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Characterization of the Biological Activity of the Ethanolic Extract from the Roots of Cannabis sativa L. Grown in Aeroponics

Ferrini, Fabio ; Donati Zeppa, Sabrina ; Fraternale, Daniele ; Carrabs, Vittoria ; Annibalini, Giosuè ; Verardo, Giancarlo ; Gorassini, Andrea ; Albertini, Maria Cristina ; Ismail, Tariq LU orcid and Fimognari, Carmela , et al. (2022) In Antioxidants 11(5).
Abstract

Cannabis sativa var. Kompolti, a variety routinely used for food production purposes, is characterized by a low concentration of psychoactive molecules, although containing many other biologically attractive metabolites in all parts of the plant, including the roots. In the present work, we evaluate the specific biological activities of the roots’ extract from plants cultivated through aeroponics, an affordable and reliable method facilitating the isolation and processing of roots, with the advantage of being suitable for industrial scale‐up. Furthermore, aeroponics results in an increased net accumulation of the most biologically attractive constituents (β‐sitosterol, friedelin and epi‐friedelanol) found in the roots. The ethanolic... (More)

Cannabis sativa var. Kompolti, a variety routinely used for food production purposes, is characterized by a low concentration of psychoactive molecules, although containing many other biologically attractive metabolites in all parts of the plant, including the roots. In the present work, we evaluate the specific biological activities of the roots’ extract from plants cultivated through aeroponics, an affordable and reliable method facilitating the isolation and processing of roots, with the advantage of being suitable for industrial scale‐up. Furthermore, aeroponics results in an increased net accumulation of the most biologically attractive constituents (β‐sitosterol, friedelin and epi‐friedelanol) found in the roots. The ethanolic extract of the aeroponic roots of C. sativa (APEX) and its separate components are studied to evaluate their anti‐inflammatory (modulation of the expression level of specific markers upon LPS stimulation in U937 cells, such as IL‐6, IL‐8, TNF‐α, IkB‐α, iNOS, IRAK‐1 and miR‐146a) and antioxidant (in either acellular or cellular settings) activities. The APEX anti‐inflammatory and antioxidant capacities are also functionally benchmarked using the wound‐healing assay. On the whole, the data obtained show that APEX and its main components showed significant anti‐inflammatory and antioxidant activities, which may render the exploitation of roots as a source of natural antioxidants and anti‐inflammatory agents highly attrac-tive, with the additional technical and economic advantages of aeroponics compared to soil cultivation.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
aeroponic roots, antioxidant, anti‐inflammatory, Cannabis sativa L., epi‐friedelanol, friedelin, β‐sitosterol
in
Antioxidants
volume
11
issue
5
article number
860
publisher
MDPI AG
external identifiers
  • pmid:35624724
  • scopus:85129072033
ISSN
2076-3921
DOI
10.3390/antiox11050860
language
English
LU publication?
yes
id
97e06739-d436-4596-ba76-58007374e285
date added to LUP
2022-07-04 14:12:18
date last changed
2024-06-09 21:31:52
@article{97e06739-d436-4596-ba76-58007374e285,
  abstract     = {{<p>Cannabis sativa var. Kompolti, a variety routinely used for food production purposes, is characterized by a low concentration of psychoactive molecules, although containing many other biologically attractive metabolites in all parts of the plant, including the roots. In the present work, we evaluate the specific biological activities of the roots’ extract from plants cultivated through aeroponics, an affordable and reliable method facilitating the isolation and processing of roots, with the advantage of being suitable for industrial scale‐up. Furthermore, aeroponics results in an increased net accumulation of the most biologically attractive constituents (β‐sitosterol, friedelin and epi‐friedelanol) found in the roots. The ethanolic extract of the aeroponic roots of C. sativa (APEX) and its separate components are studied to evaluate their anti‐inflammatory (modulation of the expression level of specific markers upon LPS stimulation in U937 cells, such as IL‐6, IL‐8, TNF‐α, IkB‐α, iNOS, IRAK‐1 and miR‐146a) and antioxidant (in either acellular or cellular settings) activities. The APEX anti‐inflammatory and antioxidant capacities are also functionally benchmarked using the wound‐healing assay. On the whole, the data obtained show that APEX and its main components showed significant anti‐inflammatory and antioxidant activities, which may render the exploitation of roots as a source of natural antioxidants and anti‐inflammatory agents highly attrac-tive, with the additional technical and economic advantages of aeroponics compared to soil cultivation.</p>}},
  author       = {{Ferrini, Fabio and Donati Zeppa, Sabrina and Fraternale, Daniele and Carrabs, Vittoria and Annibalini, Giosuè and Verardo, Giancarlo and Gorassini, Andrea and Albertini, Maria Cristina and Ismail, Tariq and Fimognari, Carmela and Sestili, Piero}},
  issn         = {{2076-3921}},
  keywords     = {{aeroponic roots; antioxidant; anti‐inflammatory; Cannabis sativa L.; epi‐friedelanol; friedelin; β‐sitosterol}},
  language     = {{eng}},
  number       = {{5}},
  publisher    = {{MDPI AG}},
  series       = {{Antioxidants}},
  title        = {{Characterization of the Biological Activity of the Ethanolic Extract from the Roots of Cannabis sativa L. Grown in Aeroponics}},
  url          = {{http://dx.doi.org/10.3390/antiox11050860}},
  doi          = {{10.3390/antiox11050860}},
  volume       = {{11}},
  year         = {{2022}},
}