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MicroRNA-340-5p inhibits colon cancer cell migration via targeting of RhoA

Algaber, Anwar LU ; Al-Haidari, Amr LU ; Madhi, Raed LU ; Rahman, Milladur LU orcid ; Syk, Ingvar LU and Thorlacius, Henrik LU (2020) In Scientific Reports 10(1). p.16934-16934
Abstract

Colon cancer is the third most common cancer and a significant cause of cancer-related deaths worldwide. Metastasis is the most insidious aspect of cancer progression. Convincing data suggest that microRNAs (miRs) play a key function in colon cancer biology. We examined the role of miR-340-5p in regulating RhoA expression as well as cell migration and invasion in colon cancer cells. Levels of miR-340-5p and RhoA mRNA varied inversely in serum-free and serum-grown HT-29 and AZ-97 colon cancer cells. It was found transfection with miR-340-5p not only decreased expression of RhoA mRNA and protein levels in HT-29 cells but also reduced colon cancer cell migration and invasion. Bioinformatics analysis predicted one putative binding sites at... (More)

Colon cancer is the third most common cancer and a significant cause of cancer-related deaths worldwide. Metastasis is the most insidious aspect of cancer progression. Convincing data suggest that microRNAs (miRs) play a key function in colon cancer biology. We examined the role of miR-340-5p in regulating RhoA expression as well as cell migration and invasion in colon cancer cells. Levels of miR-340-5p and RhoA mRNA varied inversely in serum-free and serum-grown HT-29 and AZ-97 colon cancer cells. It was found transfection with miR-340-5p not only decreased expression of RhoA mRNA and protein levels in HT-29 cells but also reduced colon cancer cell migration and invasion. Bioinformatics analysis predicted one putative binding sites at the 3'-UTR of RhoA mRNA. Targeting this binding site with a specific blocker reversed mimic miR-340-5p-induced inhibition of RhoA activation and colon cancer cell migration and invasion. These novel results suggest that miR-340-5p is an important regulator of colon cancer cell motility via targeting of RhoA and further experiments are warranted to evaluate the role of miR-340-5p in colon cancer metastasis.

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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
10
issue
1
pages
16934 - 16934
publisher
Nature Publishing Group
external identifiers
  • pmid:33037251
  • scopus:85092215909
ISSN
2045-2322
DOI
10.1038/s41598-020-73792-9
language
English
LU publication?
yes
id
9b66d75c-3fa2-4ff4-b197-e4bfdc2ea8f8
date added to LUP
2020-10-20 13:30:36
date last changed
2024-02-17 00:26:27
@article{9b66d75c-3fa2-4ff4-b197-e4bfdc2ea8f8,
  abstract     = {{<p>Colon cancer is the third most common cancer and a significant cause of cancer-related deaths worldwide. Metastasis is the most insidious aspect of cancer progression. Convincing data suggest that microRNAs (miRs) play a key function in colon cancer biology. We examined the role of miR-340-5p in regulating RhoA expression as well as cell migration and invasion in colon cancer cells. Levels of miR-340-5p and RhoA mRNA varied inversely in serum-free and serum-grown HT-29 and AZ-97 colon cancer cells. It was found transfection with miR-340-5p not only decreased expression of RhoA mRNA and protein levels in HT-29 cells but also reduced colon cancer cell migration and invasion. Bioinformatics analysis predicted one putative binding sites at the 3'-UTR of RhoA mRNA. Targeting this binding site with a specific blocker reversed mimic miR-340-5p-induced inhibition of RhoA activation and colon cancer cell migration and invasion. These novel results suggest that miR-340-5p is an important regulator of colon cancer cell motility via targeting of RhoA and further experiments are warranted to evaluate the role of miR-340-5p in colon cancer metastasis.</p>}},
  author       = {{Algaber, Anwar and Al-Haidari, Amr and Madhi, Raed and Rahman, Milladur and Syk, Ingvar and Thorlacius, Henrik}},
  issn         = {{2045-2322}},
  language     = {{eng}},
  month        = {{10}},
  number       = {{1}},
  pages        = {{16934--16934}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Scientific Reports}},
  title        = {{MicroRNA-340-5p inhibits colon cancer cell migration via targeting of RhoA}},
  url          = {{http://dx.doi.org/10.1038/s41598-020-73792-9}},
  doi          = {{10.1038/s41598-020-73792-9}},
  volume       = {{10}},
  year         = {{2020}},
}