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Rats and mice immunised with chimeric human/mouse proteinase 3 produce autoantibodies to mouse Pr3 and rat granulocytes

van der Geld, Ymke M.; Hellmark, Thomas LU ; Selga, Daina LU ; Heeringa, Peter; Huitema, Minke G.; Limburg, Pieter C. and Kallenberg, Cees G. M. (2007) In Annals of the Rheumatic Diseases 66(12). p.1679-1682
Abstract
Aim: In this study, we employed chimeric human/ mouse Proteinase 3 ( PR3) proteins as tools to induce an autoantibody response to PR3 in rats and mice. Method: Rats and mice were immunised with recombinant human PR3 ( HPR3), recombinant murine PR3 ( mPR3), single chimeric human/ mouse PR3 ( HHm, HmH, mHH, mmH, mHm, Hmm) or pools of chimeric proteins. Antibodies to mPR3 and HPR3 were measured by ELISA. Antibodies to rat PR3 were determined by indirect immunofluorescence ( IIF) on rat white blood cells. Urinalysis was performed by dipstick analysis. Kidney and lung tissue was obtained for pathological examination. Results: In mice, immunisation with the chimeric human/ mouse PR3 Hmm led to an autoantibody response to mPR3. Rats immunised... (More)
Aim: In this study, we employed chimeric human/ mouse Proteinase 3 ( PR3) proteins as tools to induce an autoantibody response to PR3 in rats and mice. Method: Rats and mice were immunised with recombinant human PR3 ( HPR3), recombinant murine PR3 ( mPR3), single chimeric human/ mouse PR3 ( HHm, HmH, mHH, mmH, mHm, Hmm) or pools of chimeric proteins. Antibodies to mPR3 and HPR3 were measured by ELISA. Antibodies to rat PR3 were determined by indirect immunofluorescence ( IIF) on rat white blood cells. Urinalysis was performed by dipstick analysis. Kidney and lung tissue was obtained for pathological examination. Results: In mice, immunisation with the chimeric human/ mouse PR3 Hmm led to an autoantibody response to mPR3. Rats immunised with the chimeric human/ mouse PR3 Hmm, HmH and mmH, or a pool of the chimeric human/ mouse PR3 proteins, produced antibodies selectively binding to rat granulocytes as detected by IIF. No gross pathological abnormalities could be detected in kidney or lungs of mice or rats immunised with chimeric human/ mouse PR3. Conclusion: Immunisation with chimeric human/ mouse proteins induces autoantibodies to PR3 in rats and mice. Chimeric proteins can be instrumental in developing experimental models for autoimmune diseases. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Annals of the Rheumatic Diseases
volume
66
issue
12
pages
1679 - 1682
publisher
British Medical Association
external identifiers
  • wos:000250902600024
  • scopus:36749025223
ISSN
1468-2060
DOI
10.1136/ard.2006.064626
language
English
LU publication?
yes
id
b781ec95-af3c-44cc-a5d5-d1b49321ae2b (old id 972348)
date added to LUP
2008-01-30 09:42:39
date last changed
2017-09-17 07:44:11
@article{b781ec95-af3c-44cc-a5d5-d1b49321ae2b,
  abstract     = {Aim: In this study, we employed chimeric human/ mouse Proteinase 3 ( PR3) proteins as tools to induce an autoantibody response to PR3 in rats and mice. Method: Rats and mice were immunised with recombinant human PR3 ( HPR3), recombinant murine PR3 ( mPR3), single chimeric human/ mouse PR3 ( HHm, HmH, mHH, mmH, mHm, Hmm) or pools of chimeric proteins. Antibodies to mPR3 and HPR3 were measured by ELISA. Antibodies to rat PR3 were determined by indirect immunofluorescence ( IIF) on rat white blood cells. Urinalysis was performed by dipstick analysis. Kidney and lung tissue was obtained for pathological examination. Results: In mice, immunisation with the chimeric human/ mouse PR3 Hmm led to an autoantibody response to mPR3. Rats immunised with the chimeric human/ mouse PR3 Hmm, HmH and mmH, or a pool of the chimeric human/ mouse PR3 proteins, produced antibodies selectively binding to rat granulocytes as detected by IIF. No gross pathological abnormalities could be detected in kidney or lungs of mice or rats immunised with chimeric human/ mouse PR3. Conclusion: Immunisation with chimeric human/ mouse proteins induces autoantibodies to PR3 in rats and mice. Chimeric proteins can be instrumental in developing experimental models for autoimmune diseases.},
  author       = {van der Geld, Ymke M. and Hellmark, Thomas and Selga, Daina and Heeringa, Peter and Huitema, Minke G. and Limburg, Pieter C. and Kallenberg, Cees G. M.},
  issn         = {1468-2060},
  language     = {eng},
  number       = {12},
  pages        = {1679--1682},
  publisher    = {British Medical Association},
  series       = {Annals of the Rheumatic Diseases},
  title        = {Rats and mice immunised with chimeric human/mouse proteinase 3 produce autoantibodies to mouse Pr3 and rat granulocytes},
  url          = {http://dx.doi.org/10.1136/ard.2006.064626},
  volume       = {66},
  year         = {2007},
}