Reversible Self-Assembled Monolayers with Tunable Surface Dynamics for Controlling Cell Adhesion Behavior
(2022) In ACS Applied Materials and Interfaces 14(37). p.41790-41799- Abstract
Cells adhering onto surfaces sense and respond to chemical and physical surface features. The control over cell adhesion behavior influences cell migration, proliferation, and differentiation, which are important considerations in biomaterial design for cell culture, tissue engineering, and regenerative medicine. Here, we report on a supramolecular-based approach to prepare reversible self-assembled monolayers (rSAMs) with tunable lateral mobility and dynamic control over surface composition to regulate cell adhesion behavior. These layers were prepared by incubating oxoacid-terminated thiol SAMs on gold in a pH 8 HEPES buffer solution containing different mole fractions of ω-(ethylene glycol)2-4- and ω-(GRGDS)-, α-benzamidino... (More)
Cells adhering onto surfaces sense and respond to chemical and physical surface features. The control over cell adhesion behavior influences cell migration, proliferation, and differentiation, which are important considerations in biomaterial design for cell culture, tissue engineering, and regenerative medicine. Here, we report on a supramolecular-based approach to prepare reversible self-assembled monolayers (rSAMs) with tunable lateral mobility and dynamic control over surface composition to regulate cell adhesion behavior. These layers were prepared by incubating oxoacid-terminated thiol SAMs on gold in a pH 8 HEPES buffer solution containing different mole fractions of ω-(ethylene glycol)2-4- and ω-(GRGDS)-, α-benzamidino bolaamphiphiles. Cell shape and morphology were influenced by the strength of the interactions between the amidine-functionalized amphiphiles and the oxoacid of the underlying SAMs. Dynamic control over surface composition, achieved by the addition of inert filler amphiphiles to the RGD-functionalized rSAMs, reversed the cell adhesion process. In summary, rSAMs featuring mobile bioactive ligands offer unique capabilities to influence and control cell adhesion behavior, suggesting a broad use in biomaterial design, tissue engineering, and regenerative medicine.
(Less)
- author
- organization
- publishing date
- 2022-09-21
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cell modulation, dynamic multivalency, ECM mimic, reversible cell adhesion, supported lipid bilayer
- in
- ACS Applied Materials and Interfaces
- volume
- 14
- issue
- 37
- pages
- 10 pages
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- scopus:85138080666
- pmid:36074978
- ISSN
- 1944-8244
- DOI
- 10.1021/acsami.2c12029
- language
- English
- LU publication?
- yes
- id
- 9a63d42e-1a05-463e-8e76-dcbf021b1084
- date added to LUP
- 2022-12-05 09:31:32
- date last changed
- 2024-09-20 07:52:46
@article{9a63d42e-1a05-463e-8e76-dcbf021b1084, abstract = {{<p>Cells adhering onto surfaces sense and respond to chemical and physical surface features. The control over cell adhesion behavior influences cell migration, proliferation, and differentiation, which are important considerations in biomaterial design for cell culture, tissue engineering, and regenerative medicine. Here, we report on a supramolecular-based approach to prepare reversible self-assembled monolayers (rSAMs) with tunable lateral mobility and dynamic control over surface composition to regulate cell adhesion behavior. These layers were prepared by incubating oxoacid-terminated thiol SAMs on gold in a pH 8 HEPES buffer solution containing different mole fractions of ω-(ethylene glycol)2-4- and ω-(GRGDS)-, α-benzamidino bolaamphiphiles. Cell shape and morphology were influenced by the strength of the interactions between the amidine-functionalized amphiphiles and the oxoacid of the underlying SAMs. Dynamic control over surface composition, achieved by the addition of inert filler amphiphiles to the RGD-functionalized rSAMs, reversed the cell adhesion process. In summary, rSAMs featuring mobile bioactive ligands offer unique capabilities to influence and control cell adhesion behavior, suggesting a broad use in biomaterial design, tissue engineering, and regenerative medicine.</p>}}, author = {{Yeung, Sing Yee and Sergeeva, Yulia and Pan, Guoqing and Mittler, Silvia and Ederth, Thomas and Dam, Tommy and Jönsson, Peter and El-Schich, Zahra and Wingren, Anette Gjörloff and Tillo, Adam and Hsiung Mattisson, Sabrina and Holmqvist, Bo and Stollenwerk, Maria M. and Sellergren, Börje}}, issn = {{1944-8244}}, keywords = {{cell modulation; dynamic multivalency; ECM mimic; reversible cell adhesion; supported lipid bilayer}}, language = {{eng}}, month = {{09}}, number = {{37}}, pages = {{41790--41799}}, publisher = {{The American Chemical Society (ACS)}}, series = {{ACS Applied Materials and Interfaces}}, title = {{Reversible Self-Assembled Monolayers with Tunable Surface Dynamics for Controlling Cell Adhesion Behavior}}, url = {{http://dx.doi.org/10.1021/acsami.2c12029}}, doi = {{10.1021/acsami.2c12029}}, volume = {{14}}, year = {{2022}}, }