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Phenotypic Characterization of Circulating CD4+ T Cells in ANCA-Associated Vasculitis

Lilliebladh, Sandra LU ; Johansson, Åsa LU ; Pettersson, Åsa LU ; Ohlsson, Sophie LU and Hellmark, Thomas LU (2018) In Journal of Immunology Research 2018.
Abstract

T cell-mediated immune responses are thought to play an important role in the pathogenesis of anti-neutrophil cytoplasmic antibody- (ANCA-) associated vasculitides (AAV). CD4+ T cells can be divided into subsets depending on their expression of chemokine receptors. In this study, different CD4+ T cell populations in patients with AAV were analysed and compared to healthy blood donors as well as therapy controls. 18 patients with active AAV, 46 in remission, 21 healthy controls (HBD), and 15 therapy controls (TC) were enrolled. CD4+ T cells were divided into Th1, Th2, and Th17 cells and further subdivided into naïve, central memory, effector memory, and effector cells. Regulatory T cells were also analysed. Concentrations of cytokines... (More)

T cell-mediated immune responses are thought to play an important role in the pathogenesis of anti-neutrophil cytoplasmic antibody- (ANCA-) associated vasculitides (AAV). CD4+ T cells can be divided into subsets depending on their expression of chemokine receptors. In this study, different CD4+ T cell populations in patients with AAV were analysed and compared to healthy blood donors as well as therapy controls. 18 patients with active AAV, 46 in remission, 21 healthy controls (HBD), and 15 therapy controls (TC) were enrolled. CD4+ T cells were divided into Th1, Th2, and Th17 cells and further subdivided into naïve, central memory, effector memory, and effector cells. Regulatory T cells were also analysed. Concentrations of cytokines and chemokines produced by the respective CD4+ T cell subset in plasma from 33 of the patients were measured by ELISA and compared to HBD. Clinical data were collected on all patients. CCL20 concentrations and percentages of Th17 cells (p = 0.019) were elevated in AAV patients compared to HBD. AAV patients had lower percentages of naïve CD4+ T cells (p = 0.0016) and a corresponding increase in proportion of effector memory CD4+ T cells when comparing to HBD (p = 0.027). Therapy controls showed similar results as AAV patients. In this study, we found that CD4+ T cell phenotype distribution is altered in AAV patients, in line with previously published work. However, no differences were found between AAV patients and TC, stressing the importance of treatment impact on this kind of studies.

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author
organization
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type
Contribution to journal
publication status
published
subject
in
Journal of Immunology Research
volume
2018
pages
12 pages
publisher
Hindawi Publishing Corporation
external identifiers
  • scopus:85060712714
ISSN
2314-7156
DOI
10.1155/2018/6984563
language
English
LU publication?
yes
id
99e37aa2-ff29-4928-b45e-b2ad6b53d9b4
date added to LUP
2019-01-07 16:08:05
date last changed
2019-03-08 02:47:47
@article{99e37aa2-ff29-4928-b45e-b2ad6b53d9b4,
  abstract     = {<p>T cell-mediated immune responses are thought to play an important role in the pathogenesis of anti-neutrophil cytoplasmic antibody- (ANCA-) associated vasculitides (AAV). CD4+ T cells can be divided into subsets depending on their expression of chemokine receptors. In this study, different CD4+ T cell populations in patients with AAV were analysed and compared to healthy blood donors as well as therapy controls. 18 patients with active AAV, 46 in remission, 21 healthy controls (HBD), and 15 therapy controls (TC) were enrolled. CD4+ T cells were divided into Th1, Th2, and Th17 cells and further subdivided into naïve, central memory, effector memory, and effector cells. Regulatory T cells were also analysed. Concentrations of cytokines and chemokines produced by the respective CD4+ T cell subset in plasma from 33 of the patients were measured by ELISA and compared to HBD. Clinical data were collected on all patients. CCL20 concentrations and percentages of Th17 cells (p = 0.019) were elevated in AAV patients compared to HBD. AAV patients had lower percentages of naïve CD4+ T cells (p = 0.0016) and a corresponding increase in proportion of effector memory CD4+ T cells when comparing to HBD (p = 0.027). Therapy controls showed similar results as AAV patients. In this study, we found that CD4+ T cell phenotype distribution is altered in AAV patients, in line with previously published work. However, no differences were found between AAV patients and TC, stressing the importance of treatment impact on this kind of studies.</p>},
  articleno    = {6984563},
  author       = {Lilliebladh, Sandra and Johansson, Åsa and Pettersson, Åsa and Ohlsson, Sophie and Hellmark, Thomas},
  issn         = {2314-7156},
  language     = {eng},
  pages        = {12},
  publisher    = {Hindawi Publishing Corporation},
  series       = {Journal of Immunology Research},
  title        = {Phenotypic Characterization of Circulating CD4+ T Cells in ANCA-Associated Vasculitis},
  url          = {http://dx.doi.org/10.1155/2018/6984563},
  volume       = {2018},
  year         = {2018},
}