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Reversible Self-Assembled Monolayers with Tunable Surface Dynamics for Controlling Cell Adhesion Behavior

Yeung, Sing Yee ; Sergeeva, Yulia ; Pan, Guoqing ; Mittler, Silvia ; Ederth, Thomas ; Dam, Tommy LU ; Jönsson, Peter LU ; El-Schich, Zahra ; Wingren, Anette Gjörloff LU and Tillo, Adam , et al. (2022) In ACS Applied Materials and Interfaces 14(37). p.41790-41799
Abstract

Cells adhering onto surfaces sense and respond to chemical and physical surface features. The control over cell adhesion behavior influences cell migration, proliferation, and differentiation, which are important considerations in biomaterial design for cell culture, tissue engineering, and regenerative medicine. Here, we report on a supramolecular-based approach to prepare reversible self-assembled monolayers (rSAMs) with tunable lateral mobility and dynamic control over surface composition to regulate cell adhesion behavior. These layers were prepared by incubating oxoacid-terminated thiol SAMs on gold in a pH 8 HEPES buffer solution containing different mole fractions of ω-(ethylene glycol)2-4- and ω-(GRGDS)-, α-benzamidino... (More)

Cells adhering onto surfaces sense and respond to chemical and physical surface features. The control over cell adhesion behavior influences cell migration, proliferation, and differentiation, which are important considerations in biomaterial design for cell culture, tissue engineering, and regenerative medicine. Here, we report on a supramolecular-based approach to prepare reversible self-assembled monolayers (rSAMs) with tunable lateral mobility and dynamic control over surface composition to regulate cell adhesion behavior. These layers were prepared by incubating oxoacid-terminated thiol SAMs on gold in a pH 8 HEPES buffer solution containing different mole fractions of ω-(ethylene glycol)2-4- and ω-(GRGDS)-, α-benzamidino bolaamphiphiles. Cell shape and morphology were influenced by the strength of the interactions between the amidine-functionalized amphiphiles and the oxoacid of the underlying SAMs. Dynamic control over surface composition, achieved by the addition of inert filler amphiphiles to the RGD-functionalized rSAMs, reversed the cell adhesion process. In summary, rSAMs featuring mobile bioactive ligands offer unique capabilities to influence and control cell adhesion behavior, suggesting a broad use in biomaterial design, tissue engineering, and regenerative medicine.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cell modulation, dynamic multivalency, ECM mimic, reversible cell adhesion, supported lipid bilayer
in
ACS Applied Materials and Interfaces
volume
14
issue
37
pages
10 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • scopus:85138080666
  • pmid:36074978
ISSN
1944-8244
DOI
10.1021/acsami.2c12029
language
English
LU publication?
yes
id
9a63d42e-1a05-463e-8e76-dcbf021b1084
date added to LUP
2022-12-05 09:31:32
date last changed
2024-06-28 00:04:58
@article{9a63d42e-1a05-463e-8e76-dcbf021b1084,
  abstract     = {{<p>Cells adhering onto surfaces sense and respond to chemical and physical surface features. The control over cell adhesion behavior influences cell migration, proliferation, and differentiation, which are important considerations in biomaterial design for cell culture, tissue engineering, and regenerative medicine. Here, we report on a supramolecular-based approach to prepare reversible self-assembled monolayers (rSAMs) with tunable lateral mobility and dynamic control over surface composition to regulate cell adhesion behavior. These layers were prepared by incubating oxoacid-terminated thiol SAMs on gold in a pH 8 HEPES buffer solution containing different mole fractions of ω-(ethylene glycol)2-4- and ω-(GRGDS)-, α-benzamidino bolaamphiphiles. Cell shape and morphology were influenced by the strength of the interactions between the amidine-functionalized amphiphiles and the oxoacid of the underlying SAMs. Dynamic control over surface composition, achieved by the addition of inert filler amphiphiles to the RGD-functionalized rSAMs, reversed the cell adhesion process. In summary, rSAMs featuring mobile bioactive ligands offer unique capabilities to influence and control cell adhesion behavior, suggesting a broad use in biomaterial design, tissue engineering, and regenerative medicine.</p>}},
  author       = {{Yeung, Sing Yee and Sergeeva, Yulia and Pan, Guoqing and Mittler, Silvia and Ederth, Thomas and Dam, Tommy and Jönsson, Peter and El-Schich, Zahra and Wingren, Anette Gjörloff and Tillo, Adam and Hsiung Mattisson, Sabrina and Holmqvist, Bo and Stollenwerk, Maria M. and Sellergren, Börje}},
  issn         = {{1944-8244}},
  keywords     = {{cell modulation; dynamic multivalency; ECM mimic; reversible cell adhesion; supported lipid bilayer}},
  language     = {{eng}},
  month        = {{09}},
  number       = {{37}},
  pages        = {{41790--41799}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{ACS Applied Materials and Interfaces}},
  title        = {{Reversible Self-Assembled Monolayers with Tunable Surface Dynamics for Controlling Cell Adhesion Behavior}},
  url          = {{http://dx.doi.org/10.1021/acsami.2c12029}},
  doi          = {{10.1021/acsami.2c12029}},
  volume       = {{14}},
  year         = {{2022}},
}