Rats and mice immunised with chimeric human/mouse proteinase 3 produce autoantibodies to mouse Pr3 and rat granulocytes
van der Geld, Ymke M. ; Hellmark, Thomas LU
; Selga, Daina
LU
; Heeringa, Peter
; Huitema, Minke G.
; Limburg, Pieter C.
and Kallenberg, Cees G. M.
(2007)
In Annals of the Rheumatic Diseases
66(12).
p.1679-1682
- Abstract
- Aim: In this study, we employed chimeric human/ mouse Proteinase 3 ( PR3) proteins as tools to induce an autoantibody response to PR3 in rats and mice. Method: Rats and mice were immunised with recombinant human PR3 ( HPR3), recombinant murine PR3 ( mPR3), single chimeric human/ mouse PR3 ( HHm, HmH, mHH, mmH, mHm, Hmm) or pools of chimeric proteins. Antibodies to mPR3 and HPR3 were measured by ELISA. Antibodies to rat PR3 were determined by indirect immunofluorescence ( IIF) on rat white blood cells. Urinalysis was performed by dipstick analysis. Kidney and lung tissue was obtained for pathological examination. Results: In mice, immunisation with the chimeric human/ mouse PR3 Hmm led to an autoantibody response to mPR3. Rats immunised... (More)
- Aim: In this study, we employed chimeric human/ mouse Proteinase 3 ( PR3) proteins as tools to induce an autoantibody response to PR3 in rats and mice. Method: Rats and mice were immunised with recombinant human PR3 ( HPR3), recombinant murine PR3 ( mPR3), single chimeric human/ mouse PR3 ( HHm, HmH, mHH, mmH, mHm, Hmm) or pools of chimeric proteins. Antibodies to mPR3 and HPR3 were measured by ELISA. Antibodies to rat PR3 were determined by indirect immunofluorescence ( IIF) on rat white blood cells. Urinalysis was performed by dipstick analysis. Kidney and lung tissue was obtained for pathological examination. Results: In mice, immunisation with the chimeric human/ mouse PR3 Hmm led to an autoantibody response to mPR3. Rats immunised with the chimeric human/ mouse PR3 Hmm, HmH and mmH, or a pool of the chimeric human/ mouse PR3 proteins, produced antibodies selectively binding to rat granulocytes as detected by IIF. No gross pathological abnormalities could be detected in kidney or lungs of mice or rats immunised with chimeric human/ mouse PR3. Conclusion: Immunisation with chimeric human/ mouse proteins induces autoantibodies to PR3 in rats and mice. Chimeric proteins can be instrumental in developing experimental models for autoimmune diseases. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/972348
- author
- van der Geld, Ymke M.
; Hellmark, Thomas
LU
; Selga, Daina
LU
; Heeringa, Peter
; Huitema, Minke G.
; Limburg, Pieter C.
and Kallenberg, Cees G. M.
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Annals of the Rheumatic Diseases
- volume
- 66
- issue
- 12
- pages
- 1679 - 1682
- publisher
- BMJ Publishing Group
- external identifiers
-
- wos:000250902600024
- scopus:36749025223
- pmid:17644551
- ISSN
- 1468-2060
- DOI
- 10.1136/ard.2006.064626
- language
- English
- LU publication?
- yes
- id
- b781ec95-af3c-44cc-a5d5-d1b49321ae2b (old id 972348)
- date added to LUP
- 2016-04-01 16:45:19
- date last changed
- 2022-04-15 06:49:06
@article{b781ec95-af3c-44cc-a5d5-d1b49321ae2b,
abstract = {{Aim: In this study, we employed chimeric human/ mouse Proteinase 3 ( PR3) proteins as tools to induce an autoantibody response to PR3 in rats and mice. Method: Rats and mice were immunised with recombinant human PR3 ( HPR3), recombinant murine PR3 ( mPR3), single chimeric human/ mouse PR3 ( HHm, HmH, mHH, mmH, mHm, Hmm) or pools of chimeric proteins. Antibodies to mPR3 and HPR3 were measured by ELISA. Antibodies to rat PR3 were determined by indirect immunofluorescence ( IIF) on rat white blood cells. Urinalysis was performed by dipstick analysis. Kidney and lung tissue was obtained for pathological examination. Results: In mice, immunisation with the chimeric human/ mouse PR3 Hmm led to an autoantibody response to mPR3. Rats immunised with the chimeric human/ mouse PR3 Hmm, HmH and mmH, or a pool of the chimeric human/ mouse PR3 proteins, produced antibodies selectively binding to rat granulocytes as detected by IIF. No gross pathological abnormalities could be detected in kidney or lungs of mice or rats immunised with chimeric human/ mouse PR3. Conclusion: Immunisation with chimeric human/ mouse proteins induces autoantibodies to PR3 in rats and mice. Chimeric proteins can be instrumental in developing experimental models for autoimmune diseases.}},
author = {{van der Geld, Ymke M. and Hellmark, Thomas and Selga, Daina and Heeringa, Peter and Huitema, Minke G. and Limburg, Pieter C. and Kallenberg, Cees G. M.}},
issn = {{1468-2060}},
language = {{eng}},
number = {{12}},
pages = {{1679--1682}},
publisher = {{BMJ Publishing Group}},
series = {{Annals of the Rheumatic Diseases}},
title = {{Rats and mice immunised with chimeric human/mouse proteinase 3 produce autoantibodies to mouse Pr3 and rat granulocytes}},
url = {{http://dx.doi.org/10.1136/ard.2006.064626}},
doi = {{10.1136/ard.2006.064626}},
volume = {{66}},
year = {{2007}},
}