5-5b85712828fe
date added to LUP
2016-05-18 16:40:06
date last changed
2017-01-01 08:26:21
@article{94514036-2370-44b1-a6e5-5b85712828fe,
  abstract     = {<p>Failure of soft tissue implants has been largely attributed to the influence of biomaterial surface properties on the foreign body response, but some implant complications, e.g. macrophage accumulation and necrosis, are still not effectively addressed with surface treatments to minimize deleterious biomaterial effects. We explored an alternative explanation for implant failure, linking biocompatibility with implant micromotion-induced pressure fluctuations at the tissue-biomaterial interface. For this purpose, we used a custom in vitro system to characterize the effects of pressure fluctuations on the activity of macrophages, the predominant cells at a healing implant site. Initially, we quantified superoxide production by HL60-derived macrophage-like cells under several different pressure regimes with means of 5-40 mmHg, amplitudes of 0-15 mmHg and frequencies of 0-1.5 Hz. All pressure regimes tested elicited significantly (p2=0.74; p</p>},
  author       = {Shin, Hainsworth Y. and Frechette, Danielle M. and Rohner, Nathan and Zhang, Xiaoyan and Puleo, David A. and Bjursten, Lars M.},
  issn         = {1932-6254},
  keyword      = {Cell adhesion,Macrophage,Mechanotransduction,Pressure,Superoxide,Tissue-implant interface},
  language     = {eng},
  month        = {03},
  number       = {3},
  pages        = {227--238},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Tissue Engineering and Regenerative Medicine},
  title        = {Dependence of macrophage superoxide release on the pulse amplitude of an applied pressure regime : A potential factor at the soft tissue-implant interface},
  url          = {http://dx.doi.org/10.1002/term.1789},
  volume       = {10},
  year         = {2016},
}