MicroRNA-340-5p inhibits colon cancer cell migration via targeting of RhoA
(2020) In Scientific Reports 10(1). p.16934-16934- Abstract
Colon cancer is the third most common cancer and a significant cause of cancer-related deaths worldwide. Metastasis is the most insidious aspect of cancer progression. Convincing data suggest that microRNAs (miRs) play a key function in colon cancer biology. We examined the role of miR-340-5p in regulating RhoA expression as well as cell migration and invasion in colon cancer cells. Levels of miR-340-5p and RhoA mRNA varied inversely in serum-free and serum-grown HT-29 and AZ-97 colon cancer cells. It was found transfection with miR-340-5p not only decreased expression of RhoA mRNA and protein levels in HT-29 cells but also reduced colon cancer cell migration and invasion. Bioinformatics analysis predicted one putative binding sites at... (More)
Colon cancer is the third most common cancer and a significant cause of cancer-related deaths worldwide. Metastasis is the most insidious aspect of cancer progression. Convincing data suggest that microRNAs (miRs) play a key function in colon cancer biology. We examined the role of miR-340-5p in regulating RhoA expression as well as cell migration and invasion in colon cancer cells. Levels of miR-340-5p and RhoA mRNA varied inversely in serum-free and serum-grown HT-29 and AZ-97 colon cancer cells. It was found transfection with miR-340-5p not only decreased expression of RhoA mRNA and protein levels in HT-29 cells but also reduced colon cancer cell migration and invasion. Bioinformatics analysis predicted one putative binding sites at the 3'-UTR of RhoA mRNA. Targeting this binding site with a specific blocker reversed mimic miR-340-5p-induced inhibition of RhoA activation and colon cancer cell migration and invasion. These novel results suggest that miR-340-5p is an important regulator of colon cancer cell motility via targeting of RhoA and further experiments are warranted to evaluate the role of miR-340-5p in colon cancer metastasis.
(Less)
- author
- Algaber, Anwar LU ; Al-Haidari, Amr LU ; Madhi, Raed LU ; Rahman, Milladur LU ; Syk, Ingvar LU and Thorlacius, Henrik LU
- organization
- publishing date
- 2020-10-09
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scientific Reports
- volume
- 10
- issue
- 1
- pages
- 16934 - 16934
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:33037251
- scopus:85092215909
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-020-73792-9
- language
- English
- LU publication?
- yes
- id
- 9b66d75c-3fa2-4ff4-b197-e4bfdc2ea8f8
- date added to LUP
- 2020-10-20 13:30:36
- date last changed
- 2024-07-25 02:21:27
@article{9b66d75c-3fa2-4ff4-b197-e4bfdc2ea8f8, abstract = {{<p>Colon cancer is the third most common cancer and a significant cause of cancer-related deaths worldwide. Metastasis is the most insidious aspect of cancer progression. Convincing data suggest that microRNAs (miRs) play a key function in colon cancer biology. We examined the role of miR-340-5p in regulating RhoA expression as well as cell migration and invasion in colon cancer cells. Levels of miR-340-5p and RhoA mRNA varied inversely in serum-free and serum-grown HT-29 and AZ-97 colon cancer cells. It was found transfection with miR-340-5p not only decreased expression of RhoA mRNA and protein levels in HT-29 cells but also reduced colon cancer cell migration and invasion. Bioinformatics analysis predicted one putative binding sites at the 3'-UTR of RhoA mRNA. Targeting this binding site with a specific blocker reversed mimic miR-340-5p-induced inhibition of RhoA activation and colon cancer cell migration and invasion. These novel results suggest that miR-340-5p is an important regulator of colon cancer cell motility via targeting of RhoA and further experiments are warranted to evaluate the role of miR-340-5p in colon cancer metastasis.</p>}}, author = {{Algaber, Anwar and Al-Haidari, Amr and Madhi, Raed and Rahman, Milladur and Syk, Ingvar and Thorlacius, Henrik}}, issn = {{2045-2322}}, language = {{eng}}, month = {{10}}, number = {{1}}, pages = {{16934--16934}}, publisher = {{Nature Publishing Group}}, series = {{Scientific Reports}}, title = {{MicroRNA-340-5p inhibits colon cancer cell migration via targeting of RhoA}}, url = {{http://dx.doi.org/10.1038/s41598-020-73792-9}}, doi = {{10.1038/s41598-020-73792-9}}, volume = {{10}}, year = {{2020}}, }